Strain Fitness in Pseudomonas putida KT2440 around PP_4174
Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
| Position | Strand | Gene | LocusTag | Fraction | 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. |
|---|---|---|---|---|---|
| remove | |||||
| 4,715,938 | + | PP_4173 | 0.60 | +1.0 | |
| 4,716,124 | - | PP_4173 | 0.70 | +0.9 | |
| 4,716,187 | + | PP_4173 | 0.73 | +1.8 | |
| 4,716,188 | - | PP_4173 | 0.73 | +0.9 | |
| 4,716,188 | - | PP_4173 | 0.73 | +1.6 | |
| 4,716,290 | - | PP_4173 | 0.79 | +0.1 | |
| 4,716,319 | + | PP_4173 | 0.80 | +0.5 | |
| 4,716,320 | - | PP_4173 | 0.80 | +0.1 | |
| 4,716,391 | + | PP_4173 | 0.84 | +0.3 | |
| 4,716,391 | + | PP_4173 | 0.84 | +1.3 | |
| 4,716,391 | + | PP_4173 | 0.84 | +0.7 | |
| 4,716,391 | + | PP_4173 | 0.84 | +1.0 | |
| 4,716,392 | - | PP_4173 | 0.84 | +1.4 | |
| 4,716,392 | - | PP_4173 | 0.84 | -0.1 | |
| 4,716,490 | - | PP_4173 | 0.89 | +0.9 | |
| 4,716,490 | - | PP_4173 | 0.89 | +0.9 | |
| 4,716,610 | - | +2.9 | |||
| 4,716,820 | + | +1.5 |
Or see this region's nucleotide sequence