Strain Fitness in Pseudomonas putida KT2440 around PP_1145

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
1,310,991	+		PP_1144	0.76	-0.6
1,311,004	-		PP_1144	0.77	+0.1
1,311,004	-		PP_1144	0.77	-1.0
1,311,050	+		PP_1144	0.79	-0.1
1,311,050	+		PP_1144	0.79	-0.8
1,311,050	+		PP_1144	0.79	-0.7
1,311,051	-		PP_1144	0.79	-0.5
1,311,069	+		PP_1144	0.80	-1.1
1,311,069	+		PP_1144	0.80	-1.7
1,311,070	-		PP_1144	0.80	+1.0
1,311,160	-		PP_1144	0.84	-1.3
1,311,160	-		PP_1144	0.84	+1.6
1,311,248	+		PP_1144	0.87	-0.6
1,311,249	-		PP_1144	0.87	+0.5
1,311,249	-		PP_1144	0.87	-0.2
1,311,255	+		PP_1144	0.88	-0.9
1,311,255	+		PP_1144	0.88	-0.7
1,311,255	+				+1.0
1,311,255	+		PP_1144	0.88	+0.6
1,311,256	-		PP_1144	0.88	+0.4
1,311,256	-		PP_1144	0.88	+0.1
1,311,256	-		PP_1144	0.88	-0.7
1,311,311	+				-0.9
1,311,311	+				+1.6
1,311,311	+				-0.5
1,311,312	-				-0.2
1,311,312	-				-0.0
1,311,570	-				-1.0
1,311,599	+				-0.4
1,311,599	+				-0.2
1,311,599	+				+0.1
1,311,599	+				-0.7
1,311,600	-				-1.0
1,311,600	-				-1.2
1,311,601	+				+1.3
1,311,601	+				-0.0
1,311,601	+				+0.3
1,311,602	-				+0.3
1,311,602	-				-0.7
1,311,602	-				-0.7
1,311,602	-				-0.2
1,311,611	+				-1.3
1,311,612	-				+0.1
1,311,612	-				+0.6
1,311,615	+				-1.3
1,311,616	-				-0.5
1,311,630	-				+0.6
1,311,630	-				-0.1
1,311,630	-				+1.0
1,311,660	+				+0.4
1,311,683	-				+1.1
1,311,696	+				-2.0
1,311,696	+				+0.3
1,311,697	-				+0.1
1,312,049	+				-1.0
1,312,049	+				-1.9
1,312,050	-				-0.2
1,312,208	-		PP_1145	0.14	-0.4
1,312,208	-		PP_1145	0.14	+3.6
1,312,359	+		PP_1145	0.19	+1.0
1,312,360	-		PP_1145	0.19	+0.2
1,312,423	+		PP_1145	0.22	+0.3
1,312,424	-		PP_1145	0.22	-0.2
1,312,424	-		PP_1145	0.22	+0.5
1,312,424	-		PP_1145	0.22	-0.3
1,312,559	-		PP_1145	0.26	+0.6
1,312,559	-		PP_1145	0.26	+0.6
1,312,559	-		PP_1145	0.26	+1.9
1,312,834	+		PP_1145	0.36	+0.8
1,312,834	+		PP_1145	0.36	+0.2
1,312,835	-		PP_1145	0.36	+0.7
1,312,874	-		PP_1145	0.37	-0.0
1,312,892	+		PP_1145	0.38	-1.3
1,313,308	+		PP_1145	0.53	-0.1
1,313,737	-		PP_1145	0.68	+0.1
1,313,737	-		PP_1145	0.68	+0.7
1,313,737	-		PP_1145	0.68	+0.3
1,313,737	-		PP_1145	0.68	-2.0
1,314,048	+		PP_1145	0.79	+1.8
1,314,268	+				+2.6
1,314,269	-		PP_1145	0.86	+0.7
1,314,297	+		PP_1145	0.87	+0.6
1,314,297	+		PP_1145	0.87	+1.1
1,314,298	-		PP_1145	0.87	-1.9
1,314,298	-		PP_1145	0.87	-0.6
1,314,298	-		PP_1145	0.87	+1.6
1,314,403	+				+2.2
1,314,404	-				+0.6
1,314,523	+				-0.2
1,314,551	-				+1.1
1,314,551	-				+0.9
1,314,643	+				-0.2
1,314,689	+				+1.3
1,314,690	-				+0.9
1,314,690	-				-2.2
1,314,726	+				-0.3
1,314,756	+				+0.0
1,314,757	-				+1.0
1,314,928	+		PP_1146	0.17	+1.0
1,314,929	-		PP_1146	0.18	+0.8
1,315,075	-		PP_1146	0.39	-1.7
1,315,167	+		PP_1146	0.52	-1.9
1,315,167	+		PP_1146	0.52	+1.1
1,315,167	+		PP_1146	0.52	-0.5
1,315,167	+		PP_1146	0.52	+0.5
1,315,167	+		PP_1146	0.52	+1.4
1,315,167	+		PP_1146	0.52	+1.8
1,315,167	+		PP_1146	0.52	+1.6
1,315,168	-		PP_1146	0.52	+1.3
1,315,168	-		PP_1146	0.52	-1.3
1,315,168	-		PP_1146	0.52	-0.9
1,315,168	-		PP_1146	0.52	-1.0
1,315,168	-		PP_1146	0.52	-0.4
1,315,170	-		PP_1146	0.52	+0.4
1,315,302	+		PP_1146	0.71	+0.2
1,315,303	-		PP_1146	0.71	-0.7
1,315,303	-		PP_1146	0.71	-0.7
1,315,311	-		PP_1146	0.72	+1.6
1,315,399	+		PP_1146	0.85	-1.7
1,315,400	-		PP_1146	0.85	-2.2
1,315,412	+		PP_1146	0.87	-0.3
1,315,412	+		PP_1146	0.87	-0.5
1,315,413	-		PP_1146	0.87	+0.7
1,315,437	+				+0.1
1,315,437	+				-0.2
1,315,437	+				-0.6
1,315,438	-				-1.4
1,315,438	-				+0.6
1,315,438	-				+1.0
1,315,438	-				-0.7
1,315,524	+				-0.0
1,315,524	+				+0.2
1,315,553	+				-0.1
1,315,553	+				+0.8
1,315,553	+				+1.1
1,315,553	+				+0.3
1,315,553	+				+0.9