Strain Fitness in Pseudomonas putida KT2440 around PP_0137

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
143,788	+		PP_0136	0.50	+0.7
143,788	+		PP_0136	0.50	-0.2
143,788	+		PP_0136	0.50	+3.1
143,788	+		PP_0136	0.50	+0.2
143,788	+		PP_0136	0.50	-0.9
143,789	-		PP_0136	0.51	+1.3
143,789	-		PP_0136	0.51	+0.1
143,789	-		PP_0136	0.51	+0.7
143,789	-		PP_0136	0.51	+0.5
143,789	-		PP_0136	0.51	+0.9
143,789	-		PP_0136	0.51	-0.5
143,831	-		PP_0136	0.59	+1.5
143,831	-		PP_0136	0.59	+0.5
143,831	-		PP_0136	0.59	+0.5
143,831	-		PP_0136	0.59	+1.1
143,875	+		PP_0136	0.69	+2.1
144,017	-				-1.3
144,064	+				+0.2
144,065	-				-1.5
144,065	-				-1.3
144,067	+				+2.5
144,067	+				-0.8
144,068	-				-0.2
144,068	-				+0.3
144,068	-				+1.1
144,085	-				+0.1
144,086	+				-0.5
144,086	+				-0.2
144,087	-				-1.3
144,099	+				-0.4
144,099	+				-0.7
144,099	+				+2.5
144,099	+				-2.4
144,099	+				-0.7
144,100	-				-1.4
144,100	-				+0.4
144,100	-				+1.5
144,160	+				-0.2
144,161	-				-1.5
144,178	+				+0.2
144,178	+				-0.6
144,179	-				-1.3
144,202	+				-0.8
144,203	-				-0.3
144,262	-				-0.7
144,294	+				+0.6
144,295	-				+0.5
144,295	-				-0.9
144,295	-				-0.7
144,295	-				-1.6
144,352	+				-1.9
144,352	+				-0.0
144,353	-				-0.5
144,386	+				+0.1
144,386	+				-0.5
144,386	+				-0.2
144,386	+				-2.1
144,387	-				+1.2
144,439	+				-0.7
144,439	+				-0.1
144,503	-				+0.2
144,545	-				-0.8
144,581	-				+0.1
144,605	+				+0.8
144,605	+				+0.2
144,605	+				-0.2
144,606	-				-0.1
144,606	-				+0.4
144,606	-				-2.9
144,660	+				-2.0
144,660	+				-0.9
144,660	+				+0.5
144,661	-				-0.5
144,661	-				-1.2
144,661	-				-0.3
144,661	-				-0.7
144,661	-				+0.2
144,661	-				+0.1
144,661	-				+1.5
144,661	-				+1.3
144,688	+				-1.6
144,688	-				+1.9
144,688	-				+0.3
144,688	-				-0.3
144,760	+				+0.9
144,760	+				+0.8
145,025	+		PP_0137	0.19	-2.2
145,025	+		PP_0137	0.19	+0.1
145,026	-		PP_0137	0.19	-1.0
145,026	-		PP_0137	0.19	-0.3
145,059	+		PP_0137	0.22	-0.3
145,059	+		PP_0137	0.22	+0.4
145,129	+		PP_0137	0.27	+0.4
145,129	+		PP_0137	0.27	-0.0
145,139	+		PP_0137	0.28	-0.9
145,139	+		PP_0137	0.28	-0.1
145,162	-		PP_0137	0.30	+2.5
145,162	-		PP_0137	0.30	-1.3
145,324	+		PP_0137	0.42	+0.2
145,380	-		PP_0137	0.46	-1.3
145,380	-		PP_0137	0.46	-0.1
145,380	-		PP_0137	0.46	-0.8
145,475	+		PP_0137	0.53	-0.8
145,475	+		PP_0137	0.53	-0.5
145,475	+		PP_0137	0.53	-0.7
145,476	-		PP_0137	0.53	-1.4
145,476	-		PP_0137	0.53	-0.9
145,506	+		PP_0137	0.56	+0.1
145,586	+		PP_0137	0.62	-0.2
145,586	+		PP_0137	0.62	+1.3
145,643	+		PP_0137	0.66	-0.9
145,701	+		PP_0137	0.70	-0.1
145,702	-		PP_0137	0.70	-0.1
145,718	+		PP_0137	0.72	+2.5
145,719	-		PP_0137	0.72	+0.3
145,754	+		PP_0137	0.74	+0.1
145,754	+		PP_0137	0.74	+0.3
145,842	+		PP_0137	0.81	+0.9
145,842	+		PP_0137	0.81	-2.9
145,943	-		PP_0137	0.88	-1.0
145,943	-		PP_0137	0.88	-0.2
146,006	+				+2.5
146,006	+				+0.3
146,006	+				+0.9
146,007	-				+0.1
146,007	-				-1.1
146,007	-				-0.7
146,030	+				+0.9
146,031	-				-0.2
146,031	-				+0.9
146,033	+				-0.5
146,034	-				-0.8
146,034	-				-1.5
146,067	+				-0.7
146,067	+				-2.4
146,204	+				+0.2
146,238	+				-0.7
146,238	+				-2.2
146,238	+				-0.5
146,238	+				+1.5
146,238	+				+1.9
146,239	-				+0.1
146,239	-				-1.1
146,239	-				-0.1
146,239	-				-0.7
146,239	-				-0.9
146,239	-				-0.7
146,239	-				-1.1
146,585	-		PP_0138	0.28	+0.0
146,721	-		PP_0138	0.39	+0.9
146,861	+		PP_0138	0.50	+0.4
146,861	+		PP_0138	0.50	-0.7
146,861	+		PP_0138	0.50	-0.5
146,862	-		PP_0138	0.50	-0.7
146,862	-				+1.5
146,862	-		PP_0138	0.50	-1.9
147,023	+		PP_0138	0.63	-1.4
147,023	+		PP_0138	0.63	-0.2
147,023	+		PP_0138	0.63	-0.2
147,023	+		PP_0138	0.63	+1.5
147,023	+		PP_0138	0.63	+0.2
147,023	+		PP_0138	0.63	-0.3
147,024	-		PP_0138	0.63	-0.9
147,024	-		PP_0138	0.63	-0.5
147,024	-		PP_0138	0.63	-0.2
147,024	-		PP_0138	0.63	-0.9
147,083	+		PP_0138	0.68	-0.8
147,083	+		PP_0138	0.68	+2.2