Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0501 and PP_0502 are separated by 30 nucleotides PP_0502 and PP_0503 are separated by 102 nucleotides PP_0503 and PP_0504 overlap by 13 nucleotides PP_0504 and PP_0505 are separated by 109 nucleotides
PP_0501: PP_0501 - NAD-dependent epimerase/dehydratase family protein, at 590,754 to 591,686
_0501
PP_0502: PP_0502 - conserved protein of unknown function, at 591,717 to 592,160
_0502
PP_0503: PP_0503 - Major facilitator family transporter, at 592,263 to 593,468
_0503
PP_0504: PP_0504 - Outer membrane protein OprG, at 593,456 to 594,139
_0504
PP_0505: PP_0505 - conserved protein of unknown function, at 594,249 to 594,761
_0505
Position (kb) 592
593
594 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 591.759 kb on + strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.759 kb on + strand at 591.760 kb on - strand at 591.760 kb on - strand at 591.760 kb on - strand at 591.760 kb on - strand at 591.793 kb on - strand, within PP_0502 at 591.928 kb on + strand, within PP_0502 at 591.929 kb on - strand, within PP_0502 at 591.936 kb on - strand, within PP_0502 at 591.937 kb on + strand, within PP_0502 at 591.937 kb on + strand, within PP_0502 at 591.938 kb on - strand, within PP_0502 at 591.997 kb on - strand, within PP_0502 at 592.111 kb on - strand, within PP_0502 at 592.111 kb on - strand, within PP_0502 at 592.211 kb on - strand at 592.214 kb on + strand at 592.215 kb on - strand at 592.289 kb on - strand at 592.335 kb on + strand at 592.335 kb on + strand at 592.336 kb on - strand at 592.336 kb on - strand at 592.429 kb on - strand, within PP_0503 at 592.555 kb on + strand, within PP_0503 at 592.555 kb on + strand, within PP_0503 at 592.556 kb on - strand, within PP_0503 at 592.556 kb on - strand, within PP_0503 at 592.556 kb on - strand, within PP_0503 at 592.752 kb on + strand, within PP_0503 at 593.090 kb on + strand, within PP_0503 at 593.091 kb on - strand, within PP_0503 at 593.091 kb on - strand, within PP_0503 at 593.277 kb on + strand, within PP_0503 at 593.277 kb on + strand, within PP_0503 at 593.278 kb on - strand, within PP_0503 at 593.278 kb on - strand, within PP_0503 at 593.278 kb on - strand, within PP_0503 at 593.289 kb on + strand, within PP_0503 at 593.289 kb on + strand, within PP_0503 at 593.400 kb on + strand at 593.400 kb on + strand at 593.401 kb on - strand at 593.466 kb on + strand at 593.484 kb on + strand at 593.484 kb on + strand at 593.484 kb on + strand at 593.484 kb on + strand at 593.484 kb on + strand at 593.484 kb on + strand at 593.485 kb on - strand at 593.485 kb on - strand at 593.485 kb on - strand at 593.485 kb on - strand at 593.485 kb on - strand at 593.485 kb on - strand at 593.485 kb on - strand at 593.485 kb on - strand at 593.486 kb on + strand at 593.486 kb on + strand at 593.526 kb on + strand, within PP_0504 at 593.526 kb on + strand, within PP_0504 at 593.527 kb on - strand, within PP_0504 at 593.577 kb on + strand, within PP_0504 at 593.578 kb on - strand, within PP_0504 at 593.620 kb on - strand, within PP_0504 at 593.620 kb on - strand, within PP_0504 at 593.620 kb on - strand, within PP_0504 at 593.644 kb on + strand, within PP_0504 at 593.648 kb on + strand, within PP_0504 at 593.649 kb on - strand, within PP_0504 at 593.727 kb on + strand, within PP_0504 at 593.728 kb on - strand, within PP_0504 at 593.728 kb on - strand, within