Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu6064 and Atu6065 are separated by 68 nucleotides Atu6065 and Atu6066 are separated by 139 nucleotides Atu6066 and Atu6067 are separated by 53 nucleotides Atu6067 and Atu6068 are separated by 156 nucleotides
Atu6064: Atu6064 - haloalkane dehalogenase, at 76,616 to 77,458
Atu6064
Atu6065: Atu6065 - conserved hypothetical protein, at 77,527 to 78,150
Atu6065
Atu6066: Atu6066 - acyl-CoA dehydrogenase, at 78,290 to 79,495
Atu6066
Atu6067: Atu6067 - conserved hypothetical protein, at 79,549 to 80,118
Atu6067
Atu6068: Atu6068 - ABC transporter, membrane spanning protein (ribose), at 80,275 to 81,309
Atu6068
Position (kb)
78
79
80 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 77.292 kb on + strand, within Atu6064 at 77.309 kb on - strand, within Atu6064 at 77.420 kb on + strand at 77.420 kb on + strand at 77.475 kb on - strand at 77.563 kb on + strand at 77.575 kb on + strand at 77.575 kb on + strand at 77.617 kb on - strand, within Atu6065 at 77.670 kb on + strand, within Atu6065 at 77.685 kb on - strand, within Atu6065 at 77.692 kb on - strand, within Atu6065 at 77.754 kb on - strand, within Atu6065 at 77.819 kb on - strand, within Atu6065 at 77.839 kb on + strand, within Atu6065 at 77.853 kb on + strand, within Atu6065 at 77.860 kb on + strand, within Atu6065 at 77.880 kb on - strand, within Atu6065 at 77.880 kb on - strand, within Atu6065 at 77.898 kb on + strand, within Atu6065 at 77.935 kb on + strand, within Atu6065 at 77.959 kb on - strand, within Atu6065 at 78.151 kb on + strand at 78.243 kb on + strand at 78.347 kb on + strand at 78.390 kb on - strand at 78.507 kb on - strand, within Atu6066 at 78.582 kb on + strand, within Atu6066 at 78.643 kb on + strand, within Atu6066 at 78.662 kb on - strand, within Atu6066 at 78.733 kb on + strand, within Atu6066 at 78.800 kb on + strand, within Atu6066 at 78.819 kb on + strand, within Atu6066 at 78.827 kb on - strand, within Atu6066 at 78.835 kb on + strand, within Atu6066 at 78.911 kb on + strand, within Atu6066 at 78.921 kb on - strand, within Atu6066 at 79.008 kb on + strand, within Atu6066 at 79.078 kb on + strand, within Atu6066 at 79.094 kb on + strand, within Atu6066 at 79.206 kb on + strand, within Atu6066 at 79.214 kb on - strand, within Atu6066 at 79.285 kb on + strand, within Atu6066 at 79.293 kb on - strand, within Atu6066 at 79.295 kb on - strand, within Atu6066 at 79.324 kb on - strand, within Atu6066 at 79.339 kb on + strand, within Atu6066 at 79.343 kb on - strand, within Atu6066 at 79.361 kb on - strand, within Atu6066 at 79.402 kb on + strand at 79.443 kb on - strand at 79.501 kb on + strand at 79.519 kb on + strand at 79.580 kb on + strand at 79.592 kb on - strand at 79.667 kb on + strand, within Atu6067 at 79.801 kb on + strand, within