Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4623 and PP_4624 are separated by 255 nucleotides PP_4624 and PP_4625 are separated by 176 nucleotides PP_4625 and PP_4626 overlap by 11 nucleotides
PP_4623: PP_4623 - conserved protein of unknown function, at 5,245,358 to 5,248,660
_4623
PP_4624: PP_4624 - putative hydrolase, alpha/beta fold family, at 5,248,916 to 5,249,821
_4624
PP_4625: PP_4625 - anti-holin regulator of holin CidA, at 5,249,998 to 5,250,684
_4625
PP_4626: PP_4626 - holin regulator of murein hydrolases, at 5,250,674 to 5,251,060
_4626
Position (kb) 5248
5249
5250 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5248.071 kb on + strand, within PP_4623 at 5248.071 kb on + strand, within PP_4623 at 5248.071 kb on + strand, within PP_4623 at 5248.071 kb on + strand, within PP_4623 at 5248.072 kb on - strand, within PP_4623 at 5248.072 kb on - strand, within PP_4623 at 5248.170 kb on + strand, within PP_4623 at 5248.170 kb on + strand, within PP_4623 at 5248.170 kb on + strand, within PP_4623 at 5248.171 kb on - strand, within PP_4623 at 5248.171 kb on - strand, within PP_4623 at 5248.171 kb on - strand, within PP_4623 at 5248.171 kb on - strand, within PP_4623 at 5248.171 kb on - strand, within PP_4623 at 5248.240 kb on + strand, within PP_4623 at 5248.240 kb on + strand, within PP_4623 at 5248.297 kb on + strand, within PP_4623 at 5248.313 kb on + strand, within PP_4623 at 5248.459 kb on + strand at 5248.652 kb on - strand at 5248.678 kb on + strand at 5248.678 kb on + strand at 5248.679 kb on - strand at 5248.679 kb on - strand at 5248.705 kb on + strand at 5248.731 kb on - strand at 5248.801 kb on - strand at 5248.941 kb on + strand at 5248.974 kb on + strand at 5248.975 kb on - strand at 5248.975 kb on - strand at 5249.005 kb on + strand at 5249.005 kb on + strand at 5249.030 kb on + strand, within PP_4624 at 5249.031 kb on - strand, within PP_4624 at 5249.137 kb on + strand, within PP_4624 at 5249.137 kb on + strand, within PP_4624 at 5249.137 kb on + strand, within PP_4624 at 5249.137 kb on + strand, within PP_4624 at 5249.138 kb on - strand, within PP_4624 at 5249.190 kb on + strand, within PP_4624 at 5249.289 kb on + strand, within PP_4624 at 5249.289 kb on + strand, within PP_4624 at 5249.289 kb on + strand, within PP_4624 at 5249.289 kb on + strand, within PP_4624 at 5249.290 kb on - strand, within PP_4624 at 5249.305 kb on + strand, within PP_4624 at 5249.305 kb on + strand, within PP_4624 at 5249.305 kb on + strand, within PP_4624 at 5249.305 kb on + strand, within PP_4624 at 5249.305 kb on + strand, within PP_4624 at 5249.381 kb on + strand, within PP_4624 at 5249.381 kb on + strand, within PP_4624 at 5249.381 kb on + strand, within PP_4624 at 5249.382 kb on - strand, within PP_4624 at 5249.382 kb on - strand, within PP_4624 at 5249.382 kb on - strand, within PP_4624 at 5249.382 kb on - strand, within PP_4624 at 5249.580 kb on + strand, within PP_4624 at 5249.580 kb on + strand, within PP_4624 at 5249.581 kb on - strand, within PP_4624 at 5249.581 kb on - strand, within PP_4624 at 5249.671 kb on - strand, within PP_4624 at 5249.671 kb on - strand, within PP_4624 at 5249.705 kb on + strand, within PP_4624 at 5249.705 kb on + strand, within PP_4624 at 5249.705 kb on + strand, within PP_4624 at 5249.743 kb on - strand at 5249.743 kb on - strand at 5249.838 kb on + strand at 5249.838 kb on + strand at 5249.838 kb on + strand at 5249.838 kb on + strand at 5249.838 kb on + strand at 5249.839 kb on - strand at 5249.845 kb on - strand at 5249.855 kb on + strand at 5249.856 kb on - strand at 5249.856 kb on - strand at 5249.856 kb on - strand at 5249.894 kb on + strand at 5249.894 kb on + strand at 5249.894 kb on + strand at 5249.894 kb on + strand at 5249.894 kb on + strand at 5249.894 kb on + strand at 5249.894 kb on + strand at 5249.894 kb on + strand at 5249.894 kb on + strand at 5249.895 kb on - strand at 5249.895 kb on - strand