Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4831 and PP_4832 overlap by 4 nucleotides PP_4832 and PP_4833 are separated by 134 nucleotides PP_4833 and PP_4834 are separated by 26 nucleotides
PP_4831: PP_4831 - putative cobalt-precorrin-6A synthase, at 5,498,132 to 5,499,226
_4831
PP_4832: PP_4832 - Precorrin-6x reductase, at 5,499,223 to 5,499,942
_4832
PP_4833: PP_4833 - SURF1 domain protein, at 5,500,077 to 5,500,508
_4833
PP_4834: PP_4834 - SPFH domain/Band 7 family protein, at 5,500,535 to 5,501,389
_4834
Position (kb) 5500
5501 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5499.302 kb on - strand, within PP_4832 at 5499.302 kb on - strand, within PP_4832 at 5499.302 kb on - strand, within PP_4832 at 5499.302 kb on - strand, within PP_4832 at 5499.391 kb on + strand, within PP_4832 at 5499.439 kb on + strand, within PP_4832 at 5499.440 kb on - strand, within PP_4832 at 5499.440 kb on - strand, within PP_4832 at 5499.440 kb on - strand, within PP_4832 at 5499.940 kb on + strand at 5499.974 kb on + strand at 5499.974 kb on + strand at 5499.974 kb on + strand at 5500.035 kb on - strand at 5500.071 kb on - strand at 5500.151 kb on - strand, within PP_4833 at 5500.151 kb on - strand, within PP_4833 at 5500.187 kb on + strand, within PP_4833 at 5500.187 kb on + strand, within PP_4833 at 5500.187 kb on + strand, within PP_4833 at 5500.241 kb on - strand, within PP_4833 at 5500.517 kb on + strand at 5500.518 kb on - strand at 5500.691 kb on + strand, within PP_4834 at 5500.691 kb on + strand, within PP_4834 at 5500.691 kb on + strand, within PP_4834 at 5500.812 kb on + strand, within PP_4834 at 5500.813 kb on - strand, within PP_4834 at 5500.813 kb on - strand, within PP_4834 at 5500.813 kb on - strand, within PP_4834 at 5500.835 kb on + strand, within PP_4834 at 5500.835 kb on + strand, within PP_4834 at 5500.835 kb on + strand, within PP_4834 at 5500.835 kb on + strand, within PP_4834 at 5500.835 kb on + strand, within PP_4834 at 5500.835 kb on + strand, within PP_4834 at 5500.835 kb on + strand, within PP_4834 at 5500.836 kb on - strand, within PP_4834 at 5500.836 kb on - strand, within PP_4834 at 5500.836 kb on - strand, within PP_4834 at 5500.836 kb on - strand, within PP_4834 at 5501.261 kb on + strand, within PP_4834 at 5501.261 kb on + strand, within PP_4834 at 5501.261 kb on + strand, within PP_4834 at 5501.261 kb on + strand, within PP_4834 at 5501.262 kb on - strand, within PP_4834 at 5501.262 kb on - strand, within PP_4834 at 5501.262 kb on - strand, within PP_4834 at 5501.262 kb on - strand, within PP_4834 at 5501.265 kb on + strand, within PP_4834 at 5501.265 kb on + strand, within PP_4834 at 5501.279 kb on + strand, within PP_4834 at 5501.279 kb on + strand, within PP_4834 at 5501.280 kb on - strand, within PP_4834 at 5501.280 kb on - strand, within PP_4834 at 5501.280 kb on - strand, within PP_4834 at 5501.280 kb on - strand, within PP_4834 at 5501.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,499,302 - PP_4832 0.11 +0.8 5,499,302 - PP_4832 0.11 +1.6 5,499,302 - PP_4832 0.11 -0.2 5,499,302 - PP_4832 0.11 +1.0 5,499,391 + PP_4832 0.23 -0.3 5,499,439 + PP_4832 0.30 +0.5 5,499,440 - PP_4832 0.30 -0.7 5,499,440 - PP_4832 0.30 -0.6 5,499,440 - PP_4832 0.30 +0.9 5,499,940 + -0.2 5,499,974 + -0.1 5,499,974 + -0.1 5,499,974 + +1.6 5,500,035 - +0.3 5,500,071 - -0.0 5,500,151 - PP_4833 0.17 -0.5 5,500,151 - PP_4833 0.17 +0.2 5,500,187 + PP_4833 0.25 -0.2 5,500,187 + PP_4833 0.25 -1.3 5,500,187 + PP_4833 0.25 -1.2 5,500,241 - PP_4833 0.38 -1.2 5,500,517 + +3.4 5,500,518 - -0.3 5,500,691 + PP_4834 0.18 +0.7 5,500,691 + PP_4834 0.18 +0.2 5,500,691 + PP_4834 0.18 -0.2 5,500,812 + PP_4834 0.32 -0.7 5,500,813 - PP_4834 0.33 +0.3 5,500,813 - PP_4834 0.33 -2.0 5,500,813 - PP_4834 0.33 -1.2 5,500,835 + PP_4834 0.35 +0.9 5,500,835 + PP_4834 0.35 +1.4 5,500,835 + PP_4834 0.35 +0.5 5,500,835 + PP_4834 0.35 -0.2 5,500,835 + PP_4834 0.35 -0.3 5,500,835 + PP_4834 0.35 +0.3 5,500,835 + PP_4834 0.35 +0.0 5,500,836 - PP_4834 0.35 -0.4 5,500,836 - PP_4834 0.35 -0.2 5,500,836 - PP_4834 0.35 +0.7 5,500,836 - PP_4834 0.35 +2.9 5,501,261 + PP_4834 0.85 +0.9 5,501,261 + PP_4834 0.85 +0.1 5,501,261 + PP_4834 0.85 +0.1 5,501,261 + PP_4834 0.85 -0.8 5,501,262 - PP_4834 0.85 +0.5 5,501,262 - PP_4834 0.85 -0.4 5,501,262 - PP_4834 0.85 -0.9 5,501,262 - PP_4834 0.85 +0.5 5,501,265 + PP_4834 0.85 +1.8 5,501,265 + PP_4834 0.85 -0.2 5,501,279 + PP_4834 0.87 +0.9 5,501,279 + PP_4834 0.87 -0.1 5,501,280 - PP_4834 0.87 -0.4 5,501,280 - PP_4834 0.87 -0.8 5,501,280 - PP_4834 0.87 +0.2 5,501,280 - PP_4834 0.87 -0.1 5,501,448 + -0.2
Or see this region's nucleotide sequence