Strain Fitness in Pseudomonas putida KT2440 around PP_0199

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0197 and PP_0198 are separated by 99 nucleotidesPP_0198 and PP_0199 are separated by 48 nucleotidesPP_0199 and PP_0200 are separated by 8 nucleotides PP_0197: PP_0197 - conserved protein of unknown function, at 247,733 to 248,320 _0197 PP_0198: PP_0198 - putative transporter, at 248,420 to 249,052 _0198 PP_0199: PP_0199 - putative membrane protease family, stomatin, at 249,101 to 249,847 _0199 PP_0200: PP_0200 - putative membrane bound peptidase, nefD, at 249,856 to 251,202 _0200 Position (kb) 249 250Strain fitness (log2 ratio) -2 -1 0 1 2at 248.165 kb on - strand, within PP_0197at 248.233 kb on + strand, within PP_0197at 248.233 kb on + strand, within PP_0197at 248.233 kb on + strand, within PP_0197at 248.233 kb on + strand, within PP_0197at 248.234 kb on - strand, within PP_0197at 248.234 kb on - strand, within PP_0197at 248.281 kb on - strandat 248.380 kb on - strandat 248.433 kb on + strandat 248.433 kb on + strandat 248.433 kb on + strandat 248.433 kb on + strandat 248.433 kb on + strandat 248.434 kb on - strandat 248.434 kb on - strandat 248.434 kb on - strandat 248.434 kb on - strandat 248.672 kb on + strand, within PP_0198at 248.681 kb on + strand, within PP_0198at 248.681 kb on + strand, within PP_0198at 248.682 kb on - strand, within PP_0198at 248.864 kb on + strand, within PP_0198at 248.864 kb on + strand, within PP_0198at 248.865 kb on - strand, within PP_0198at 248.865 kb on - strand, within PP_0198at 248.919 kb on - strand, within PP_0198at 249.062 kb on + strandat 249.063 kb on - strandat 249.102 kb on + strandat 249.102 kb on + strandat 249.103 kb on - strandat 249.137 kb on + strandat 249.199 kb on + strand, within PP_0199at 249.199 kb on + strandat 249.199 kb on + strand, within PP_0199at 249.200 kb on - strand, within PP_0199at 249.200 kb on - strand, within PP_0199at 249.200 kb on - strand, within PP_0199at 249.201 kb on + strand, within PP_0199at 249.201 kb on + strandat 249.202 kb on - strand, within PP_0199at 249.202 kb on - strand, within PP_0199at 249.202 kb on - strand, within PP_0199at 249.425 kb on + strand, within PP_0199at 249.425 kb on + strand, within PP_0199at 249.425 kb on + strand, within PP_0199at 249.647 kb on + strand, within PP_0199at 249.664 kb on + strand, within PP_0199at 249.664 kb on + strand, within PP_0199at 249.664 kb on + strandat 249.664 kb on + strand, within PP_0199at 249.665 kb on - strand, within PP_0199at 249.665 kb on - strand, within PP_0199at 249.665 kb on - strand, within PP_0199at 249.680 kb on + strand, within PP_0199at 249.681 kb on - strand, within PP_0199at 249.772 kb on - strand, within PP_0199at 249.925 kb on + strandat 249.925 kb on + strandat 250.341 kb on - strand, within PP_0200at 250.341 kb on - strand, within PP_0200at 250.341 kb on - strand, within PP_0200at 250.362 kb on + strand, within PP_0200at 250.362 kb on + strandat 250.363 kb on - strand, within PP_0200at 250.363 kb on - strand, within PP_0200at 250.451 kb on - strand, within PP_0200at 250.724 kb on + strand, within PP_0200at 250.785 kb on + strand, within PP_0200

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Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
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248,165 - PP_0197 0.73 +0.2
248,233 + PP_0197 0.85 +0.9
248,233 + PP_0197 0.85 -0.5
248,233 + PP_0197 0.85 -0.3
248,233 + PP_0197 0.85 -0.0
248,234 - PP_0197 0.85 -2.0
248,234 - PP_0197 0.85 -0.6
248,281 - -0.0
248,380 - -1.4
248,433 + -0.6
248,433 + +0.2
248,433 + +1.5
248,433 + -0.6
248,433 + +0.7
248,434 - +0.3
248,434 - -0.7
248,434 - +2.5
248,434 - +1.0
248,672 + PP_0198 0.40 +0.7
248,681 + PP_0198 0.41 -0.6
248,681 + PP_0198 0.41 +1.5
248,682 - PP_0198 0.41 +2.0
248,864 + PP_0198 0.70 +2.5
248,864 + PP_0198 0.70 +2.5
248,865 - PP_0198 0.70 -0.9
248,865 - PP_0198 0.70 -0.1
248,919 - PP_0198 0.79 +2.5
249,062 + -1.5
249,063 - +2.5
249,102 + -0.7
249,102 + -0.7
249,103 - +0.2
249,137 + -0.5
249,199 + PP_0199 0.13 -1.1
249,199 + +1.0
249,199 + PP_0199 0.13 -0.7
249,200 - PP_0199 0.13 -0.8
249,200 - PP_0199 0.13 +1.2
249,200 - PP_0199 0.13 +0.1
249,201 + PP_0199 0.13 -2.2
249,201 + +2.0
249,202 - PP_0199 0.14 -0.5
249,202 - PP_0199 0.14 -0.7
249,202 - PP_0199 0.14 -0.6
249,425 + PP_0199 0.43 -0.5
249,425 + PP_0199 0.43 +0.5
249,425 + PP_0199 0.43 -0.0
249,647 + PP_0199 0.73 +1.5
249,664 + PP_0199 0.75 +2.5
249,664 + PP_0199 0.75 -1.6
249,664 + +2.5
249,664 + PP_0199 0.75 +1.5
249,665 - PP_0199 0.76 +0.3
249,665 - PP_0199 0.76 +0.3
249,665 - PP_0199 0.76 -0.5
249,680 + PP_0199 0.78 -0.8
249,681 - PP_0199 0.78 -0.4
249,772 - PP_0199 0.90 +0.5
249,925 + -0.7
249,925 + +0.2
250,341 - PP_0200 0.36 +1.1
250,341 - PP_0200 0.36 +0.0
250,341 - PP_0200 0.36 -0.0
250,362 + PP_0200 0.38 +1.0
250,362 + +1.5
250,363 - PP_0200 0.38 +0.5
250,363 - PP_0200 0.38 -0.6
250,451 - PP_0200 0.44 -0.3
250,724 + PP_0200 0.64 -2.0
250,785 + PP_0200 0.69 +0.0

Or see this region's nucleotide sequence