Atu6067 at 79.862 kb on - strand, within Atu6067 at 79.882 kb on - strand, within Atu6067 at 79.886 kb on - strand, within Atu6067 at 79.984 kb on - strand, within Atu6067 at 80.029 kb on + strand, within Atu6067 at 80.032 kb on - strand, within Atu6067 at 80.047 kb on - strand, within Atu6067 at 80.047 kb on - strand, within Atu6067 at 80.086 kb on - strand at 80.133 kb on + strand at 80.174 kb on - strand at 80.225 kb on + strand at 80.295 kb on - strand at 80.295 kb on - strand at 80.342 kb on + strand at 80.381 kb on - strand, within Atu6068 at 80.390 kb on - strand, within Atu6068 at 80.395 kb on + strand, within Atu6068 at 80.400 kb on + strand, within Atu6068 at 80.400 kb on + strand, within Atu6068 at 80.420 kb on - strand, within Atu6068 at 80.425 kb on - strand, within Atu6068 at 80.492 kb on + strand, within Atu6068
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 77,292 + Atu6064 0.80 +1.3 77,309 - Atu6064 0.82 +1.3 77,420 + +2.5 77,420 + +1.0 77,475 - +0.9 77,563 + +1.3 77,575 + +1.9 77,575 + +1.7 77,617 - Atu6065 0.14 -0.6 77,670 + Atu6065 0.23 -0.5 77,685 - Atu6065 0.25 +0.5 77,692 - Atu6065 0.26 +1.3 77,754 - Atu6065 0.36 +0.6 77,819 - Atu6065 0.47 +0.9 77,839 + Atu6065 0.50 +1.9 77,853 + Atu6065 0.52 +0.2 77,860 + Atu6065 0.53 +0.0 77,880 - Atu6065 0.57 -0.3 77,880 - Atu6065 0.57 +1.3 77,898 + Atu6065 0.59 +2.3 77,935 + Atu6065 0.65 +1.3 77,959 - Atu6065 0.69 -0.4 78,151 + -1.3 78,243 + +0.7 78,347 + +0.6 78,390 - +0.3 78,507 - Atu6066 0.18 -1.2 78,582 + Atu6066 0.24 +0.6 78,643 + Atu6066 0.29 +1.3 78,662 - Atu6066 0.31 +0.0 78,733 + Atu6066 0.37 +1.3 78,800 + Atu6066 0.42 -0.4 78,819 + Atu6066 0.44 -0.5 78,827 - Atu6066 0.45 +0.6 78,835 + Atu6066 0.45 -1.1 78,911 + Atu6066 0.51 +1.3 78,921 - Atu6066 0.52 +0.2 79,008 + Atu6066 0.60 +0.9 79,078 + Atu6066 0.65 +3.8 79,094 + Atu6066 0.67 +0.4 79,206 + Atu6066 0.76 +0.6 79,214 - Atu6066 0.77 -0.4 79,285 + Atu6066 0.83 -0.5 79,293 - Atu6066 0.83 -2.5 79,295 - Atu6066 0.83 -0.5 79,324 - Atu6066 0.86 -0.9 79,339 + Atu6066 0.87 -0.6 79,343 - Atu6066 0.87 -1.1 79,361 - Atu6066 0.89 -1.1 79,402 + +0.0 79,443 - +2.0 79,501 + -1.3 79,519 + +0.3 79,580 + +1.4 79,592 - -0.3 79,667 + Atu6067 0.21 -0.1 79,801 + Atu6067 0.44 -0.2 79,862 - Atu6067 0.55 +0.3 79,882 - Atu6067 0.58 +2.3 79,886 - Atu6067 0.59 +1.1 79,984 - Atu6067 0.76 -0.0 80,029 + Atu6067 0.84 +0.0 80,032 - Atu6067 0.85 +0.9 80,047 - Atu6067 0.87 +0.1 80,047 - Atu6067 0.87 -0.2 80,086 - +0.3 80,133 + -1.9 80,174 - -0.1 80,225 + -0.1 80,295 - +0.5 80,295 - +4.1 80,342 + -0.2 80,381 - Atu6068 0.10 +0.9 80,390 - Atu6068 0.11 +0.4 80,395 + Atu6068 0.12 +2.8 80,400 + Atu6068 0.12 -0.5 80,400 + Atu6068 0.12 -0.2 80,420 - Atu6068 0.14 +0.3 80,425 - Atu6068 0.14 +1.3 80,492 + Atu6068 0.21 -0.3
Or see this region's nucleotide sequence