Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5457 and Atu5458 overlap by 4 nucleotides Atu5458 and Atu5459 are separated by 24 nucleotides
Atu5457: Atu5457 - dihydrodipicolinate synthase, at 449,411 to 450,319
Atu5457
Atu5458: Atu5458 - isomerase/lactonizing enzyme, at 450,316 to 451,482
Atu5458
Atu5459: Atu5459 - ABC transporter substrate binding protein (sugar), at 451,507 to 452,778
Atu5459
Position (kb)
450
451
452 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 449.436 kb on + strand at 449.439 kb on - strand at 449.439 kb on - strand at 449.444 kb on - strand at 449.495 kb on - strand at 449.515 kb on + strand, within Atu5457 at 449.522 kb on + strand, within Atu5457 at 449.530 kb on - strand, within Atu5457 at 449.652 kb on - strand, within Atu5457 at 449.672 kb on - strand, within Atu5457 at 449.682 kb on - strand, within Atu5457 at 449.754 kb on + strand, within Atu5457 at 449.754 kb on + strand, within Atu5457 at 449.754 kb on + strand, within Atu5457 at 449.762 kb on - strand, within Atu5457 at 449.826 kb on + strand, within Atu5457 at 449.826 kb on + strand, within Atu5457 at 449.834 kb on - strand, within Atu5457 at 449.834 kb on - strand, within Atu5457 at 449.841 kb on + strand, within Atu5457 at 449.841 kb on + strand, within Atu5457 at 450.052 kb on + strand, within Atu5457 at 450.122 kb on + strand, within Atu5457 at 450.214 kb on - strand, within Atu5457 at 450.335 kb on + strand at 450.344 kb on - strand at 450.385 kb on - strand at 450.417 kb on + strand at 450.419 kb on + strand at 450.419 kb on + strand at 450.427 kb on - strand at 450.446 kb on - strand, within Atu5458 at 450.456 kb on - strand, within Atu5458 at 450.456 kb on - strand, within Atu5458 at 450.458 kb on + strand, within Atu5458 at 450.472 kb on + strand, within Atu5458 at 450.488 kb on + strand, within Atu5458 at 450.513 kb on + strand, within Atu5458 at 450.514 kb on + strand, within Atu5458 at 450.558 kb on + strand, within Atu5458 at 450.566 kb on - strand, within Atu5458 at 450.605 kb on + strand, within Atu5458 at 450.651 kb on + strand, within Atu5458 at 450.659 kb on - strand, within Atu5458 at 450.713 kb on + strand, within Atu5458 at 450.772 kb on + strand, within Atu5458 at 450.778 kb on - strand, within Atu5458 at 450.793 kb on - strand, within Atu5458 at 450.803 kb on - strand, within Atu5458 at 450.851 kb on + strand, within Atu5458 at 450.955 kb on + strand, within Atu5458 at 450.963 kb on - strand, within Atu5458 at 451.006 kb on + strand, within Atu5458 at 451.021 kb on - strand, within Atu5458 at 451.021 kb on - strand, within Atu5458 at 451.041 kb on + strand, within Atu5458 at 451.188 kb on + strand, within Atu5458 at 451.194 kb on - strand, within Atu5458 at 451.255 kb on - strand, within Atu5458 at 451.285 kb on - strand, within Atu5458 at 451.329 kb on - strand, within Atu5458 at 451.410 kb on - strand at 451.444 kb on - strand at 451.444 kb on - strand at 451.452 kb on + strand at 451.531 kb on + strand at 451.587 kb on + strand at 451.595 kb on - strand at 451.637 kb on - strand, within Atu5459 at 451.798 kb on + strand, within Atu5459 at 451.823 kb on + strand, within Atu5459 at 451.826 kb on - strand, within Atu5459 