Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5138 and PP_5139 are separated by 9 nucleotides PP_5139 and PP_5140 are separated by 83 nucleotides PP_5140 and PP_5141 are separated by 56 nucleotides
PP_5138: PP_5138 - putative transcriptional regulator LysR family, at 5,863,019 to 5,863,888
_5138
PP_5139: PP_5139 - cadmium translocating P-type ATPase, at 5,863,898 to 5,866,150
_5139
PP_5140: PP_5140 - Transcriptional regulator, MerR family, at 5,866,234 to 5,866,677
_5140
PP_5141: PP_5141 - Thymidylate synthase, at 5,866,734 to 5,867,705
_5141
Position (kb) 5863
5864
5865
5866
5867 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5862.901 kb on - strand at 5862.931 kb on - strand at 5862.931 kb on - strand at 5862.970 kb on + strand at 5862.970 kb on + strand at 5862.970 kb on + strand at 5862.970 kb on + strand at 5862.971 kb on - strand at 5862.971 kb on - strand at 5862.971 kb on - strand at 5863.020 kb on + strand at 5863.101 kb on + strand at 5863.101 kb on + strand at 5863.101 kb on + strand at 5863.101 kb on + strand at 5863.107 kb on + strand, within PP_5138 at 5863.107 kb on + strand, within PP_5138 at 5863.108 kb on - strand, within PP_5138 at 5863.108 kb on - strand, within PP_5138 at 5863.108 kb on - strand, within PP_5138 at 5863.108 kb on - strand, within PP_5138 at 5863.424 kb on + strand, within PP_5138 at 5863.564 kb on - strand, within PP_5138 at 5863.564 kb on - strand, within PP_5138 at 5863.587 kb on + strand, within PP_5138 at 5863.587 kb on + strand at 5863.587 kb on + strand, within PP_5138 at 5863.587 kb on + strand, within PP_5138 at 5863.587 kb on + strand, within PP_5138 at 5863.587 kb on + strand, within PP_5138 at 5863.588 kb on - strand, within PP_5138 at 5863.588 kb on - strand, within PP_5138 at 5863.588 kb on - strand, within PP_5138 at 5863.588 kb on - strand, within PP_5138 at 5863.588 kb on - strand, within PP_5138 at 5863.588 kb on - strand, within PP_5138 at 5863.590 kb on + strand, within PP_5138 at 5863.590 kb on + strand, within PP_5138 at 5863.590 kb on + strand, within PP_5138 at 5863.590 kb on + strand, within PP_5138 at 5863.704 kb on - strand, within PP_5138 at 5863.744 kb on - strand, within PP_5138 at 5863.860 kb on + strand at 5863.860 kb on + strand at 5863.860 kb on + strand at 5863.861 kb on - strand at 5863.861 kb on - strand at 5863.861 kb on - strand at 5863.861 kb on - strand at 5863.861 kb on - strand at 5863.861 kb on - strand at 5863.861 kb on - strand at 5863.861 kb on - strand at 5863.890 kb on + strand at 5863.890 kb on + strand at 5863.890 kb on + strand at 5863.890 kb on + strand at 5863.890 kb on + strand at 5863.890 kb on + strand at 5863.890 kb on + strand at 5863.890 kb on + strand at 5863.890 kb on + strand at 5863.891 kb on - strand at 5863.891 kb on - strand at 5863.891 kb on - strand at 5863.891 kb on - strand at 5863.891 kb on - strand at 5863.899 kb on + strand at 5863.899 kb on + strand at 5863.900 kb on - strand at 5864.247 kb on + strand, within PP_5139 at 5864.247 kb on + strand, within PP_5139 at 5864.574 kb on + strand, within PP_5139 at 5865.014 kb on + strand, within PP_5139 at 5865.057 kb on + strand, within PP_5139 at 5865.057 kb on + strand, within PP_5139 at 5865.058 kb on - strand, within PP_5139 at 5865.091 kb on + strand, within PP_5139 at 5865.508 kb on + strand, within PP_5139 at 5865.537 kb on - strand, within