Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4468 and PP_t58 are separated by 319 nucleotides PP_t58 and PP_t57 are separated by 134 nucleotides PP_t57 and PP_4469 are separated by 110 nucleotides PP_4469 and PP_5693 overlap by 1 nucleotides PP_5693 and PP_4470 are separated by 69 nucleotides PP_4470 and PP_4471 are separated by 107 nucleotides
PP_4468: PP_4468 - Transcriptional regulator, Cro/CI family, at 5,074,745 to 5,075,236
_4468
PP_t58: PP_t58 - tRNA-Arg, at 5,075,556 to 5,075,627
_t58
PP_t57: PP_t57 - tRNA-Arg, at 5,075,762 to 5,075,830
_t57
PP_4469: PP_4469 - ribose 1,5-bisphosphate phosphokinase, at 5,075,941 to 5,076,540
_4469
PP_5693: PP_5693 - conserved protein of unknown function, at 5,076,540 to 5,076,809
_5693
PP_4470: PP_4470 - putative transcriptional regulator (Arc domain-containing), at 5,076,879 to 5,077,205
_4470
PP_4471: PP_4471 - Magnesium transporter, at 5,077,313 to 5,078,755
_4471
Position (kb) 5075
5076
5077 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5074.983 kb on + strand, within PP_4468 at 5074.983 kb on + strand, within PP_4468 at 5075.037 kb on + strand, within PP_4468 at 5075.037 kb on + strand, within PP_4468 at 5075.038 kb on - strand, within PP_4468 at 5075.067 kb on + strand, within PP_4468 at 5075.067 kb on + strand at 5075.067 kb on + strand, within PP_4468 at 5075.067 kb on + strand, within PP_4468 at 5075.067 kb on + strand, within PP_4468 at 5075.068 kb on - strand, within PP_4468 at 5075.068 kb on - strand, within PP_4468 at 5075.099 kb on - strand, within PP_4468 at 5075.099 kb on - strand, within PP_4468 at 5075.099 kb on - strand, within PP_4468 at 5075.109 kb on + strand, within PP_4468 at 5075.109 kb on + strand, within PP_4468 at 5075.109 kb on + strand, within PP_4468 at 5075.109 kb on + strand, within PP_4468 at 5075.109 kb on + strand, within PP_4468 at 5075.109 kb on + strand, within PP_4468 at 5075.110 kb on - strand, within PP_4468 at 5075.110 kb on - strand at 5075.110 kb on - strand, within PP_4468 at 5075.110 kb on - strand, within PP_4468 at 5075.110 kb on - strand, within PP_4468 at 5075.140 kb on + strand, within PP_4468 at 5075.140 kb on + strand, within PP_4468 at 5075.176 kb on - strand, within PP_4468 at 5075.234 kb on + strand at 5075.234 kb on + strand at 5075.264 kb on + strand at 5075.264 kb on + strand at 5075.264 kb on + strand at 5075.265 kb on - strand at 5075.346 kb on + strand at 5075.347 kb on - strand at 5075.347 kb on - strand at 5075.347 kb on - strand at 5075.364 kb on - strand at 5075.395 kb on + strand at 5075.395 kb on + strand at 5075.395 kb on + strand at 5075.396 kb on - strand at 5075.396 kb on - strand at 5075.404 kb on + strand at 5075.404 kb on + strand at 5075.405 kb on - strand at 5075.405 kb on - strand at 5075.405 kb on - strand at 5075.434 kb on + strand at 5075.584 kb on + strand, within PP_t58 at 5075.585 kb on - strand, within PP_t58 at 5075.619 kb on + strand, within PP_t58 at 5075.619 kb on + strand, within PP_t58 at 5075.619 kb on + strand, within PP_t58 at 5075.662 kb on + strand at 5075.663 kb on - strand at 5075.663 kb on - strand at 5075.717 kb on + strand at 5075.717 kb on + strand at 5075.739 kb on + strand at 5075.739 kb on + strand at 5075.739 kb on + strand at 5075.739 kb on + strand