Strain Fitness in Pseudomonas putida KT2440 around PP_2129

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
2,428,098	+				+1.3
2,428,204	-		PP_2128	0.14	-0.7
2,428,204	-		PP_2128	0.14	-0.7
2,428,247	+		PP_2128	0.18	-1.3
2,428,248	-		PP_2128	0.18	-1.0
2,428,248	-		PP_2128	0.18	-0.1
2,428,248	-		PP_2128	0.18	+0.1
2,428,248	-		PP_2128	0.18	-0.1
2,428,365	-		PP_2128	0.31	-1.1
2,428,365	-		PP_2128	0.31	-0.7
2,428,393	-		PP_2128	0.34	-0.5
2,428,470	-		PP_2128	0.42	+1.3
2,428,551	-		PP_2128	0.51	-2.5
2,428,664	-		PP_2128	0.63	+0.2
2,428,862	+		PP_2128	0.84	-1.8
2,428,862	+		PP_2128	0.84	-0.6
2,428,862	+		PP_2128	0.84	+0.2
2,428,862	+		PP_2128	0.84	-0.2
2,428,862	+		PP_2128	0.84	-1.7
2,428,863	-		PP_2128	0.84	-1.2
2,428,863	-		PP_2128	0.84	-0.1
2,428,863	-		PP_2128	0.84	+0.4
2,428,863	-		PP_2128	0.84	+0.4
2,429,019	-				+0.1
2,429,029	+				+1.0
2,429,030	-				-0.0
2,429,030	-				+2.1
2,429,031	+				+0.1
2,429,031	+				-1.7
2,429,031	+				+1.1
2,429,031	+				+0.6
2,429,032	-				+0.8
2,429,032	-				-0.1
2,429,032	-				+0.6
2,429,085	+				+0.4
2,429,086	-				-0.5
2,429,431	+		PP_2129	0.45	-1.1
2,429,432	-		PP_2129	0.45	-1.5
2,429,458	-		PP_2129	0.49	-0.9
2,429,473	-		PP_2129	0.50	-2.2
2,429,562	-		PP_2129	0.62	-0.1
2,429,736	-		PP_2129	0.83	-0.3
2,429,868	+				+0.8
2,429,868	+				-0.1
2,429,868	+				+0.8
2,429,869	-				-0.4
2,429,869	-				+0.2
2,429,913	+				+0.6
2,429,936	+				+0.8
2,429,937	-				+0.2
2,430,050	+				-0.4
2,430,062	+				-1.0
2,430,079	+				+0.6
2,430,079	+				-0.7
2,430,185	+		PP_2130	0.10	-0.8
2,430,230	-		PP_2130	0.13	-3.2
2,430,230	-				+0.2
2,430,230	-		PP_2130	0.13	-1.1
2,430,230	-		PP_2130	0.13	-1.4
2,430,552	-		PP_2130	0.29	+0.6
2,430,623	+		PP_2130	0.32	-0.0
2,430,789	-		PP_2130	0.41	-0.7
2,430,818	+		PP_2130	0.42	+1.2
2,430,818	+		PP_2130	0.42	-0.0
2,430,819	-		PP_2130	0.42	+0.0