Strain Fitness in Escherichia coli BW25113 around b4073

Experiment: LB Aerobic with Benzethonium chloride 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnrfB and nrfC overlap by 4 nucleotidesnrfC and nrfD overlap by 4 nucleotidesnrfD and nrfE are separated by 79 nucleotides b4071: nrfB - formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS), at 4,287,268 to 4,287,834 nrfB b4072: nrfC - formate-dependent nitrite reductase, 4Fe4S subunit (NCBI), at 4,287,831 to 4,288,502 nrfC b4073: nrfD - formate-dependent nitrite reductase, membrane subunit (NCBI), at 4,288,499 to 4,289,455 nrfD b4074: nrfE - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE (NCBI), at 4,289,535 to 4,291,193 nrfE Position (kb) 4288 4289 4290Strain fitness (log2 ratio) -2 -1 0 1 2at 4287.534 kb on - strand, within nrfBat 4287.560 kb on + strand, within nrfBat 4287.560 kb on + strand, within nrfBat 4287.582 kb on - strand, within nrfBat 4287.591 kb on + strand, within nrfBat 4287.619 kb on - strand, within nrfBat 4287.718 kb on + strand, within nrfBat 4287.823 kb on - strandat 4287.823 kb on - strandat 4287.836 kb on - strandat 4287.836 kb on - strandat 4287.905 kb on - strand, within nrfCat 4287.970 kb on - strand, within nrfCat 4287.981 kb on - strand, within nrfCat 4287.985 kb on + strand, within nrfCat 4288.125 kb on - strand, within nrfCat 4288.153 kb on - strand, within nrfCat 4288.182 kb on + strand, within nrfCat 4288.182 kb on + strand, within nrfCat 4288.212 kb on + strand, within nrfCat 4288.212 kb on + strand, within nrfCat 4288.220 kb on - strand, within nrfCat 4288.322 kb on + strand, within nrfCat 4288.458 kb on + strandat 4288.465 kb on + strandat 4288.465 kb on + strandat 4288.465 kb on + strandat 4288.465 kb on + strandat 4288.543 kb on - strandat 4288.617 kb on - strand, within nrfDat 4288.617 kb on - strand, within nrfDat 4288.650 kb on - strand, within nrfDat 4288.650 kb on - strand, within nrfDat 4288.655 kb on - strand, within nrfDat 4288.732 kb on + strand, within nrfDat 4288.740 kb on + strand, within nrfDat 4288.804 kb on + strand, within nrfDat 4288.804 kb on + strand, within nrfDat 4288.807 kb on + strand, within nrfDat 4288.825 kb on - strand, within nrfDat 4288.825 kb on - strand, within nrfDat 4288.888 kb on + strand, within nrfDat 4288.972 kb on + strand, within nrfDat 4288.972 kb on + strand, within nrfDat 4288.978 kb on + strand, within nrfDat 4288.978 kb on + strand, within nrfDat 4289.100 kb on - strand, within nrfDat 4289.154 kb on + strand, within nrfDat 4289.154 kb on + strandat 4289.163 kb on + strand, within nrfDat 4289.170 kb on + strand, within nrfDat 4289.170 kb on + strand, within nrfDat 4289.324 kb on + strand, within nrfDat 4289.324 kb on + strand, within nrfDat 4289.324 kb on + strand, within nrfDat 4289.361 kb on + strandat 4289.380 kb on + strandat 4289.543 kb on - strandat 4289.576 kb on - strandat 4289.621 kb on + strandat 4289.621 kb on + strandat 4289.647 kb on + strandat 4289.707 kb on + strand, within nrfEat 4289.707 kb on + strand, within nrfEat 4289.786 kb on + strand, within nrfEat 4289.896 kb on + strand, within nrfEat 