Strain Fitness in Pseudomonas putida KT2440 around PP_0271

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0270 and PP_0271 are separated by 1 nucleotidesPP_0271 and PP_0272 are separated by 140 nucleotides PP_0270: PP_0270 - Sensor histidine kinase, at 327,882 to 329,249 _0270 PP_0271: PP_0271 - DNA-binding response regulator, at 329,251 to 329,970 _0271 PP_0272: PP_0272 - Outer membrane ferric siderophore receptor, at 330,111 to 332,408 _0272 Position (kb) 329 330Strain fitness (log2 ratio) -2 -1 0 1 2 3at 328.358 kb on + strandat 328.358 kb on + strand, within PP_0270at 328.358 kb on + strand, within PP_0270at 328.358 kb on + strand, within PP_0270at 328.358 kb on + strand, within PP_0270at 328.358 kb on + strand, within PP_0270at 328.358 kb on + strand, within PP_0270at 328.358 kb on + strand, within PP_0270at 328.359 kb on - strand, within PP_0270at 328.359 kb on - strand, within PP_0270at 328.359 kb on - strand, within PP_0270at 328.359 kb on - strand, within PP_0270at 328.483 kb on + strand, within PP_0270at 328.483 kb on + strand, within PP_0270at 328.484 kb on - strand, within PP_0270at 328.484 kb on - strand, within PP_0270at 328.699 kb on + strand, within PP_0270at 328.700 kb on - strand, within PP_0270at 328.700 kb on - strand, within PP_0270at 328.715 kb on - strand, within PP_0270at 328.817 kb on + strand, within PP_0270at 328.905 kb on + strand, within PP_0270at 328.905 kb on + strand, within PP_0270at 328.906 kb on - strand, within PP_0270at 328.906 kb on - strand, within PP_0270at 328.906 kb on - strand, within PP_0270at 328.930 kb on + strand, within PP_0270at 328.931 kb on - strand, within PP_0270at 329.027 kb on - strand, within PP_0270at 329.027 kb on - strand, within PP_0270at 329.049 kb on - strand, within PP_0270at 329.107 kb on - strand, within PP_0270at 329.118 kb on + strandat 329.288 kb on + strandat 329.288 kb on + strandat 329.288 kb on + strandat 329.289 kb on - strandat 329.480 kb on + strand, within PP_0271at 329.580 kb on + strand, within PP_0271at 329.580 kb on + strand, within PP_0271at 329.580 kb on + strand, within PP_0271at 329.580 kb on + strand, within PP_0271at 329.580 kb on + strand, within PP_0271at 329.581 kb on - strand, within PP_0271at 329.623 kb on + strand, within PP_0271at 329.624 kb on - strand, within PP_0271at 329.676 kb on - strand, within PP_0271at 329.784 kb on + strand, within PP_0271at 329.785 kb on - strand, within PP_0271at 329.910 kb on - strandat 329.910 kb on - strandat 330.011 kb on + strandat 330.012 kb on - strandat 330.012 kb on - strandat 330.022 kb on - strandat 330.022 kb on - strandat 330.022 kb on - strandat 330.022 kb on - strandat 330.031 kb on + strandat 330.031 kb on + strandat 330.031 kb on + strandat 330.031 kb on + strandat 330.031 kb on + strandat 330.031 kb on + strandat 330.032 kb on - strandat 330.032 kb on - strandat 330.032 kb on - strandat 330.032 kb on - strandat 330.032 kb on - strandat 330.274 kb on + strandat 330.306 kb on + strandat 330.307 kb on - strandat 330.308 kb on + strandat 330.308 kb on + strandat 330.309 kb on - strandat 330.505 kb on - strand, within PP_0272at 330.594 kb on + strand, within PP_0272at 330.711 kb on + strand, within PP_0272at 330.712 kb on - strand, within PP_0272at 330.720 kb on + strand, within PP_0272at 330.721 kb on - strand, within PP_0272at 330.721 kb on - strand, within PP_0272at 330.721 kb on - strand, within PP_0272at 330.721 kb on - strand, within PP_0272at 330.721 kb on - strand, within PP_0272at 330.921 kb on + strand, within PP_0272at 330.922 kb on - strand, within PP_0272at 330.922 kb on - strand, within PP_0272at 330.927 kb on + strand, within PP_0272at 330.927 kb on + strand, within PP_0272at 330.927 kb on + strand, within PP_0272at 330.927 kb on + strand, within PP_0272at 330.927 kb on + strand, within PP_0272at 330.928 kb on - strand, within PP_0272