at 5075.739 kb on + strand at 5075.739 kb on + strand at 5075.739 kb on + strand at 5075.740 kb on - strand at 5075.740 kb on - strand at 5075.740 kb on - strand at 5075.747 kb on + strand at 5075.747 kb on + strand at 5075.757 kb on + strand at 5075.758 kb on - strand at 5075.771 kb on + strand, within PP_t57 at 5075.771 kb on + strand, within PP_t57 at 5075.771 kb on + strand, within PP_t57 at 5075.772 kb on - strand, within PP_t57 at 5075.772 kb on - strand, within PP_t57 at 5075.772 kb on - strand, within PP_t57 at 5075.823 kb on - strand, within PP_t57 at 5075.834 kb on - strand at 5075.876 kb on + strand at 5075.877 kb on - strand at 5075.923 kb on + strand at 5075.924 kb on - strand at 5075.953 kb on + strand at 5075.954 kb on - strand at 5075.957 kb on - strand at 5075.957 kb on - strand at 5076.018 kb on + strand, within PP_4469 at 5076.200 kb on + strand, within PP_4469 at 5076.272 kb on + strand, within PP_4469 at 5076.272 kb on + strand, within PP_4469 at 5076.273 kb on - strand, within PP_4469 at 5076.273 kb on - strand, within PP_4469 at 5076.273 kb on - strand, within PP_4469 at 5076.273 kb on - strand, within PP_4469 at 5076.277 kb on + strand, within PP_4469 at 5076.277 kb on + strand, within PP_4469 at 5076.278 kb on - strand, within PP_4469 at 5076.278 kb on - strand, within PP_4469 at 5076.327 kb on + strand, within PP_4469 at 5076.488 kb on - strand at 5076.488 kb on - strand at 5076.534 kb on + strand at 5076.541 kb on + strand at 5076.541 kb on + strand at 5076.542 kb on - strand at 5076.542 kb on - strand at 5076.542 kb on - strand at 5076.548 kb on + strand at 5076.548 kb on + strand at 5076.548 kb on + strand at 5076.549 kb on - strand at 5076.549 kb on - strand at 5076.551 kb on - strand at 5076.636 kb on + strand, within PP_5693 at 5076.705 kb on - strand, within PP_5693 at 5076.716 kb on + strand, within PP_5693 at 5076.717 kb on - strand, within PP_5693 at 5076.727 kb on + strand, within PP_5693 at 5076.728 kb on - strand, within PP_5693 at 5076.728 kb on - strand, within PP_5693 at 5076.728 kb on - strand, within PP_5693 at 5076.728 kb on - strand, within PP_5693 at 5076.815 kb on + strand at 5076.816 kb on - strand at 5076.877 kb on - strand at 5076.877 kb on - strand at 5076.899 kb on + strand at 5076.903 kb on + strand at 5076.904 kb on - strand at 5076.974 kb on - strand, within PP_4470 at 5077.035 kb on - strand, within PP_4470 at 5077.035 kb on - strand, within PP_4470 at 5077.235 kb on + strand at 5077.235 kb on + strand at 5077.235 kb on + strand at 5077.236 kb on - strand at 5077.236 kb on - strand at 5077.236 kb on - strand at 5077.314 kb on + strand at 5077.314 kb on + strand at 5077.314 kb on + strand at 5077.315 kb on - strand at 5077.463 kb on + strand, within PP_4471 at 5077.518 kb on + strand, within PP_4471 at 5077.518 kb on + strand, within PP_4471 at 5077.518 kb on + strand at 5077.519 kb on - strand, within PP_4471 at 5077.519 kb on - strand, within PP_4471
