Strain Fitness in Pseudomonas putida KT2440 around PP_1353

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
1,541,283	+		PP_1351	0.74	+2.6
1,541,283	-		PP_1351	0.74	-0.1
1,541,284	-		PP_1351	0.74	-0.4
1,541,284	-		PP_1351	0.74	-0.5
1,541,403	+		PP_1351	0.87	-0.6
1,541,403	+		PP_1351	0.87	+2.6
1,541,404	-		PP_1351	0.87	-1.0
1,541,404	-		PP_1351	0.87	+1.3
1,541,404	-		PP_1351	0.87	+0.1
1,541,506	+				+1.2
1,541,524	+				-0.4
1,541,524	+				+0.2
1,541,524	+				+2.6
1,541,525	-				-0.0
1,541,543	+				+1.2
1,541,544	-				+0.5
1,541,544	-				-3.0
1,541,544	-				+0.0
1,541,545	+				+1.0
1,541,545	+				-0.4
1,541,545	+				-0.0
1,541,545	+				+1.0
1,541,545	+				+0.6
1,541,546	-				+0.6
1,541,546	-				-0.4
1,541,546	-				-2.0
1,541,713	-		PP_1352	0.18	-0.5
1,541,874	+		PP_1352	0.51	-0.9
1,541,980	+		PP_1352	0.73	-0.0
1,541,981	-		PP_1352	0.73	-0.0
1,541,981	-		PP_1352	0.73	-0.9
1,542,008	-		PP_1352	0.79	+0.8
1,542,169	+				+0.1
1,542,170	-				+2.6
1,542,172	+				+0.7
1,542,172	+				+0.1
1,542,198	+				-0.4
1,542,199	-				+2.6
1,542,297	-				+2.6
1,542,658	+				+2.6
1,542,742	+		PP_1353	0.61	+2.6
1,542,742	+		PP_1353	0.61	+2.6
1,542,742	+		PP_1353	0.61	+1.6
1,542,797	+		PP_1353	0.68	+1.6
1,542,798	-		PP_1353	0.68	+2.6
1,542,951	-		PP_1353	0.86	+2.6
1,542,980	+		PP_1353	0.89	+2.6
1,542,980	+		PP_1353	0.89	+2.6
1,542,980	+		PP_1353	0.89	+2.6
1,542,981	-		PP_1353	0.90	+2.6
1,543,067	+				+1.4
1,543,067	+				-0.9
1,543,067	+				-0.9
1,543,068	-				-0.5
1,543,122	+				-0.5
1,543,123	-				-0.2
1,543,123	-				-0.5
1,543,123	-				-1.5
1,543,123	-				+0.6
1,543,159	+				-1.0
1,543,160	-				-1.6
1,543,160	-				-1.3
1,543,160	-				+0.6
1,543,161	+				+0.2
1,543,161	+				-1.5
1,543,161	+				-1.1
1,543,161	+				-1.8
1,543,162	-				+0.3
1,543,162	-				-1.7
1,543,162	-				+0.1
1,543,212	+				+0.5
1,543,212	+				-1.4
1,543,212	+				-1.0
1,543,212	+				+0.2
1,543,212	+				+1.0
1,543,212	+				-1.3
1,543,213	-				-1.0
1,543,213	-				+0.4
1,543,213	-				-1.0
1,543,213	-				+0.0
1,543,213	-				-0.1
1,543,213	-				-0.2
1,543,214	+				-0.1
1,543,215	-				-1.7
1,543,215	-				+1.2
1,543,275	+				-0.7
1,543,275	+				+0.1
1,543,276	-				+0.6
1,543,276	-				-0.4
1,543,344	+		PP_1354	0.12	+0.0
1,543,344	+		PP_1354	0.12	-0.0
1,543,344	+		PP_1354	0.12	+0.4
1,543,344	+		PP_1354	0.12	-1.4
1,543,344	+		PP_1354	0.12	-1.4
1,543,344	+		PP_1354	0.12	-1.2
1,543,345	-		PP_1354	0.12	-0.5
1,543,348	+		PP_1354	0.12	+0.4
1,543,348	+		PP_1354	0.12	+1.2
1,543,349	-		PP_1354	0.12	-0.3
1,543,369	-		PP_1354	0.14	+0.3
1,543,387	+		PP_1354	0.15	-1.3
1,543,388	-		PP_1354	0.15	+0.1
1,543,607	-		PP_1354	0.33	-0.4
1,543,607	-		PP_1354	0.33	+0.9
1,543,752	+		PP_1354	0.45	-0.6
1,543,980	+		PP_1354	0.63	-1.1
1,543,981	-		PP_1354	0.63	+0.2
1,543,981	-		PP_1354	0.63	-1.0
1,543,981	-		PP_1354	0.63	+1.0
1,544,043	-		PP_1354	0.68	+0.1