4289.914 kb on - strand, within nrfEat 4289.914 kb on - strand, within nrfEat 4289.932 kb on + strand, within nrfEat 4289.932 kb on + strandat 4289.985 kb on + strand, within nrfEat 4289.985 kb on + strand, within nrfEat 4290.140 kb on + strand, within nrfEat 4290.255 kb on + strand, within nrfEat 4290.255 kb on + strand, within nrfEat 4290.265 kb on - strand, within nrfEat 4290.415 kb on - strand, within nrfEat 4290.415 kb on - strand, within nrfEat 4290.441 kb on - strand, within nrfEat 4290.443 kb on + strand, within nrfEat 4290.443 kb on + strand, within nrfE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Benzethonium chloride 0.000015625 mM
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4,287,534 - nrfB b4071 0.47 +0.3
4,287,560 + nrfB b4071 0.51 -0.4
4,287,560 + nrfB b4071 0.51 +0.2
4,287,582 - nrfB b4071 0.55 -0.9
4,287,591 + nrfB b4071 0.57 +0.2
4,287,619 - nrfB b4071 0.62 +0.3
4,287,718 + nrfB b4071 0.79 +0.1
4,287,823 - +0.2
4,287,823 - +0.1
4,287,836 - +1.5
4,287,836 - +0.0
4,287,905 - nrfC b4072 0.11 -0.1
4,287,970 - nrfC b4072 0.21 +1.0
4,287,981 - nrfC b4072 0.22 -0.4
4,287,985 + nrfC b4072 0.23 -0.6
4,288,125 - nrfC b4072 0.44 -0.4
4,288,153 - nrfC b4072 0.48 -0.5
4,288,182 + nrfC b4072 0.52 -0.3
4,288,182 + nrfC b4072 0.52 +0.8
4,288,212 + nrfC b4072 0.57 +0.5
4,288,212 + nrfC b4072 0.57 -0.5
4,288,220 - nrfC b4072 0.58 -0.1
4,288,322 + nrfC b4072 0.73 +0.6
4,288,458 + +0.2
4,288,465 + -0.9
4,288,465 + -0.7
4,288,465 + +0.2
4,288,465 + +0.4
4,288,543 - -0.0
4,288,617 - nrfD b4073 0.12 -0.5
4,288,617 - nrfD b4073 0.12 -0.1
4,288,650 - nrfD b4073 0.16 +0.3
4,288,650 - nrfD b4073 0.16 +0.6
4,288,655 - nrfD b4073 0.16 -0.4
4,288,732 + nrfD b4073 0.24 +1.2
4,288,740 + nrfD b4073 0.25 -0.1
4,288,804 + nrfD b4073 0.32 -1.8
4,288,804 + nrfD b4073 0.32 +0.1
4,288,807 + nrfD b4073 0.32 +0.4
4,288,825 - nrfD b4073 0.34 +1.8
4,288,825 - nrfD b4073 0.34 +0.8
4,288,888 + nrfD b4073 0.41 -0.1
4,288,972 + nrfD b4073 0.49 -0.1
4,288,972 + nrfD b4073 0.49 -0.2
4,288,978 + nrfD b4073 0.50 -0.0
4,288,978 + nrfD b4073 0.50 -0.7
4,289,100 - nrfD b4073 0.63 +0.0
4,289,154 + nrfD b4073 0.68 +0.1
4,289,154 + +0.2
4,289,163 + nrfD b4073 0.69 +0.4
4,289,170 + nrfD b4073 0.70 +0.6
4,289,170 + nrfD b4073 0.70 +0.4
4,289,324 + nrfD b4073 0.86 -1.8
4,289,324 + nrfD b4073 0.86 -0.1
4,289,324 + nrfD b4073 0.86 -0.2
4,289,361 + -1.4
4,289,380 + +0.5
4,289,543 - -1.9
4,289,576 - -0.9
4,289,621 + +0.9
4,289,621 + +1.4
4,289,647 + +0.4
4,289,707 + nrfE b4074 0.10 +0.9
4,289,707 + nrfE b4074 0.10 +1.6
4,289,786 + nrfE b4074 0.15 +0.1
4,289,896 + nrfE b4074 0.22 +1.0
4,289,914 - nrfE b4074 0.23 +0.1
4,289,914 - nrfE b4074 0.23 +1.6
4,289,932 + nrfE b4074 0.24 -0.5
4,289,932 + -0.8
4,289,985 + nrfE b4074 0.27 +0.4
4,289,985 + nrfE b4074 0.27 +0.4
4,290,140 + nrfE b4074 0.36 +0.2
4,290,255 + nrfE b4074 0.43 +0.2
4,290,255 + nrfE b4074 0.43 -1.4
4,290,265 - nrfE b4074 0.44 +0.2
4,290,415 - nrfE b4074 0.53 +0.6
4,290,415 - nrfE b4074 0.53 +0.1
4,290,441 - nrfE b4074 0.55 -0.2
4,290,443 + nrfE b4074 0.55 +0.5
4,290,443 + nrfE b4074 0.55 -0.2

Or see this region's nucleotide sequence