Strain Fitness in Pseudomonas putida KT2440 around PP_2004

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
2,272,328	-		PP_2003	0.25	+0.2
2,272,403	+		PP_2003	0.33	-0.3
2,272,403	+		PP_2003	0.33	+2.6
2,272,404	-		PP_2003	0.33	-0.6
2,272,694	+		PP_2003	0.64	+0.9
2,272,703	+		PP_2003	0.65	-0.2
2,272,704	-		PP_2003	0.65	-0.6
2,272,724	+		PP_2003	0.67	-1.1
2,272,724	+		PP_2003	0.67	-0.2
2,272,725	-		PP_2003	0.67	-0.2
2,272,725	-		PP_2003	0.67	-1.7
2,273,093	+				-2.1
2,273,094	-				+0.4
2,273,107	+				-1.2
2,273,107	+				-0.6
2,273,108	-				+0.6
2,273,108	-				+1.6
2,273,142	+				-2.1
2,273,149	-				+0.7
2,273,157	+				-1.2
2,273,157	+				+0.1
2,273,157	+				+2.6
2,273,157	+				-0.5
2,273,157	+				-1.3
2,273,157	+				-0.7
2,273,157	+				-1.4
2,273,158	-				+0.1
2,273,158	-				-0.8
2,273,158	-				+1.5
2,273,158	-				-2.1
2,273,190	+				+0.2
2,273,190	+				-0.6
2,273,208	+				+0.4
2,273,208	+				+1.1
2,273,208	+				-0.0
2,273,209	-				+2.0
2,273,209	-				-0.3
2,273,209	-				-0.2
2,273,209	-				-1.4
2,273,209	-				+0.0
2,273,253	-				-0.3
2,273,265	+				-0.9
2,273,265	+				+0.4
2,273,282	+				+0.5
2,273,328	-				+0.6
2,273,429	+				-0.9
2,273,429	+				-0.0
2,273,429	+				+0.3
2,273,429	+				+1.0
2,273,430	-		PP_2004	0.10	+0.4
2,273,430	-		PP_2004	0.10	-1.4
2,273,430	-		PP_2004	0.10	+2.6
2,273,430	-		PP_2004	0.10	-0.4
2,273,430	-		PP_2004	0.10	+0.8
2,273,434	+		PP_2004	0.10	+0.3
2,273,435	-		PP_2004	0.10	-1.4
2,273,435	-		PP_2004	0.10	-0.2
2,273,468	+		PP_2004	0.14	-1.4
2,273,468	+		PP_2004	0.14	+0.4
2,273,468	+		PP_2004	0.14	-1.4
2,273,469	-		PP_2004	0.14	-1.0
2,273,469	-		PP_2004	0.14	-0.1
2,273,469	-		PP_2004	0.14	+0.1
2,273,560	+		PP_2004	0.22	-1.9
2,273,560	+		PP_2004	0.22	-2.0
2,273,561	-		PP_2004	0.23	-0.2
2,273,816	+		PP_2004	0.47	-0.8
2,273,828	+		PP_2004	0.48	-1.3
2,273,828	+		PP_2004	0.48	-1.8
2,273,828	+		PP_2004	0.48	+0.1
2,273,829	-				+2.6
2,273,942	+		PP_2004	0.59	+0.4
2,273,951	+		PP_2004	0.60	-0.4
2,273,951	+		PP_2004	0.60	-2.2
2,273,951	+		PP_2004	0.60	-1.2
2,273,966	+		PP_2004	0.61	+0.2
2,273,966	+		PP_2004	0.61	-0.4
2,273,966	+		PP_2004	0.61	-2.3
2,274,141	+		PP_2004	0.78	-0.1
2,274,142	-		PP_2004	0.78	-1.0
2,274,142	-		PP_2004	0.78	-1.8
2,274,152	-		PP_2004	0.79	-0.6
2,274,200	+		PP_2004	0.84	-2.3
2,274,201	-		PP_2004	0.84	+0.1
2,274,201	-		PP_2004	0.84	+0.4
2,274,201	-		PP_2004	0.84	+0.8
2,274,373	-				+0.4
2,274,671	+		PP_2005	0.27	-0.1
2,274,909	-		PP_2005	0.48	+0.7
2,274,909	-		PP_2005	0.48	-0.7
2,274,924	+		PP_2005	0.49	-0.4
2,274,924	+		PP_2005	0.49	-0.0
2,275,002	+		PP_2005	0.56	-0.2
2,275,002	+		PP_2005	0.56	-1.0
2,275,003	-		PP_2005	0.56	+0.8
2,275,149	+		PP_2005	0.70	-0.0
2,275,149	+		PP_2005	0.70	-0.4
2,275,149	+		PP_2005	0.70	-1.0
2,275,150	-		PP_2005	0.70	+0.8
2,275,187	-		PP_2005	0.73	+0.2
2,275,308	+		PP_2005	0.84	+0.1