Strain Fitness in Pseudomonas putida KT2440 around PP_3240

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,674,875	+				-0.6
3,674,875	+				+2.9
3,674,875	+				-0.3
3,674,875	+				+0.2
3,674,875	+				-0.2
3,674,876	-				-1.0
3,674,876	-				-0.4
3,674,879	+				-1.6
3,675,418	+		PP_3239	0.64	-0.5
3,675,419	-		PP_3239	0.64	+0.5
3,675,462	-		PP_3239	0.68	-0.7
3,675,806	-				-0.1
3,675,970	-		PP_3240	0.85	+1.1
3,675,991	+				-1.2
3,675,992	-				-1.1
3,676,009	-				+0.7
3,676,113	-		PP_5592	0.11	+0.6
3,676,181	-		PP_5592	0.38	-0.5
3,676,248	+		PP_5592	0.65	+0.5
3,676,249	-		PP_5592	0.65	-0.9
3,676,249	-		PP_5592	0.65	-0.7
3,676,271	+		PP_5592	0.74	-1.1
3,676,272	-		PP_5592	0.74	+0.2
3,676,303	+		PP_5592	0.87	+0.1
3,676,307	+		PP_5592	0.88	-2.0
3,676,308	-		PP_5592	0.88	+0.5
3,676,308	-		PP_5592	0.88	-0.7
3,676,308	-		PP_5592	0.88	+0.6
3,676,308	-		PP_5592	0.88	-0.3
3,676,308	-		PP_5592	0.88	-0.1
3,676,330	+				-0.4
3,676,330	+				+0.6
3,676,330	+				-0.3
3,676,330	+				+1.8
3,676,330	+				-0.6
3,676,511	+		PP_3241	0.33	-1.7
3,676,839	+				-0.8
3,676,839	+				-0.3
3,676,840	-				+0.2
3,676,840	-				-1.1
3,676,858	+				+0.3
3,676,959	-				-1.0
3,676,959	-				-0.5
3,676,959	-				+0.4
3,676,959	-				-0.4
3,676,960	+				-0.7
3,676,961	-				+1.6