at 451.831 kb on - strand, within Atu5459 at 451.905 kb on + strand, within Atu5459 at 451.906 kb on + strand, within Atu5459 at 451.907 kb on + strand, within Atu5459 at 451.913 kb on - strand, within Atu5459 at 451.951 kb on + strand, within Atu5459 at 452.022 kb on + strand, within Atu5459 at 452.070 kb on + strand, within Atu5459 at 452.070 kb on + strand, within Atu5459 at 452.089 kb on + strand, within Atu5459 at 452.159 kb on - strand, within Atu5459 at 452.200 kb on + strand, within Atu5459 at 452.208 kb on - strand, within Atu5459 at 452.217 kb on + strand, within Atu5459 at 452.259 kb on - strand, within Atu5459 at 452.318 kb on + strand, within Atu5459 at 452.326 kb on - strand, within Atu5459 at 452.327 kb on - strand, within Atu5459 at 452.367 kb on + strand, within Atu5459
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X remove 449,436 + -0.6 449,439 - -0.8 449,439 - +0.7 449,444 - +2.5 449,495 - +1.0 449,515 + Atu5457 0.11 +3.1 449,522 + Atu5457 0.12 +1.1 449,530 - Atu5457 0.13 +1.1 449,652 - Atu5457 0.27 -0.0 449,672 - Atu5457 0.29 +1.6 449,682 - Atu5457 0.30 -0.2 449,754 + Atu5457 0.38 -0.1 449,754 + Atu5457 0.38 +0.8 449,754 + Atu5457 0.38 -0.5 449,762 - Atu5457 0.39 +1.5 449,826 + Atu5457 0.46 +0.6 449,826 + Atu5457 0.46 -0.4 449,834 - Atu5457 0.47 -0.4 449,834 - Atu5457 0.47 +0.0 449,841 + Atu5457 0.47 +1.2 449,841 + Atu5457 0.47 -0.1 450,052 + Atu5457 0.71 +0.6 450,122 + Atu5457 0.78 +4.1 450,214 - Atu5457 0.88 +0.4 450,335 + +0.2 450,344 - +1.3 450,385 - +2.7 450,417 + -0.2 450,419 + +0.8 450,419 + +0.1 450,427 - +0.2 450,446 - Atu5458 0.11 +1.5 450,456 - Atu5458 0.12 +1.1 450,456 - Atu5458 0.12 +2.1 450,458 + Atu5458 0.12 +2.4 450,472 + Atu5458 0.13 +1.7 450,488 + Atu5458 0.15 -2.3 450,513 + Atu5458 0.17 +2.1 450,514 + Atu5458 0.17 +0.8 450,558 + Atu5458 0.21 -0.2 450,566 - Atu5458 0.21 -1.3 450,605 + Atu5458 0.25 +1.5 450,651 + Atu5458 0.29 -1.7 450,659 - Atu5458 0.29 +0.8 450,713 + Atu5458 0.34 +1.8 450,772 + Atu5458 0.39 -0.5 450,778 - Atu5458 0.40 +0.5 450,793 - Atu5458 0.41 -0.2 450,803 - Atu5458 0.42 +1.5 450,851 + Atu5458 0.46 +1.0 450,955 + Atu5458 0.55 -0.1 450,963 - Atu5458 0.55 +4.1 451,006 + Atu5458 0.59 -0.4 451,021 - Atu5458 0.60 -0.9 451,021 - Atu5458 0.60 +1.5 451,041 + Atu5458 0.62 -0.4 451,188 + Atu5458 0.75 +2.7 451,194 - Atu5458 0.75 +0.6 451,255 - Atu5458 0.80 -0.9 451,285 - Atu5458 0.83 +1.7 451,329 - Atu5458 0.87 +0.7 451,410 - +0.8 451,444 - -0.1 451,444 - +0.5 451,452 + +0.7 451,531 + -1.5 451,587 + +0.5 451,595 - +0.1 451,637 - Atu5459 0.10 +0.8 451,798 + Atu5459 0.23 +1.5 451,823 + Atu5459 0.25 +1.0 451,826 - Atu5459 0.25 +2.1 451,831 - Atu5459 0.25 -0.1 451,905 + Atu5459 0.31 +0.3 451,906 + Atu5459 0.31 +1.2 451,907 + Atu5459 0.31 +0.4 451,913 - Atu5459 0.32 -0.2 451,951 + Atu5459 0.35 +0.1 452,022 + Atu5459 0.40 +0.2 452,070 + Atu5459 0.44 +2.5 452,070 + Atu5459 0.44 +1.3 452,089 + Atu5459 0.46 -0.9 452,159 - Atu5459 0.51 +0.6 452,200 + Atu5459 0.54 +0.5 452,208 - Atu5459 0.55 +0.9 452,217 + Atu5459 0.56 +0.0 452,259 - Atu5459 0.59 +3.5 452,318 + Atu5459 0.64 -0.7 452,326 - Atu5459 0.64 +2.1 452,327 - Atu5459 0.64 +0.6 452,367 + Atu5459 0.68 -0.5
Or see this region's nucleotide sequence