Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1732 and PP_1733 are separated by 3 nucleotides PP_1733 and PP_1734 are separated by 109 nucleotides PP_1734 and PP_1735 are separated by 45 nucleotides
PP_1732: PP_1732 - Cell division topological specificity factor, at 1,932,683 to 1,932,937
_1732
PP_1733: PP_1733 - membrane ATPase of the MinC-MinD-MinE system, at 1,932,941 to 1,933,753
_1733
PP_1734: PP_1734 - Probable septum site-determining protein MinC, at 1,933,863 to 1,934,690
_1734
PP_1735: PP_1735 - Lipid A biosynthesis lauroyl acyltransferase, at 1,934,736 to 1,935,671
_1735
Position (kb) 1933
1934
1935 Strain fitness (log2 ratio) -1
0
1
2
3 at 1933.042 kb on - strand, within PP_1733 at 1933.042 kb on - strand, within PP_1733 at 1933.307 kb on + strand, within PP_1733 at 1933.357 kb on - strand, within PP_1733 at 1933.410 kb on + strand, within PP_1733 at 1933.411 kb on - strand, within PP_1733 at 1933.503 kb on + strand, within PP_1733 at 1933.503 kb on + strand, within PP_1733 at 1933.503 kb on + strand, within PP_1733 at 1933.504 kb on - strand, within PP_1733 at 1933.504 kb on - strand at 1933.581 kb on + strand, within PP_1733 at 1933.699 kb on + strand at 1933.700 kb on - strand at 1933.700 kb on - strand at 1933.733 kb on - strand at 1933.733 kb on - strand at 1933.774 kb on - strand at 1933.812 kb on - strand at 1933.812 kb on - strand at 1933.815 kb on + strand at 1933.815 kb on + strand at 1933.816 kb on - strand at 1933.828 kb on - strand at 1933.858 kb on - strand at 1934.006 kb on + strand at 1934.007 kb on - strand, within PP_1734 at 1934.063 kb on - strand, within PP_1734 at 1934.143 kb on + strand, within PP_1734 at 1934.143 kb on + strand, within PP_1734 at 1934.143 kb on + strand, within PP_1734 at 1934.144 kb on - strand, within PP_1734 at 1934.144 kb on - strand, within PP_1734 at 1934.144 kb on - strand at 1934.144 kb on - strand, within PP_1734 at 1934.174 kb on + strand, within PP_1734 at 1934.174 kb on + strand, within PP_1734 at 1934.460 kb on - strand, within PP_1734 at 1934.460 kb on - strand, within PP_1734 at 1934.460 kb on - strand, within PP_1734 at 1934.549 kb on + strand, within PP_1734 at 1934.549 kb on + strand, within PP_1734 at 1934.550 kb on - strand, within PP_1734 at 1934.624 kb on + strand at 1934.625 kb on - strand at 1934.625 kb on - strand at 1934.657 kb on + strand at 1934.657 kb on + strand at 1934.658 kb on - strand at 1934.658 kb on - strand at 1934.660 kb on - strand at 1934.660 kb on - strand at 1934.760 kb on + strand at 1935.652 kb on - strand at 1935.681 kb on + strand at 1935.682 kb on - strand at 1935.682 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. remove 1,933,042 - PP_1733 0.12 +2.6 1,933,042 - PP_1733 0.12 +2.6 1,933,307 + PP_1733 0.45 +2.6 1,933,357 - PP_1733 0.51 +2.6 1,933,410 + PP_1733 0.58 -0.4 1,933,411 - PP_1733 0.58 +1.6 1,933,503 + PP_1733 0.69 +2.6 1,933,503 + PP_1733 0.69 +2.6 1,933,503 + PP_1733 0.69 +2.6 1,933,504 - PP_1733 0.69 +1.6 1,933,504 - +2.6 1,933,581 + PP_1733 0.79 +1.0 1,933,699 + +2.6 1,933,700 - +0.2 1,933,700 - +1.6 1,933,733 - +2.6 1,933,733 - +1.0 1,933,774 - +0.6 1,933,812 - +2.0 1,933,812 - -1.0 1,933,815 + +2.6 1,933,815 + +2.6 1,933,816 - -0.6 1,933,828 - +0.0 1,933,858 - -0.1 1,934,006 + +1.0 1,934,007 - PP_1734 0.17 +2.6 1,934,063 - PP_1734 0.24 -0.6 1,934,143 + PP_1734 0.34 +2.6 1,934,143 + PP_1734 0.34 +2.6 1,934,143 + PP_1734 0.34 +2.6 1,934,144 - PP_1734 0.34 +2.6 1,934,144 - PP_1734 0.34 +2.6 1,934,144 - +2.6 1,934,144 - PP_1734 0.34 +2.6 1,934,174 + PP_1734 0.38 +1.0 1,934,174 + PP_1734 0.38 +2.6 1,934,460 - PP_1734 0.72 +2.6 1,934,460 - PP_1734 0.72 +2.6 1,934,460 - PP_1734 0.72 +1.6 1,934,549 + PP_1734 0.83 +2.6 1,934,549 + PP_1734 0.83 +2.6 1,934,550 - PP_1734 0.83 +2.6 1,934,624 + -0.4 1,934,625 - +0.6 1,934,625 - +2.6 1,934,657 + +2.6 1,934,657 + +2.6 1,934,658 - +1.2 1,934,658 - +0.6 1,934,660 - +3.6 1,934,660 - +0.2 1,934,760 + +0.2 1,935,652 - +1.0 1,935,681 + -0.7 1,935,682 - +2.6 1,935,682 - -0.5
Or see this region's nucleotide sequence