at 5249.895 kb on - strand at 5249.895 kb on - strand at 5249.895 kb on - strand at 5249.895 kb on - strand at 5249.895 kb on - strand at 5249.895 kb on - strand at 5249.907 kb on + strand at 5249.907 kb on + strand at 5249.908 kb on - strand at 5249.908 kb on - strand at 5249.908 kb on - strand at 5249.908 kb on - strand at 5249.908 kb on - strand at 5250.037 kb on - strand at 5250.162 kb on + strand, within PP_4625 at 5250.524 kb on + strand, within PP_4625 at 5250.594 kb on - strand, within PP_4625 at 5250.611 kb on + strand, within PP_4625 at 5250.612 kb on - strand, within PP_4625 at 5250.612 kb on - strand, within PP_4625 at 5250.632 kb on + strand at 5250.731 kb on + strand, within PP_4626 at 5250.731 kb on + strand, within PP_4626 at 5250.731 kb on + strand, within PP_4626 at 5250.732 kb on - strand, within PP_4626 at 5250.741 kb on - strand, within PP_4626 at 5250.741 kb on - strand, within PP_4626 at 5250.804 kb on + strand, within PP_4626
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,248,071 + PP_4623 0.82 +0.4 5,248,071 + PP_4623 0.82 +1.6 5,248,071 + PP_4623 0.82 -0.7 5,248,071 + PP_4623 0.82 +2.7 5,248,072 - PP_4623 0.82 +0.3 5,248,072 - PP_4623 0.82 -1.0 5,248,170 + PP_4623 0.85 +0.2 5,248,170 + PP_4623 0.85 -0.8 5,248,170 + PP_4623 0.85 +0.8 5,248,171 - PP_4623 0.85 -1.5 5,248,171 - PP_4623 0.85 -0.1 5,248,171 - PP_4623 0.85 +0.1 5,248,171 - PP_4623 0.85 -0.4 5,248,171 - PP_4623 0.85 +0.2 5,248,240 + PP_4623 0.87 -0.2 5,248,240 + PP_4623 0.87 -0.6 5,248,297 + PP_4623 0.89 +0.5 5,248,313 + PP_4623 0.89 +2.9 5,248,459 + +0.7 5,248,652 - -0.1 5,248,678 + +0.1 5,248,678 + -0.7 5,248,679 - +0.6 5,248,679 - -0.2 5,248,705 + +0.5 5,248,731 - +0.0 5,248,801 - -0.7 5,248,941 + -0.9 5,248,974 + -0.4 5,248,975 - -0.1 5,248,975 - +0.0 5,249,005 + +0.3 5,249,005 + -0.9 5,249,030 + PP_4624 0.13 -1.5 5,249,031 - PP_4624 0.13 -1.5 5,249,137 + PP_4624 0.24 -0.5 5,249,137 + PP_4624 0.24 +2.9 5,249,137 + PP_4624 0.24 -1.7 5,249,137 + PP_4624 0.24 +0.7 5,249,138 - PP_4624 0.25 -0.6 5,249,190 + PP_4624 0.30 -0.7 5,249,289 + PP_4624 0.41 +0.1 5,249,289 + PP_4624 0.41 +0.1 5,249,289 + PP_4624 0.41 -1.6 5,249,289 + PP_4624 0.41 -2.4 5,249,290 - PP_4624 0.41 -0.8 5,249,305 + PP_4624 0.43 +1.5 5,249,305 + PP_4624 0.43 -2.2 5,249,305 + PP_4624 0.43 -0.6 5,249,305 + PP_4624 0.43 +0.1 5,249,305 + PP_4624 0.43 +0.1 5,249,381 + PP_4624 0.51 -0.5 5,249,381 + PP_4624 0.51 -0.7 5,249,381 + PP_4624 0.51 +0.6 5,249,382 - PP_4624 0.51 -2.1 5,249,382 - PP_4624 0.51 -0.1 5,249,382 - PP_4624 0.51 -0.3 5,249,382 - PP_4624 0.51 -1.4 5,249,580 + PP_4624 0.73 -0.1 5,249,580 + PP_4624 0.73 -0.3 5,249,581 - PP_4624 0.73 -1.2 5,249,581 - PP_4624 0.73 -1.1 5,249,671 - PP_4624 0.83 -0.1 5,249,671 - PP_4624 0.83 +0.3 5,249,705 + PP_4624 0.87 -1.2 5,249,705 + PP_4624 0.87 +0.3 5,249,705 + PP_4624 0.87 -1.5 5,249,743 - +1.0 5,249,743 - -0.8 5,249,838 + -1.7 5,249,838 + +2.9 5,249,838 + +0.7 5,249,838 + -1.5 5,249,838 + +0.4 5,249,839 - +0.2 5,249,845 - -1.0 5,249,855 + -1.6 5,249,856 - -0.9 5,249,856 - -1.5 5,249,856 - +3.9 5,249,894 + -1.7 5,249,894 + -1.8 5,249,894 + -0.8 5,249,894 + -0.6 5,249,894 + -0.2 5,249,894 + -0.2 5,249,894 + +0.3 5,249,894 + +0.7 5,249,894 + -0.5 5,249,895 - +0.5 5,249,895 - +0.1 5,249,895 - -1.7 5,249,895 - -0.5 5,249,895 - -0.2 5,249,895 - +0.7 5,249,895 - +0.4 5,249,895 - -0.7 5,249,907 + -2.3 5,249,907 + -1.1 5,249,908 - +0.5 5,249,908 - +0.7 5,249,908 - +0.2 5,249,908 - -0.3 5,249,908 - +0.1 5,250,037 - -0.0 5,250,162 + PP_4625 0.24 +0.6 5,250,524 + PP_4625 0.77 -0.9 5,250,594 - PP_4625 0.87 -1.2 5,250,611 + PP_4625 0.89 -1.0 5,250,612 - PP_4625 0.89 -0.6 5,250,612 - PP_4625 0.89 -1.9 5,250,632 + +1.2 5,250,731 + PP_4626 0.15 -0.4 5,250,731 + PP_4626 0.15 +1.1 5,250,731 + PP_4626 0.15 -2.8 5,250,732 - PP_4626 0.15 -0.9 5,250,741 - PP_4626 0.17 +0.6 5,250,741 - PP_4626 0.17 +1.0 5,250,804 + PP_4626 0.34 +1.3
Or see this region's nucleotide sequence