PP_5139 at 5865.556 kb on + strand, within PP_5139 at 5865.556 kb on + strand, within PP_5139 at 5865.556 kb on + strand, within PP_5139 at 5865.557 kb on - strand, within PP_5139 at 5865.615 kb on + strand, within PP_5139 at 5865.615 kb on + strand, within PP_5139 at 5865.616 kb on - strand at 5865.616 kb on - strand, within PP_5139 at 5866.203 kb on + strand at 5866.288 kb on + strand, within PP_5140 at 5866.288 kb on + strand, within PP_5140 at 5866.291 kb on + strand, within PP_5140 at 5866.292 kb on - strand, within PP_5140 at 5866.292 kb on - strand, within PP_5140 at 5866.339 kb on + strand at 5866.340 kb on - strand, within PP_5140 at 5866.349 kb on - strand, within PP_5140 at 5866.724 kb on + strand at 5866.725 kb on - strand at 5866.725 kb on - strand at 5866.732 kb on + strand at 5866.732 kb on + strand at 5866.732 kb on + strand at 5866.732 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,862,901 - -0.3 5,862,931 - +0.3 5,862,931 - -1.8 5,862,970 + -0.5 5,862,970 + +0.8 5,862,970 + +0.4 5,862,970 + -0.0 5,862,971 - -0.4 5,862,971 - +0.2 5,862,971 - +0.7 5,863,020 + -0.8 5,863,101 + -0.5 5,863,101 + +0.6 5,863,101 + -0.6 5,863,101 + +0.3 5,863,107 + PP_5138 0.10 +1.3 5,863,107 + PP_5138 0.10 +1.1 5,863,108 - PP_5138 0.10 -0.1 5,863,108 - PP_5138 0.10 -0.7 5,863,108 - PP_5138 0.10 +0.4 5,863,108 - PP_5138 0.10 +0.7 5,863,424 + PP_5138 0.47 -0.0 5,863,564 - PP_5138 0.63 -0.6 5,863,564 - PP_5138 0.63 +0.1 5,863,587 + PP_5138 0.65 -0.4 5,863,587 + +1.9 5,863,587 + PP_5138 0.65 -0.0 5,863,587 + PP_5138 0.65 -0.0 5,863,587 + PP_5138 0.65 +1.6 5,863,587 + PP_5138 0.65 -0.8 5,863,588 - PP_5138 0.65 -0.7 5,863,588 - PP_5138 0.65 +0.6 5,863,588 - PP_5138 0.65 -1.1 5,863,588 - PP_5138 0.65 -0.2 5,863,588 - PP_5138 0.65 +0.3 5,863,588 - PP_5138 0.65 -1.5 5,863,590 + PP_5138 0.66 -1.0 5,863,590 + PP_5138 0.66 +0.6 5,863,590 + PP_5138 0.66 -0.3 5,863,590 + PP_5138 0.66 -0.2 5,863,704 - PP_5138 0.79 -0.6 5,863,744 - PP_5138 0.83 -1.3 5,863,860 + +1.3 5,863,860 + -0.6 5,863,860 + -1.3 5,863,861 - -0.5 5,863,861 - +2.2 5,863,861 - -0.7 5,863,861 - +0.1 5,863,861 - +0.4 5,863,861 - -0.9 5,863,861 - -1.5 5,863,861 - -0.1 5,863,890 + -0.3 5,863,890 + +0.3 5,863,890 + +0.3 5,863,890 + -1.1 5,863,890 + -0.3 5,863,890 + +0.8 5,863,890 + -1.6 5,863,890 + -0.4 5,863,890 + +0.0 5,863,891 - -0.3 5,863,891 - +0.8 5,863,891 - -0.6 5,863,891 - -2.5 5,863,891 - +0.2 5,863,899 + +0.4 5,863,899 + +0.9 5,863,900 - +0.2 5,864,247 + PP_5139 0.15 -0.5 5,864,247 + PP_5139 0.15 +0.4 5,864,574 + PP_5139 0.30 +0.2 5,865,014 + PP_5139 0.50 +0.6 5,865,057 + PP_5139 0.51 -0.8 5,865,057 + PP_5139 0.51 -1.5 5,865,058 - PP_5139 0.51 -3.1 5,865,091 + PP_5139 0.53 -0.3 5,865,508 + PP_5139 0.71 +0.1 5,865,537 - PP_5139 0.73 +0.0 5,865,556 + PP_5139 0.74 -1.4 5,865,556 + PP_5139 0.74 -0.5 5,865,556 + PP_5139 0.74 -0.6 5,865,557 - PP_5139 0.74 +0.7 5,865,615 + PP_5139 0.76 -0.6 5,865,615 + PP_5139 0.76 +2.9 5,865,616 - +2.9 5,865,616 - PP_5139 0.76 +0.9 5,866,203 + -1.7 5,866,288 + PP_5140 0.12 +0.3 5,866,288 + PP_5140 0.12 -1.2 5,866,291 + PP_5140 0.13 -0.2 5,866,292 - PP_5140 0.13 +1.9 5,866,292 - PP_5140 0.13 +0.7 5,866,339 + +2.1 5,866,340 - PP_5140 0.24 +2.0 5,866,349 - PP_5140 0.26 -0.2 5,866,724 + +0.5 5,866,725 - +1.1 5,866,725 - +0.8 5,866,732 + +0.5 5,866,732 + -0.7 5,866,732 + -1.2 5,866,732 + +0.1
Or see this region's nucleotide sequence