Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu6127 and Atu6128 overlap by 4 nucleotides Atu6128 and Atu6129 are separated by 88 nucleotides Atu6129 and Atu6130 are separated by 13 nucleotides
Atu6127: Atu6127 - OriT nicking enzyme, Dtr system, at 143,987 to 147,289
Atu6127
Atu6128: Atu6128 - Conjugative pilin processing protease, at 147,286 to 147,816
Atu6128
Atu6129: Atu6129 - conjugal transfer protein, at 147,905 to 149,002
Atu6129
Atu6130: Atu6130 - conserved hypothetical protein, at 149,016 to 149,645
Atu6130
Position (kb)
147
148
149
150 Strain fitness (log2 ratio)
-1
0
1
2
3 at 146.941 kb on + strand, within Atu6127 at 146.941 kb on + strand, within Atu6127 at 146.947 kb on - strand, within Atu6127 at 147.050 kb on - strand at 147.077 kb on + strand at 147.077 kb on + strand at 147.082 kb on + strand at 147.141 kb on + strand at 147.164 kb on + strand at 147.212 kb on - strand at 147.236 kb on - strand at 147.288 kb on + strand at 147.301 kb on + strand at 147.301 kb on + strand at 147.331 kb on + strand at 147.339 kb on - strand, within Atu6128 at 147.339 kb on - strand, within Atu6128 at 147.469 kb on + strand, within Atu6128 at 147.515 kb on + strand, within Atu6128 at 147.525 kb on + strand, within Atu6128 at 147.535 kb on - strand, within Atu6128 at 147.535 kb on - strand, within Atu6128 at 147.536 kb on + strand, within Atu6128 at 147.627 kb on + strand, within Atu6128 at 147.651 kb on + strand, within Atu6128 at 147.673 kb on + strand, within Atu6128 at 147.749 kb on + strand, within Atu6128 at 147.757 kb on - strand, within Atu6128 at 147.768 kb on - strand at 147.768 kb on - strand at 147.783 kb on - strand at 147.796 kb on + strand at 147.803 kb on + strand at 147.804 kb on - strand at 147.806 kb on - strand at 147.806 kb on - strand at 147.835 kb on + strand at 147.902 kb on + strand at 148.005 kb on - strand at 148.023 kb on - strand, within Atu6129 at 148.049 kb on - strand, within Atu6129 at 148.101 kb on - strand, within Atu6129 at 148.101 kb on - strand, within Atu6129 at 148.141 kb on + strand, within Atu6129 at 148.141 kb on + strand, within Atu6129 at 148.149 kb on - strand, within Atu6129 at 148.198 kb on - strand, within Atu6129 at 148.223 kb on + strand, within Atu6129 at 148.274 kb on + strand, within Atu6129 at 148.372 kb on + strand, within Atu6129 at 148.380 kb on - strand, within Atu6129 at 148.403 kb on + strand, within Atu6129 at 148.411 kb on - strand, within Atu6129 at 148.420 kb on + strand, within Atu6129 at 148.420 kb on + strand, within Atu6129 at 148.428 kb on - strand, within Atu6129 at 148.458 kb on + strand, within Atu6129 at 148.470 kb on + strand, within Atu6129 at 148.650 kb on - strand, within Atu6129 at 148.655 kb on + strand, within Atu6129 at 148.655 kb on + strand, within Atu6129 at 148.663 kb on - strand, within Atu6129 at 148.678 kb on + strand, within Atu6129 at 148.752 kb on - strand, within Atu6129 at 148.787 kb on + strand, within Atu6129 at 148.819 kb on - strand, within Atu6129 at 148.826 kb on + strand, within Atu6129 at 148.842 kb on + strand, within Atu6129 at 148.842 kb on + strand, within Atu6129 at 148.988 kb on + strand at 148.988 kb on + strand at 149.006 kb on - strand at 149.031 kb on - strand at 149.033 kb on + strand at 149.041 kb on - strand at 