Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0761 and PP_0762 are separated by 54 nucleotides PP_0762 and PP_0763 are separated by 293 nucleotides
PP_0761: PP_0761 - Ribosomal RNA small subunit methyltransferase C, at 876,160 to 877,158
_0761
PP_0762: PP_0762 - Glycerate dehydrogenase, at 877,213 to 878,178
_0762
PP_0763: PP_0763 - Medium-chain-fatty-acid CoA ligase, at 878,472 to 880,154
_0763
Position (kb) 877
878
879 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 876.338 kb on + strand, within PP_0761 at 876.346 kb on - strand, within PP_0761 at 876.379 kb on + strand, within PP_0761 at 876.379 kb on + strand, within PP_0761 at 876.379 kb on + strand, within PP_0761 at 876.379 kb on + strand, within PP_0761 at 876.380 kb on - strand, within PP_0761 at 876.381 kb on + strand, within PP_0761 at 876.382 kb on - strand, within PP_0761 at 876.382 kb on - strand, within PP_0761 at 876.382 kb on - strand, within PP_0761 at 876.621 kb on + strand, within PP_0761 at 876.621 kb on + strand, within PP_0761 at 876.650 kb on - strand, within PP_0761 at 876.863 kb on + strand, within PP_0761 at 876.864 kb on - strand, within PP_0761 at 876.908 kb on + strand, within PP_0761 at 877.192 kb on + strand at 877.192 kb on + strand at 877.192 kb on + strand at 877.527 kb on + strand, within PP_0762 at 877.528 kb on - strand, within PP_0762 at 877.528 kb on - strand, within PP_0762 at 877.645 kb on + strand, within PP_0762 at 877.646 kb on - strand, within PP_0762 at 877.740 kb on + strand, within PP_0762 at 877.805 kb on + strand, within PP_0762 at 877.806 kb on - strand, within PP_0762 at 877.849 kb on - strand, within PP_0762 at 877.849 kb on - strand at 877.849 kb on - strand, within PP_0762 at 878.332 kb on - strand at 878.332 kb on - strand at 878.368 kb on + strand at 878.368 kb on + strand at 878.368 kb on + strand at 878.368 kb on + strand at 878.368 kb on + strand at 878.368 kb on + strand at 878.369 kb on - strand at 878.369 kb on - strand at 878.369 kb on - strand at 878.369 kb on - strand at 878.369 kb on - strand at 878.369 kb on - strand at 878.369 kb on - strand at 878.369 kb on - strand at 878.369 kb on - strand at 878.429 kb on - strand at 878.512 kb on - strand at 878.512 kb on - strand at 878.518 kb on - strand at 878.583 kb on + strand at 878.607 kb on + strand at 878.607 kb on + strand at 878.608 kb on - strand at 878.608 kb on - strand at 878.856 kb on + strand, within PP_0763 at 878.955 kb on + strand, within PP_0763 at 878.956 kb on - strand, within PP_0763 at 878.956 kb on - strand, within PP_0763 at 878.988 kb on + strand, within PP_0763 at 879.027 kb on - strand, within PP_0763 at 879.027 kb on - strand, within PP_0763 at 879.033 kb on + strand, within PP_0763 at 879.033 kb on + strand, within PP_0763 at 879.034 kb on - strand, within PP_0763 at 879.034 kb on - strand, within PP_0763 at 879.034 kb on - strand, within PP_0763 at 879.129 kb on - strand, within PP_0763 at 879.165 kb on + strand, within PP_0763 at 879.165 kb on + strand, within PP_0763 at 879.165 kb on + strand, within PP_0763 at 879.165 kb on + strand, within PP_0763 at 879.165 kb on + strand, within PP_0763 at 879.165 kb on + strand, within PP_0763 at 879.166 kb on - strand, within PP_0763
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. remove 876,338 + PP_0761 0.18 +0.0 876,346 - PP_0761 0.19 -0.3 876,379 + PP_0761 0.22 +0.9 876,379 + PP_0761 0.22 -0.4 876,379 + PP_0761 0.22 -0.9 876,379 + PP_0761 0.22 -0.5 876,380 - PP_0761 0.22 +0.7 876,381 + PP_0761 0.22 -1.5 876,382 - PP_0761 0.22 +0.4 876,382 - PP_0761 0.22 +1.7 876,382 - PP_0761 0.22 +0.6 876,621 + PP_0761 0.46 -1.8 876,621 + PP_0761 0.46 +1.1 876,650 - PP_0761 0.49 -0.1 876,863 + PP_0761 0.70 +0.4 876,864 - PP_0761 0.70 -0.7 876,908 + PP_0761 0.75 -1.4 877,192 + +0.4 877,192 + +0.7 877,192 + -0.1 877,527 + PP_0762 0.33 -0.8 877,528 - PP_0762 0.33 -0.0 877,528 - PP_0762 0.33 -2.7 877,645 + PP_0762 0.45 -1.3 877,646 - PP_0762 0.45 -2.8 877,740 + PP_0762 0.55 -1.2 877,805 + PP_0762 0.61 -1.4 877,806 - PP_0762 0.61 +0.6 877,849 - PP_0762 0.66 +1.1 877,849 - +0.7 877,849 - PP_0762 0.66 -1.0 878,332 - -0.4 878,332 - +2.7 878,368 + -0.7 878,368 + +0.3 878,368 + -1.0 878,368 + +1.7 878,368 + +2.3 878,368 + -1.7 878,369 - -0.1 878,369 - +0.3 878,369 - -1.2 878,369 - -0.5 878,369 - -0.6 878,369 - +1.1 878,369 - -0.6 878,369 - -0.1 878,369 - +0.5 878,429 - -2.2 878,512 - -0.8 878,512 - +0.7 878,518 - -0.8 878,583 + -0.3 878,607 + +0.4 878,607 + -2.0 878,608 - +0.1 878,608 - +0.4 878,856 + PP_0763 0.23 +0.7 878,955 + PP_0763 0.29 -0.9 878,956 - PP_0763 0.29 -0.1 878,956 - PP_0763 0.29 -0.1 878,988 + PP_0763 0.31 -0.9 879,027 - PP_0763 0.33 +0.8 879,027 - PP_0763 0.33 +0.4 879,033 + PP_0763 0.33 -0.1 879,033 + PP_0763 0.33 +0.7 879,034 - PP_0763 0.33 -0.9 879,034 - PP_0763 0.33 -0.2 879,034 - PP_0763 0.33 -1.1 879,129 - PP_0763 0.39 -1.0 879,165 + PP_0763 0.41 -0.7 879,165 + PP_0763 0.41 -0.7 879,165 + PP_0763 0.41 -1.0 879,165 + PP_0763 0.41 +1.7 879,165 + PP_0763 0.41 -0.1 879,165 + PP_0763 0.41 +0.4 879,166 - PP_0763 0.41 -0.2
Or see this region's nucleotide sequence