Strain Fitness in Pseudomonas putida KT2440 around PP_0203

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_5433 and PP_0202 are separated by 6 nucleotidesPP_0202 and PP_0203 are separated by 170 nucleotidesPP_0203 and PP_t74 are separated by 162 nucleotidesPP_t74 and PP_0204 are separated by 213 nucleotides PP_5433: PP_5433 - putative NADH:ubiquinone oxidoreductase subunit 2, at 252,133 to 252,771 _5433 PP_0202: PP_0202 - CBS domain-containing protein, at 252,778 to 253,215 _0202 PP_0203: PP_0203 - putative dipeptidase, at 253,386 to 255,116 _0203 PP_t74: PP_t74 - tRNA-Arg, at 255,279 to 255,355 _t74 PP_0204: PP_0204 - Transcriptional regulator, GntR family, at 255,569 to 256,318 _0204 Position (kb) 253 254 255 256Strain fitness (log2 ratio) -2 -1 0 1 2at 252.419 kb on - strand, within PP_5433at 252.433 kb on + strand, within PP_5433at 252.433 kb on + strand, within PP_5433at 252.433 kb on + strand, within PP_5433at 252.433 kb on + strand, within PP_5433at 252.434 kb on - strand, within PP_5433at 252.622 kb on + strand, within PP_5433at 252.623 kb on - strand, within PP_5433at 252.623 kb on - strand, within PP_5433at 252.623 kb on - strand, within PP_5433at 252.666 kb on + strand, within PP_5433at 252.703 kb on + strand, within PP_5433at 252.703 kb on + strand, within PP_5433at 252.704 kb on - strand, within PP_5433at 252.704 kb on - strand, within PP_5433at 252.726 kb on + strandat 252.726 kb on + strandat 252.727 kb on - strandat 252.794 kb on + strandat 252.794 kb on + strandat 252.795 kb on - strandat 253.043 kb on + strand, within PP_0202at 253.043 kb on + strand, within PP_0202at 253.163 kb on + strand, within PP_0202at 253.164 kb on - strand, within PP_0202at 253.164 kb on - strand, within PP_0202at 253.164 kb on - strand, within PP_0202at 253.215 kb on + strandat 253.215 kb on + strandat 253.216 kb on - strandat 253.254 kb on - strandat 253.257 kb on + strandat 253.258 kb on - strandat 253.258 kb on - strandat 253.258 kb on - strandat 253.340 kb on - strandat 253.493 kb on - strandat 253.568 kb on + strand, within PP_0203at 253.883 kb on - strand, within PP_0203at 253.926 kb on - strand, within PP_0203at 253.982 kb on + strand, within PP_0203at 253.982 kb on + strand, within PP_0203at 253.982 kb on + strand, within PP_0203at 253.982 kb on + strand, within PP_0203at 254.110 kb on + strand, within PP_0203at 254.110 kb on + strandat 254.110 kb on + strand, within PP_0203at 254.111 kb on - strand, within PP_0203at 254.111 kb on - strand, within PP_0203at 254.335 kb on + strand, within PP_0203at 254.336 kb on - strand, within PP_0203at 254.362 kb on + strand, within PP_0203at 254.362 kb on + strand, within PP_0203at 254.363 kb on - strand, within PP_0203at 254.363 kb on - strand, within PP_0203at 254.363 kb on - strand, within PP_0203at 254.363 kb on - strand, within PP_0203at 254.365 kb on + strand, within PP_0203at 254.366 kb on - strand, within PP_0203at 254.366 kb on - strand, within PP_0203at 254.366 kb on - strand, within PP_0203at 254.366 kb on - strand, within PP_0203at 254.366 kb on - strand, within PP_0203at 254.370 kb on + strand, within PP_0203at 254.370 kb on + strand, within PP_0203at 254.464 kb on + strand, within PP_0203at 254.465 kb on - strand, within PP_0203at 254.513 kb on - strand, within PP_0203at 254.526 kb on + strand, within PP_0203at 254.526 kb on + strand, within PP_0203at 254.527 kb on - strand, within PP_0203at 254.558 kb on + strand, within PP_0203at 254.657 kb on - strand, within PP_0203at 254.657 kb on - strand, within PP_0203at 254.750 kb on - strand, within PP_0203at 254.824 kb on + strand, within PP_0203at 254.824 kb on + strand, within PP_0203at 254.838 kb on - strand, within PP_0203at 254.839 kb on + strand, within PP_0203at 254.840 kb on - strand, within PP_0203at 254.903 kb on - strand, within PP_0203at 254.903 kb on - strand, within PP_0203at 255.059 kb on + strandat 255.060 kb on - strandat 255.060 kb on - strandat 255.278 kb on - strandat 255.278 kb on - strandat 255.372 kb on - strandat 255.477 kb on - strandat 255.507 kb on + strandat 255.517 kb on - strandat 255.517 kb on - strandat 255.539 kb on + strandat 255.598 kb on - strandat 255.605 kb on + strandat 255.605 kb on + strandat 255.605 kb on + strandat 255.606 kb on - strandat 255.606 kb on - strandat 255.607 kb on + strandat 255.628 kb on - strandat 255.649 kb on + strand, within PP_0204at 255.653 kb on + strand, within PP_0204at 255.654 kb on - strand, within PP_0204at 255.654 kb on - strand, within PP_0204at 255.654 kb on - strand, within PP_0204at 255.664 kb on + strand, within PP_0204at 255.665 kb on - strand, within PP_0204at 255.665 kb on - strand, within PP_0204at 255.823 kb on + strand, within PP_0204at 255.946 kb on + strand, within PP_0204at 255.947 kb on - strand, within PP_0204at 255.947 kb on - strand, within PP_0204