149.081 kb on + strand, within Atu6130 at 149.175 kb on - strand, within Atu6130 at 149.183 kb on + strand, within Atu6130 at 149.235 kb on - strand, within Atu6130 at 149.237 kb on + strand, within Atu6130 at 149.273 kb on + strand, within Atu6130 at 149.278 kb on + strand, within Atu6130 at 149.443 kb on + strand, within Atu6130 at 149.453 kb on + strand, within Atu6130 at 149.453 kb on + strand, within Atu6130 at 149.525 kb on + strand, within Atu6130 at 149.555 kb on + strand, within Atu6130 at 149.563 kb on - strand, within Atu6130 at 149.589 kb on - strand at 149.634 kb on + strand at 149.636 kb on + strand at 149.642 kb on - strand at 149.745 kb on - strand at 149.781 kb on + strand at 149.789 kb on - strand at 149.789 kb on - strand at 149.823 kb on + strand at 149.831 kb on - strand at 149.837 kb on - strand at 149.931 kb on + strand at 149.991 kb on + strand at 149.991 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X remove 146,941 + Atu6127 0.89 +1.7 146,941 + Atu6127 0.89 -0.4 146,947 - Atu6127 0.90 -1.1 147,050 - +1.7 147,077 + +1.1 147,077 + -1.5 147,082 + +0.2 147,141 + +0.5 147,164 + +0.4 147,212 - +1.3 147,236 - +1.3 147,288 + -0.0 147,301 + -0.7 147,301 + +0.1 147,331 + +0.9 147,339 - Atu6128 0.10 +1.7 147,339 - Atu6128 0.10 +0.3 147,469 + Atu6128 0.34 +0.5 147,515 + Atu6128 0.43 -0.3 147,525 + Atu6128 0.45 +1.2 147,535 - Atu6128 0.47 +0.3 147,535 - Atu6128 0.47 +0.7 147,536 + Atu6128 0.47 -0.2 147,627 + Atu6128 0.64 +1.4 147,651 + Atu6128 0.69 +1.3 147,673 + Atu6128 0.73 -0.8 147,749 + Atu6128 0.87 +0.8 147,757 - Atu6128 0.89 +1.1 147,768 - -1.0 147,768 - +0.9 147,783 - +1.3 147,796 + +0.7 147,803 + +1.6 147,804 - +1.3 147,806 - +1.6 147,806 - +1.7 147,835 + +0.7 147,902 + +0.3 148,005 - +1.3 148,023 - Atu6129 0.11 -1.3 148,049 - Atu6129 0.13 -0.9 148,101 - Atu6129 0.18 +1.7 148,101 - Atu6129 0.18 +1.3 148,141 + Atu6129 0.21 +0.1 148,141 + Atu6129 0.21 +0.9 148,149 - Atu6129 0.22 -0.4 148,198 - Atu6129 0.27 -1.0 148,223 + Atu6129 0.29 +0.5 148,274 + Atu6129 0.34 -0.2 148,372 + Atu6129 0.43 +0.5 148,380 - Atu6129 0.43 -0.2 148,403 + Atu6129 0.45 +2.1 148,411 - Atu6129 0.46 +0.9 148,420 + Atu6129 0.47 +0.3 148,420 + Atu6129 0.47 -1.4 148,428 - Atu6129 0.48 +1.3 148,458 + Atu6129 0.50 +1.1 148,470 + Atu6129 0.51 -0.1 148,650 - Atu6129 0.68 +3.3 148,655 + Atu6129 0.68 +0.4 148,655 + Atu6129 0.68 +0.3 148,663 - Atu6129 0.69 -0.2 148,678 + Atu6129 0.70 +0.5 148,752 - Atu6129 0.77 -0.8 148,787 + Atu6129 0.80 +0.9 148,819 - Atu6129 0.83 +0.3 148,826 + Atu6129 0.84 +1.7 148,842 + Atu6129 0.85 +0.1 148,842 + Atu6129 0.85 +0.7 148,988 + -1.5 148,988 + +0.3 149,006 - -0.9 149,031 - +0.8 149,033 + -0.9 149,041 - +1.7 149,081 + Atu6130 0.10 -0.4 149,175 - Atu6130 0.25 +0.8 149,183 + Atu6130 0.27 +0.4 149,235 - Atu6130 0.35 +0.3 149,237 + Atu6130 0.35 -1.5 149,273 + Atu6130 0.41 -0.6 149,278 + Atu6130 0.42 +0.4 149,443 + Atu6130 0.68 -0.2 149,453 + Atu6130 0.69 -0.2 149,453 + Atu6130 0.69 +0.1 149,525 + Atu6130 0.81 +0.4 149,555 + Atu6130 0.86 +2.0 149,563 - Atu6130 0.87 -0.4 149,589 - +0.1 149,634 + +0.8 149,636 + +0.4 149,642 - +0.7 149,745 - -1.7 149,781 + +0.8 149,789 - -0.3 149,789 - -0.2 149,823 + -0.8 149,831 - +0.3 149,837 - -0.3 149,931 + +1.0 149,991 + +1.0 149,991 + -0.0
Or see this region's nucleotide sequence