Strain Fitness in Pseudomonas putida KT2440 around PP_1009

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
1,149,192	-				+0.9
1,149,344	+		PP_1008	0.20	-0.7
1,149,344	+		PP_1008	0.20	+0.5
1,149,345	-		PP_1008	0.20	+0.6
1,149,836	-				+0.2
1,150,077	+				+2.7
1,150,077	+				+1.0
1,150,435	-		PP_1009	0.42	+0.1
1,150,464	+		PP_1009	0.45	-0.3
1,150,464	+		PP_1009	0.45	-0.0
1,150,465	-		PP_1009	0.45	+0.8
1,150,465	-		PP_1009	0.45	-2.1
1,150,609	-				+1.7
1,150,610	+		PP_1009	0.60	-0.2
1,150,610	+		PP_1009	0.60	+1.1
1,150,611	-		PP_1009	0.60	+0.1
1,150,611	-		PP_1009	0.60	-0.3
1,150,611	-		PP_1009	0.60	+0.1
1,150,863	+		PP_1009	0.85	+0.1
1,150,864	-		PP_1009	0.85	-1.3
1,150,864	-		PP_1009	0.85	+0.3
1,150,864	-		PP_1009	0.85	-1.4
1,150,945	+				-1.3
1,150,946	-				-0.8
1,150,947	+				+2.4
1,150,947	+				+0.2
1,150,948	-				+1.7
1,151,017	+				-0.9
1,151,018	-				-1.2
1,151,018	-				+1.7
1,151,018	-				+2.7
1,151,196	+				-0.2
1,151,196	+				-1.0
1,151,252	+				-0.5
1,151,258	+				-1.1