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Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
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252,419 - PP_5433 0.45 +0.1
252,433 + PP_5433 0.47 +1.0
252,433 + PP_5433 0.47 +1.7
252,433 + PP_5433 0.47 -1.9
252,433 + PP_5433 0.47 -0.6
252,434 - PP_5433 0.47 -0.2
252,622 + PP_5433 0.77 -0.6
252,623 - PP_5433 0.77 -0.0
252,623 - PP_5433 0.77 -1.0
252,623 - PP_5433 0.77 +1.5
252,666 + PP_5433 0.83 +2.2
252,703 + PP_5433 0.89 -0.4
252,703 + PP_5433 0.89 +0.3
252,704 - PP_5433 0.89 -0.4
252,704 - PP_5433 0.89 -0.7
252,726 + -1.4
252,726 + +1.4
252,727 - +0.4
252,794 + +1.4
252,794 + -0.3
252,795 - -1.3
253,043 + PP_0202 0.61 -0.2
253,043 + PP_0202 0.61 -0.2
253,163 + PP_0202 0.88 +1.2
253,164 - PP_0202 0.88 -0.9
253,164 - PP_0202 0.88 +0.6
253,164 - PP_0202 0.88 +0.4
253,215 + +0.5
253,215 + -0.8
253,216 - -0.1
253,254 - -0.1
253,257 + -0.1
253,258 - +0.4
253,258 - -0.2
253,258 - +1.5
253,340 - -1.0
253,493 - -1.0
253,568 + PP_0203 0.11 -2.2
253,883 - PP_0203 0.29 +0.5
253,926 - PP_0203 0.31 -1.5
253,982 + PP_0203 0.34 +2.5
253,982 + PP_0203 0.34 +1.5
253,982 + PP_0203 0.34 +0.5
253,982 + PP_0203 0.34 -0.3
254,110 + PP_0203 0.42 -1.0
254,110 + +1.5
254,110 + PP_0203 0.42 +1.3
254,111 - PP_0203 0.42 +0.7
254,111 - PP_0203 0.42 +0.5
254,335 + PP_0203 0.55 -1.4
254,336 - PP_0203 0.55 +0.3
254,362 + PP_0203 0.56 +0.1
254,362 + PP_0203 0.56 -0.9
254,363 - PP_0203 0.56 +0.5
254,363 - PP_0203 0.56 +1.4
254,363 - PP_0203 0.56 -0.7
254,363 - PP_0203 0.56 -0.4
254,365 + PP_0203 0.57 +2.5
254,366 - PP_0203 0.57 -0.7
254,366 - PP_0203 0.57 +0.5
254,366 - PP_0203 0.57 -0.5
254,366 - PP_0203 0.57 -0.2
254,366 - PP_0203 0.57 +0.9
254,370 + PP_0203 0.57 -0.0
254,370 + PP_0203 0.57 -0.1
254,464 + PP_0203 0.62 -0.7
254,465 - PP_0203 0.62 +0.4
254,513 - PP_0203 0.65 -1.5
254,526 + PP_0203 0.66 -0.3
254,526 + PP_0203 0.66 -0.6
254,527 - PP_0203 0.66 +0.2
254,558 + PP_0203 0.68 +1.2
254,657 - PP_0203 0.73 +1.0
254,657 - PP_0203 0.73 +0.5
254,750 - PP_0203 0.79 -0.9
254,824 + PP_0203 0.83 +0.6
254,824 + PP_0203 0.83 +0.3
254,838 - PP_0203 0.84 +0.1
254,839 + PP_0203 0.84 +0.3
254,840 - PP_0203 0.84 +0.5
254,903 - PP_0203 0.88 +1.4
254,903 - PP_0203 0.88 +1.0
255,059 + +2.5
255,060 - +0.9
255,060 - +1.9
255,278 - -0.0
255,278 - +2.5
255,372 - -0.0
255,477 - +0.8
255,507 + +0.5
255,517 - +0.5
255,517 - -0.3
255,539 + -0.3
255,598 - -0.9
255,605 + +2.5
255,605 + +0.7
255,605 + -0.9
255,606 - -0.7
255,606 - +0.3
255,607 + +0.9
255,628 - -1.5
255,649 + PP_0204 0.11 +0.3
255,653 + PP_0204 0.11 +0.5
255,654 - PP_0204 0.11 -0.8
255,654 - PP_0204 0.11 +1.0
255,654 - PP_0204 0.11 +0.6
255,664 + PP_0204 0.13 -0.7
255,665 - PP_0204 0.13 +0.6
255,665 - PP_0204 0.13 +1.5
255,823 + PP_0204 0.34 -0.4
255,946 + PP_0204 0.50 -0.8
255,947 - PP_0204 0.50 +0.5
255,947 - PP_0204 0.50 -1.3

Or see this region's nucleotide sequence