Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0800 and PP_0801 overlap by 22 nucleotides PP_0801 and PP_0802 are separated by 88 nucleotides PP_0802 and PP_0803 are separated by 92 nucleotides
PP_0800: PP_0800 - conserved protein of unknown function, at 919,720 to 920,472
_0800
PP_0801: PP_0801 - conserved exported protein of unknown function, at 920,451 to 920,762
_0801
PP_0802: PP_0802 - putative chemotaxis protein, at 920,851 to 921,750
_0802
PP_0803: PP_0803 - Protein secretion ABC efflux system, membrane fusion protein, at 921,843 to 923,027
_0803
Position (kb) 920
921
922 Strain fitness (log2 ratio) -2
-1
0
1
2 at 919.889 kb on + strand, within PP_0800 at 919.890 kb on - strand, within PP_0800 at 919.890 kb on - strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.157 kb on - strand, within PP_0800 at 920.157 kb on - strand, within PP_0800 at 920.157 kb on - strand, within PP_0800 at 920.157 kb on - strand, within PP_0800 at 920.335 kb on + strand, within PP_0800 at 920.335 kb on + strand, within PP_0800 at 920.407 kb on - strand at 920.428 kb on - strand at 920.440 kb on + strand at 920.440 kb on + strand at 920.440 kb on + strand at 920.440 kb on + strand at 920.441 kb on - strand at 920.441 kb on - strand at 920.507 kb on - strand, within PP_0801 at 920.801 kb on - strand at 920.847 kb on + strand at 920.847 kb on + strand at 920.847 kb on + strand at 920.848 kb on - strand at 920.848 kb on - strand at 920.848 kb on - strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.850 kb on - strand at 920.925 kb on + strand at 921.002 kb on + strand, within PP_0802 at 921.002 kb on + strand, within PP_0802 at 921.002 kb on + strand, within PP_0802 at 921.002 kb on + strand, within PP_0802 at 921.003 kb on - strand, within PP_0802 at 921.237 kb on + strand, within PP_0802 at 921.237 kb on + strand, within PP_0802 at 921.319 kb on + strand, within PP_0802 at 921.460 kb on + strand, within PP_0802 at 921.461 kb on - strand, within PP_0802 at 921.461 kb on - strand, within PP_0802 at 921.601 kb on + strand, within PP_0802 at 921.601 kb on + strand, within PP_0802 at 921.602 kb on - strand, within PP_0802 at 921.602 kb on - strand, within PP_0802 at 922.021 kb on + strand, within PP_0803 at 922.021 kb on + strand, within PP_0803 at 922.068 kb on + strand, within PP_0803 at 922.093 kb on + strand, within PP_0803 at 922.094 kb on - strand, within PP_0803 at 922.094 kb on - strand, within PP_0803 at 922.096 kb on - strand, within PP_0803 at 922.096 kb on - strand, within PP_0803 at 922.103 kb on - strand, within PP_0803 at 922.103 kb on - strand, within PP_0803 at 922.108 kb on + strand, within PP_0803 at 922.108 kb on + strand, within PP_0803 at 922.109 kb on - strand, within PP_0803 at 922.400 kb on - strand, within PP_0803 at 922.588 kb on + strand, within PP_0803 at 922.588 kb on + strand, within PP_0803 at 922.589 kb on - strand, within PP_0803 at 922.589 kb on - strand, within PP_0803 at 922.706 kb on - strand, within PP_0803
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. remove 919,889 + PP_0800 0.22 -0.3 919,890 - PP_0800 0.23 +1.3 919,890 - PP_0800 0.23 -0.5 920,156 + PP_0800 0.58 +0.4 920,156 + PP_0800 0.58 -2.1 920,156 + PP_0800 0.58 -0.1 920,156 + PP_0800 0.58 -1.1 920,156 + PP_0800 0.58 +0.4 920,156 + PP_0800 0.58 -1.0 920,157 - PP_0800 0.58 +1.3 920,157 - PP_0800 0.58 -0.9 920,157 - PP_0800 0.58 -2.5 920,157 - PP_0800 0.58 -1.0 920,335 + PP_0800 0.82 +0.3 920,335 + PP_0800 0.82 -1.2 920,407 - +0.5 920,428 - -0.2 920,440 + +0.1 920,440 + +1.0 920,440 + -0.5 920,440 + -0.3 920,441 - +2.7 920,441 - -1.4 920,507 - PP_0801 0.18 -0.3 920,801 - -1.1 920,847 + +0.1 920,847 + -0.4 920,847 + -0.4 920,848 - +0.3 920,848 - +0.3 920,848 - +0.4 920,849 + -0.9 920,849 + -0.9 920,849 + -0.9 920,849 + -0.3 920,849 + -0.1 920,849 + -0.0 920,850 - -0.1 920,925 + +0.7 921,002 + PP_0802 0.17 +1.7 921,002 + PP_0802 0.17 +1.1 921,002 + PP_0802 0.17 -0.3 921,002 + PP_0802 0.17 -0.9 921,003 - PP_0802 0.17 -0.2 921,237 + PP_0802 0.43 -1.2 921,237 + PP_0802 0.43 +1.7 921,319 + PP_0802 0.52 +0.1 921,460 + PP_0802 0.68 +1.7 921,461 - PP_0802 0.68 +2.1 921,461 - PP_0802 0.68 +1.1 921,601 + PP_0802 0.83 -0.2 921,601 + PP_0802 0.83 +1.3 921,602 - PP_0802 0.83 -1.0 921,602 - PP_0802 0.83 +0.3 922,021 + PP_0803 0.15 +2.4 922,021 + PP_0803 0.15 +0.9 922,068 + PP_0803 0.19 +0.9 922,093 + PP_0803 0.21 +0.5 922,094 - PP_0803 0.21 +1.5 922,094 - PP_0803 0.21 +1.6 922,096 - PP_0803 0.21 +0.7 922,096 - PP_0803 0.21 +2.0 922,103 - PP_0803 0.22 +1.4 922,103 - PP_0803 0.22 +1.7 922,108 + PP_0803 0.22 -0.0 922,108 + PP_0803 0.22 +0.6 922,109 - PP_0803 0.22 +0.9 922,400 - PP_0803 0.47 +1.4 922,588 + PP_0803 0.63 +1.7 922,588 + PP_0803 0.63 +0.9 922,589 - PP_0803 0.63 +1.5 922,589 - PP_0803 0.63 +2.0 922,706 - PP_0803 0.73 +2.5
Or see this region's nucleotide sequence