Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0854 and PP_0855 are separated by 39 nucleotides PP_0855 and PP_0856 are separated by 0 nucleotides
PP_0854: PP_0854 - Histidine--tRNA ligase, at 988,149 to 989,438
_0854
PP_0855: PP_0855 - conserved protein of unknown function, at 989,478 to 990,119
_0855
PP_0856: PP_0856 - conserved lipoprotein of unknown function, at 990,120 to 991,262
_0856
Position (kb) 989
990
991 Strain fitness (log2 ratio) -1
0
1
2
3 at 989.436 kb on + strand at 989.471 kb on + strand at 989.471 kb on + strand at 989.472 kb on - strand at 989.604 kb on + strand, within PP_0855 at 989.605 kb on - strand, within PP_0855 at 989.745 kb on + strand, within PP_0855 at 989.746 kb on - strand, within PP_0855 at 989.755 kb on - strand, within PP_0855 at 989.890 kb on + strand, within PP_0855 at 990.104 kb on + strand at 990.117 kb on + strand at 990.117 kb on + strand at 990.118 kb on - strand at 990.118 kb on - strand at 990.160 kb on + strand at 990.288 kb on + strand, within PP_0856 at 990.288 kb on + strand, within PP_0856 at 990.288 kb on + strand, within PP_0856 at 990.289 kb on - strand, within PP_0856 at 990.289 kb on - strand, within PP_0856 at 990.323 kb on + strand, within PP_0856 at 990.323 kb on + strand, within PP_0856 at 990.327 kb on + strand, within PP_0856 at 990.327 kb on + strand, within PP_0856 at 990.328 kb on - strand, within PP_0856 at 990.431 kb on - strand, within PP_0856 at 990.435 kb on + strand, within PP_0856 at 990.728 kb on - strand, within PP_0856 at 990.855 kb on + strand, within PP_0856 at 990.855 kb on + strand, within PP_0856 at 990.936 kb on + strand, within PP_0856 at 990.969 kb on + strand, within PP_0856 at 991.086 kb on + strand, within PP_0856
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. remove 989,436 + -0.9 989,471 + -0.4 989,471 + +0.7 989,472 - +2.7 989,604 + PP_0855 0.20 +2.7 989,605 - PP_0855 0.20 +2.7 989,745 + PP_0855 0.42 +3.2 989,746 - PP_0855 0.42 +2.7 989,755 - PP_0855 0.43 +2.7 989,890 + PP_0855 0.64 +1.4 990,104 + +2.7 990,117 + +0.9 990,117 + +2.7 990,118 - +2.7 990,118 - +0.3 990,160 + +2.1 990,288 + PP_0856 0.15 +2.7 990,288 + PP_0856 0.15 +2.1 990,288 + PP_0856 0.15 +3.7 990,289 - PP_0856 0.15 +2.7 990,289 - PP_0856 0.15 +2.7 990,323 + PP_0856 0.18 +2.7 990,323 + PP_0856 0.18 +2.7 990,327 + PP_0856 0.18 +1.7 990,327 + PP_0856 0.18 +3.7 990,328 - PP_0856 0.18 +2.7 990,431 - PP_0856 0.27 +2.7 990,435 + PP_0856 0.28 +1.7 990,728 - PP_0856 0.53 +2.7 990,855 + PP_0856 0.64 +1.1 990,855 + PP_0856 0.64 +2.7 990,936 + PP_0856 0.71 +2.7 990,969 + PP_0856 0.74 +1.7 991,086 + PP_0856 0.85 -0.7
Or see this region's nucleotide sequence