PP_0504 at 593.781 kb on + strand, within PP_0504 at 593.781 kb on + strand, within PP_0504 at 593.884 kb on + strand, within PP_0504 at 593.925 kb on + strand, within PP_0504 at 593.926 kb on - strand, within PP_0504 at 593.926 kb on - strand, within PP_0504 at 593.926 kb on - strand, within PP_0504 at 593.926 kb on - strand at 594.072 kb on + strand at 594.181 kb on + strand at 594.262 kb on + strand at 594.263 kb on - strand at 594.263 kb on - strand at 594.263 kb on - strand at 594.352 kb on + strand, within PP_0505 at 594.361 kb on + strand, within PP_0505 at 594.362 kb on - strand, within PP_0505 at 594.448 kb on + strand, within PP_0505 at 594.448 kb on + strand, within PP_0505 at 594.449 kb on - strand, within PP_0505 at 594.449 kb on - strand, within PP_0505 at 594.449 kb on - strand, within PP_0505 at 594.451 kb on + strand, within PP_0505 at 594.452 kb on - strand at 594.452 kb on - strand, within PP_0505 at 594.452 kb on - strand, within PP_0505
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. remove 591,759 + +0.2 591,759 + -0.1 591,759 + +1.6 591,759 + -1.5 591,759 + -1.1 591,760 - -0.4 591,760 - +1.4 591,760 - -1.6 591,760 - +0.1 591,793 - PP_0502 0.17 -0.6 591,928 + PP_0502 0.48 -0.2 591,929 - PP_0502 0.48 +0.2 591,936 - PP_0502 0.49 +1.6 591,937 + PP_0502 0.50 -0.2 591,937 + PP_0502 0.50 +1.0 591,938 - PP_0502 0.50 +0.6 591,997 - PP_0502 0.63 -1.4 592,111 - PP_0502 0.89 +0.5 592,111 - PP_0502 0.89 +0.5 592,211 - -0.2 592,214 + -1.6 592,215 - +1.2 592,289 - -0.5 592,335 + +1.6 592,335 + +2.2 592,336 - +0.5 592,336 - +2.6 592,429 - PP_0503 0.14 +0.6 592,555 + PP_0503 0.24 +0.8 592,555 + PP_0503 0.24 +0.6 592,556 - PP_0503 0.24 +0.5 592,556 - PP_0503 0.24 +1.0 592,556 - PP_0503 0.24 +0.6 592,752 + PP_0503 0.41 -0.4 593,090 + PP_0503 0.69 +1.6 593,091 - PP_0503 0.69 +1.1 593,091 - PP_0503 0.69 -0.7 593,277 + PP_0503 0.84 +0.8 593,277 + PP_0503 0.84 +0.8 593,278 - PP_0503 0.84 -0.2 593,278 - PP_0503 0.84 +0.4 593,278 - PP_0503 0.84 +2.6 593,289 + PP_0503 0.85 +2.6 593,289 + PP_0503 0.85 +0.3 593,400 + -1.4 593,400 + -1.7 593,401 - +0.4 593,466 + +2.6 593,484 + +2.6 593,484 + +0.3 593,484 + +0.0 593,484 + -0.6 593,484 + +0.3 593,484 + +0.3 593,485 - -0.4 593,485 - +2.6 593,485 - +0.3 593,485 - +2.0 593,485 - -0.4 593,485 - -1.1 593,485 - -1.8 593,485 - -0.5 593,486 + +2.6 593,486 + +3.6 593,526 + PP_0504 0.10 -0.8 593,526 + PP_0504 0.10 -1.0 593,527 - PP_0504 0.10 -0.1 593,577 + PP_0504 0.18 -0.0 593,578 - PP_0504 0.18 +2.6 593,620 - PP_0504 0.24 -0.8 593,620 - PP_0504 0.24 -0.4 593,620 - PP_0504 0.24 +0.0 593,644 + PP_0504 0.27 +0.3 593,648 + PP_0504 0.28 -0.5 593,649 - PP_0504 0.28 +2.0 593,727 + PP_0504 0.40 +0.3 593,728 - PP_0504 0.40 +0.8 593,728 - PP_0504 0.40 +0.6 593,781 + PP_0504 0.48 +2.6 593,781 + PP_0504 0.48 +0.5 593,884 + PP_0504 0.63 +0.3 593,925 + PP_0504 0.69 -1.0 593,926 - PP_0504 0.69 +0.0 593,926 - PP_0504 0.69 +2.6 593,926 - PP_0504 0.69 -0.8 593,926 - +0.6 594,072 + -0.7 594,181 + +0.1 594,262 + -1.5 594,263 - +1.6 594,263 - +2.6 594,263 - -2.0 594,352 + PP_0505 0.20 +0.1 594,361 + PP_0505 0.22 +0.6 594,362 - PP_0505 0.22 +0.6 594,448 + PP_0505 0.39 +0.5 594,448 + PP_0505 0.39 -1.9 594,449 - PP_0505 0.39 +0.3 594,449 - PP_0505 0.39 -0.4 594,449 - PP_0505 0.39 +1.0 594,451 + PP_0505 0.39 +0.4 594,452 - +1.6 594,452 - PP_0505 0.40 -0.5 594,452 - PP_0505 0.40 +1.0
Or see this region's nucleotide sequence