Strain Fitness in Pseudomonas putida KT2440 around PP_0569

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
660,657	-		PP_0567	0.89	+0.3
660,728	+				+0.3
660,728	+				+0.8
660,728	+				-1.4
660,815	+				-0.4
660,815	+				+0.6
660,816	-				-0.3
660,854	+				-0.4
660,854	+				+0.8
660,854	+				+1.7
660,855	-				-0.2
660,855	-				-0.8
660,855	-				-2.0
660,932	-				-0.6
660,966	+				+0.8
660,970	+				+1.0
661,003	-				-1.5
661,034	+				-1.2
661,060	+				-1.2
661,096	-		PP_0568	0.13	+1.7
661,247	+		PP_0568	0.46	+0.2
661,317	+		PP_0568	0.62	+0.5
661,317	+		PP_0568	0.62	-0.8
661,318	-		PP_0568	0.62	+0.8
661,318	-		PP_0568	0.62	+0.3
661,343	+		PP_0568	0.68	-0.7
661,343	+		PP_0568	0.68	-0.3
661,343	+		PP_0568	0.68	-0.3
661,343	+		PP_0568	0.68	+0.1
661,344	-		PP_0568	0.68	+0.3
661,344	-		PP_0568	0.68	+0.3
661,410	+		PP_0568	0.82	-0.2
661,410	+		PP_0568	0.82	-0.2
661,411	-		PP_0568	0.82	-1.3
661,461	+				-0.3
661,480	+				-1.2
661,480	+				+0.6
661,480	+				+0.5
661,480	+				-0.3
661,481	-				+1.3
661,481	-				-0.5
661,481	-				-0.3
661,481	-				-0.8
661,481	-				-0.3
661,834	+		PP_0569	0.16	-0.4
661,886	-		PP_0569	0.20	-0.9
661,886	-		PP_0569	0.20	-0.2
661,886	-		PP_0569	0.20	-0.2
662,092	+		PP_0569	0.36	-1.2
662,092	+		PP_0569	0.36	-2.2
662,093	-		PP_0569	0.36	+1.7
662,093	-		PP_0569	0.36	-1.0
662,093	-		PP_0569	0.36	+0.3
662,172	-		PP_0569	0.41	-1.7
662,204	+		PP_0569	0.44	-0.8
662,291	-		PP_0569	0.50	-1.1
662,406	+		PP_0569	0.59	+1.1
662,406	+		PP_0569	0.59	-0.7
662,406	+		PP_0569	0.59	-0.3
662,407	-		PP_0569	0.59	-0.9
662,407	-		PP_0569	0.59	-1.6
662,500	+		PP_0569	0.66	+0.1
663,012	+				-0.3
663,012	+				+1.1
663,012	+				-0.9
663,032	-				+0.5
663,089	+				-2.0
663,089	+				+0.1
663,090	-				-0.1
663,090	-				-0.2
663,090	-				-1.2
663,091	+				-0.1
663,091	+				-1.4
663,092	-				+0.3
663,092	-				-2.4
663,092	-				+2.7
663,092	-				-0.7
663,092	-				-0.0
663,108	+				+0.1
663,129	+				+1.0
663,129	+				-0.9
663,130	-				+0.4
663,230	+				-0.4
663,230	+				+0.5
663,230	+				-0.0
663,230	+				-1.4
663,231	-				+0.7
663,775	-		PP_0570	0.13	+2.7
663,775	-		PP_0570	0.13	-0.9
663,777	+		PP_0570	0.13	+1.3
663,777	+		PP_0570	0.13	+1.2
663,777	+		PP_0570	0.13	-2.1
663,777	+		PP_0570	0.13	+0.4
663,778	-		PP_0570	0.13	-1.0
663,778	-		PP_0570	0.13	+0.1
663,778	-		PP_0570	0.13	-0.3
663,778	-		PP_0570	0.13	-0.7
663,780	+		PP_0570	0.13	-1.2
663,780	+		PP_0570	0.13	-1.5
663,915	+		PP_0570	0.16	-0.1
663,945	+		PP_0570	0.17	+1.7
663,945	+		PP_0570	0.17	-1.7
663,946	-		PP_0570	0.17	-0.3