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Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
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328,358 + +3.6
328,358 + PP_0270 0.35 +0.2
328,358 + PP_0270 0.35 -0.7
328,358 + PP_0270 0.35 +1.1
328,358 + PP_0270 0.35 +0.8
328,358 + PP_0270 0.35 -0.6
328,358 + PP_0270 0.35 -1.0
328,358 + PP_0270 0.35 +1.6
328,359 - PP_0270 0.35 +2.6
328,359 - PP_0270 0.35 +2.6
328,359 - PP_0270 0.35 -0.6
328,359 - PP_0270 0.35 -1.7
328,483 + PP_0270 0.44 +0.3
328,483 + PP_0270 0.44 +1.0
328,484 - PP_0270 0.44 -0.1
328,484 - PP_0270 0.44 -1.0
328,699 + PP_0270 0.60 +0.7
328,700 - PP_0270 0.60 -0.7
328,700 - PP_0270 0.60 -0.0
328,715 - PP_0270 0.61 -1.7
328,817 + PP_0270 0.68 +1.6
328,905 + PP_0270 0.75 -2.2
328,905 + PP_0270 0.75 -0.2
328,906 - PP_0270 0.75 -0.4
328,906 - PP_0270 0.75 -0.1
328,906 - PP_0270 0.75 +0.2
328,930 + PP_0270 0.77 +0.6
328,931 - PP_0270 0.77 +0.4
329,027 - PP_0270 0.84 -0.0
329,027 - PP_0270 0.84 +1.4
329,049 - PP_0270 0.85 +0.3
329,107 - PP_0270 0.90 -0.6
329,118 + -0.9
329,288 + +0.9
329,288 + -2.2
329,288 + -0.7
329,289 - +1.6
329,480 + PP_0271 0.32 -1.3
329,580 + PP_0271 0.46 -1.7
329,580 + PP_0271 0.46 -0.6
329,580 + PP_0271 0.46 +3.6
329,580 + PP_0271 0.46 -0.5
329,580 + PP_0271 0.46 +1.8
329,581 - PP_0271 0.46 -1.1
329,623 + PP_0271 0.52 -0.5
329,624 - PP_0271 0.52 -2.0
329,676 - PP_0271 0.59 -0.1
329,784 + PP_0271 0.74 -2.4
329,785 - PP_0271 0.74 +1.6
329,910 - +0.2
329,910 - +2.6
330,011 + -1.9
330,012 - +0.8
330,012 - +1.1
330,022 - -1.2
330,022 - -0.6
330,022 - +0.7
330,022 - +2.6
330,031 + -0.0
330,031 + -0.6
330,031 + -0.3
330,031 + -0.0
330,031 + -0.7
330,031 + -0.7
330,032 - +0.0
330,032 - -1.1
330,032 - +0.1
330,032 - -0.3
330,032 - +1.0
330,274 + -1.0
330,306 + -0.1
330,307 - +0.4
330,308 + -0.3
330,308 + +0.2
330,309 - +1.0
330,505 - PP_0272 0.17 +0.8
330,594 + PP_0272 0.21 +0.6
330,711 + PP_0272 0.26 -0.6
330,712 - PP_0272 0.26 +0.6
330,720 + PP_0272 0.27 -0.0
330,721 - PP_0272 0.27 +3.6
330,721 - PP_0272 0.27 -0.6
330,721 - PP_0272 0.27 +1.0
330,721 - PP_0272 0.27 +1.1
330,721 - PP_0272 0.27 -0.4
330,921 + PP_0272 0.35 -1.7
330,922 - PP_0272 0.35 +1.0
330,922 - PP_0272 0.35 -0.5
330,927 + PP_0272 0.36 +1.0
330,927 + PP_0272 0.36 -0.3
330,927 + PP_0272 0.36 +1.6
330,927 + PP_0272 0.36 -1.9
330,927 + PP_0272 0.36 -2.6
330,928 - PP_0272 0.36 +0.2

Or see this region's nucleotide sequence