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,074,983 + PP_4468 0.48 -0.2 5,074,983 + PP_4468 0.48 -0.5 5,075,037 + PP_4468 0.59 +1.2 5,075,037 + PP_4468 0.59 -1.1 5,075,038 - PP_4468 0.60 +1.9 5,075,067 + PP_4468 0.65 -1.2 5,075,067 + +2.9 5,075,067 + PP_4468 0.65 -0.4 5,075,067 + PP_4468 0.65 +1.2 5,075,067 + PP_4468 0.65 -2.0 5,075,068 - PP_4468 0.66 +0.1 5,075,068 - PP_4468 0.66 +1.0 5,075,099 - PP_4468 0.72 -0.1 5,075,099 - PP_4468 0.72 +0.1 5,075,099 - PP_4468 0.72 -0.0 5,075,109 + PP_4468 0.74 +0.6 5,075,109 + PP_4468 0.74 +2.6 5,075,109 + PP_4468 0.74 -1.0 5,075,109 + PP_4468 0.74 +1.6 5,075,109 + PP_4468 0.74 +2.2 5,075,109 + PP_4468 0.74 +0.7 5,075,110 - PP_4468 0.74 +1.1 5,075,110 - -0.2 5,075,110 - PP_4468 0.74 +0.5 5,075,110 - PP_4468 0.74 +1.0 5,075,110 - PP_4468 0.74 +0.1 5,075,140 + PP_4468 0.80 +2.3 5,075,140 + PP_4468 0.80 -0.3 5,075,176 - PP_4468 0.88 -1.2 5,075,234 + -0.9 5,075,234 + -1.1 5,075,264 + +0.5 5,075,264 + -0.7 5,075,264 + +0.1 5,075,265 - +1.9 5,075,346 + -0.4 5,075,347 - +0.4 5,075,347 - +1.1 5,075,347 - -0.8 5,075,364 - -0.3 5,075,395 + +0.2 5,075,395 + +0.4 5,075,395 + +0.2 5,075,396 - -0.6 5,075,396 - -0.2 5,075,404 + -0.3 5,075,404 + +0.1 5,075,405 - -0.4 5,075,405 - -0.6 5,075,405 - -0.3 5,075,434 + -0.2 5,075,584 + PP_t58 0.39 -0.4 5,075,585 - PP_t58 0.40 -0.6 5,075,619 + PP_t58 0.88 -0.1 5,075,619 + PP_t58 0.88 +1.9 5,075,619 + PP_t58 0.88 +1.9 5,075,662 + -1.4 5,075,663 - -0.2 5,075,663 - -0.5 5,075,717 + -0.0 5,075,717 + +0.9 5,075,739 + +0.8 5,075,739 + -1.6 5,075,739 + +0.6 5,075,739 + +0.5 5,075,739 + +1.2 5,075,739 + -0.5 5,075,739 + -0.4 5,075,740 - -0.3 5,075,740 - -0.6 5,075,740 - -0.0 5,075,747 + +0.6 5,075,747 + -0.6 5,075,757 + -0.9 5,075,758 - -1.0 5,075,771 + PP_t57 0.13 -0.3 5,075,771 + PP_t57 0.13 +0.3 5,075,771 + PP_t57 0.13 +0.4 5,075,772 - PP_t57 0.14 -1.3 5,075,772 - PP_t57 0.14 +0.1 5,075,772 - PP_t57 0.14 -0.6 5,075,823 - PP_t57 0.88 +1.3 5,075,834 - -0.3 5,075,876 + -1.8 5,075,877 - -2.1 5,075,923 + -2.0 5,075,924 - -0.7 5,075,953 + -1.1 5,075,954 - +1.5 5,075,957 - +0.7 5,075,957 - -0.0 5,076,018 + PP_4469 0.13 -0.0 5,076,200 + PP_4469 0.43 -1.9 5,076,272 + PP_4469 0.55 +0.3 5,076,272 + PP_4469 0.55 +2.9 5,076,273 - PP_4469 0.55 -0.4 5,076,273 - PP_4469 0.55 +0.1 5,076,273 - PP_4469 0.55 -0.1 5,076,273 - PP_4469 0.55 -2.6 5,076,277 + PP_4469 0.56 -0.1 5,076,277 + PP_4469 0.56 -0.9 5,076,278 - PP_4469 0.56 -0.7 5,076,278 - PP_4469 0.56 -2.0 5,076,327 + PP_4469 0.64 -0.4 5,076,488 - +0.8 5,076,488 - -1.7 5,076,534 + -0.1 5,076,541 + +0.4 5,076,541 + +0.2 5,076,542 - -0.4 5,076,542 - +0.4 5,076,542 - +2.9 5,076,548 + +0.3 5,076,548 + +1.6 5,076,548 + -0.6 5,076,549 - +0.2 5,076,549 - +0.0 5,076,551 - -0.7 5,076,636 + PP_5693 0.36 -1.0 5,076,705 - PP_5693 0.61 +0.1 5,076,716 + PP_5693 0.65 +0.0 5,076,717 - PP_5693 0.66 -0.8 5,076,727 + PP_5693 0.69 -0.6 5,076,728 - PP_5693 0.70 +0.6 5,076,728 - PP_5693 0.70 +0.5 5,076,728 - PP_5693 0.70 +1.4 5,076,728 - PP_5693 0.70 -0.3 5,076,815 + +1.1 5,076,816 - +0.1 5,076,877 - -0.9 5,076,877 - +0.6 5,076,899 + -0.3 5,076,903 + -0.4 5,076,904 - +0.0 5,076,974 - PP_4470 0.29 +0.9 5,077,035 - PP_4470 0.48 +0.6 5,077,035 - PP_4470 0.48 +0.2 5,077,235 + +0.2 5,077,235 + +0.2 5,077,235 + +0.1 5,077,236 - +0.5 5,077,236 - -0.6 5,077,236 - -0.2 5,077,314 + +0.6 5,077,314 + -0.3 5,077,314 + +2.3 5,077,315 - -0.4 5,077,463 + PP_4471 0.10 +3.9 5,077,518 + PP_4471 0.14 -0.7 5,077,518 + PP_4471 0.14 -0.9 5,077,518 + +2.9 5,077,519 - PP_4471 0.14 +0.9 5,077,519 - PP_4471 0.14 +3.9
Or see this region's nucleotide sequence