Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5030 and PP_5031 are separated by 15 nucleotides PP_5031 and PP_5032 are separated by 156 nucleotides
PP_5030: PP_5030 - Imidazolonepropionase, at 5,731,350 to 5,732,555
_5030
PP_5031: PP_5031 - proline (histidine) APC transporter, at 5,732,571 to 5,733,974
_5031
PP_5032: PP_5032 - Histidine ammonia-lyase, at 5,734,131 to 5,735,663
_5032
Position (kb) 5732
5733
5734 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5731.700 kb on - strand, within PP_5030 at 5732.005 kb on + strand, within PP_5030 at 5732.006 kb on - strand, within PP_5030 at 5732.184 kb on + strand, within PP_5030 at 5732.185 kb on - strand, within PP_5030 at 5732.185 kb on - strand, within PP_5030 at 5732.243 kb on + strand, within PP_5030 at 5732.293 kb on + strand, within PP_5030 at 5732.293 kb on + strand, within PP_5030 at 5732.293 kb on + strand, within PP_5030 at 5732.294 kb on - strand, within PP_5030 at 5732.294 kb on - strand, within PP_5030 at 5732.572 kb on + strand at 5732.642 kb on - strand at 5732.659 kb on - strand at 5732.659 kb on - strand at 5732.659 kb on - strand at 5732.707 kb on + strand at 5732.830 kb on - strand, within PP_5031 at 5732.920 kb on + strand, within PP_5031 at 5732.921 kb on - strand, within PP_5031 at 5732.921 kb on - strand, within PP_5031 at 5732.932 kb on - strand, within PP_5031 at 5732.980 kb on - strand, within PP_5031 at 5733.040 kb on + strand, within PP_5031 at 5733.040 kb on + strand, within PP_5031 at 5733.040 kb on + strand, within PP_5031 at 5733.040 kb on + strand, within PP_5031 at 5733.041 kb on - strand, within PP_5031 at 5733.041 kb on - strand, within PP_5031 at 5733.041 kb on - strand, within PP_5031 at 5733.196 kb on + strand, within PP_5031 at 5733.196 kb on + strand, within PP_5031 at 5733.229 kb on + strand, within PP_5031 at 5733.230 kb on - strand, within PP_5031 at 5733.543 kb on + strand, within PP_5031 at 5733.558 kb on + strand, within PP_5031 at 5733.558 kb on + strand, within PP_5031 at 5733.559 kb on - strand, within PP_5031 at 5733.559 kb on - strand, within PP_5031 at 5733.637 kb on + strand, within PP_5031 at 5733.637 kb on + strand, within PP_5031 at 5733.637 kb on + strand, within PP_5031 at 5733.638 kb on - strand, within PP_5031 at 5733.638 kb on - strand, within PP_5031 at 5733.638 kb on - strand, within PP_5031 at 5733.638 kb on - strand, within PP_5031 at 5733.638 kb on - strand, within PP_5031 at 5733.641 kb on + strand, within PP_5031 at 5733.642 kb on - strand, within PP_5031 at 5733.691 kb on + strand, within PP_5031 at 5733.691 kb on + strand, within PP_5031 at 5733.691 kb on + strand, within PP_5031 at 5733.730 kb on + strand, within PP_5031 at 5733.731 kb on - strand, within PP_5031 at 5733.742 kb on + strand, within PP_5031 at 5733.742 kb on + strand, within PP_5031 at 5733.832 kb on + strand, within PP_5031 at 5733.883 kb on + strand at 5734.076 kb on + strand at 5734.076 kb on + strand at 5734.077 kb on - strand at 5734.077 kb on - strand at 5734.175 kb on + strand at 5734.238 kb on - strand at 5734.238 kb on - strand at 5734.755 kb on + strand, within PP_5032 at 5734.755 kb on + strand, within PP_5032
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,731,700 - PP_5030 0.29 +0.5 5,732,005 + PP_5030 0.54 +0.2 5,732,006 - PP_5030 0.54 +1.2 5,732,184 + PP_5030 0.69 +0.1 5,732,185 - PP_5030 0.69 -1.1 5,732,185 - PP_5030 0.69 +1.4 5,732,243 + PP_5030 0.74 -0.1 5,732,293 + PP_5030 0.78 +1.8 5,732,293 + PP_5030 0.78 -0.3 5,732,293 + PP_5030 0.78 +0.6 5,732,294 - PP_5030 0.78 +0.0 5,732,294 - PP_5030 0.78 +1.2 5,732,572 + -0.7 5,732,642 - -0.4 5,732,659 - +1.1 5,732,659 - +0.1 5,732,659 - +1.9 5,732,707 + -1.3 5,732,830 - PP_5031 0.18 -0.9 5,732,920 + PP_5031 0.25 +0.3 5,732,921 - PP_5031 0.25 -1.1 5,732,921 - PP_5031 0.25 -0.1 5,732,932 - PP_5031 0.26 +0.5 5,732,980 - PP_5031 0.29 +2.9 5,733,040 + PP_5031 0.33 -0.2 5,733,040 + PP_5031 0.33 -1.7 5,733,040 + PP_5031 0.33 +0.1 5,733,040 + PP_5031 0.33 -0.6 5,733,041 - PP_5031 0.33 +0.3 5,733,041 - PP_5031 0.33 +0.2 5,733,041 - PP_5031 0.33 +1.2 5,733,196 + PP_5031 0.45 +3.1 5,733,196 + PP_5031 0.45 +0.8 5,733,229 + PP_5031 0.47 -0.4 5,733,230 - PP_5031 0.47 -0.2 5,733,543 + PP_5031 0.69 +0.1 5,733,558 + PP_5031 0.70 +0.6 5,733,558 + PP_5031 0.70 +0.6 5,733,559 - PP_5031 0.70 -1.4 5,733,559 - PP_5031 0.70 +0.9 5,733,637 + PP_5031 0.76 +0.3 5,733,637 + PP_5031 0.76 -0.5 5,733,637 + PP_5031 0.76 -0.7 5,733,638 - PP_5031 0.76 +1.7 5,733,638 - PP_5031 0.76 +0.7 5,733,638 - PP_5031 0.76 +3.2 5,733,638 - PP_5031 0.76 -2.0 5,733,638 - PP_5031 0.76 -0.4 5,733,641 + PP_5031 0.76 +0.4 5,733,642 - PP_5031 0.76 +0.5 5,733,691 + PP_5031 0.80 +0.4 5,733,691 + PP_5031 0.80 -0.1 5,733,691 + PP_5031 0.80 +1.5 5,733,730 + PP_5031 0.83 +0.6 5,733,731 - PP_5031 0.83 -0.5 5,733,742 + PP_5031 0.83 +0.5 5,733,742 + PP_5031 0.83 +0.1 5,733,832 + PP_5031 0.90 -1.3 5,733,883 + -1.0 5,734,076 + -1.1 5,734,076 + -1.0 5,734,077 - -0.5 5,734,077 - +1.8 5,734,175 + -0.4 5,734,238 - +0.6 5,734,238 - +0.4 5,734,755 + PP_5032 0.41 -1.0 5,734,755 + PP_5032 0.41 +1.3
Or see this region's nucleotide sequence