Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2824 and PP_2825 are separated by 69 nucleotides PP_2825 and PP_2826 are separated by 33 nucleotides PP_2826 and PP_2827 are separated by 206 nucleotides
PP_2824: PP_2824 - Transcriptional regulator, TetR family, at 3,223,253 to 3,223,933
_2824
PP_2825: PP_2825 - putative Integrase, at 3,224,003 to 3,224,470
_2825
PP_2826: PP_2826 - Transcriptional regulator MexT, at 3,224,504 to 3,225,418
_2826
PP_2827: PP_2827 - Alcohol dehydrogenase, zinc-containing, at 3,225,625 to 3,226,647
_2827
Position (kb)
3224
3225
3226 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3223.600 kb on + strand, within PP_2824 at 3223.601 kb on - strand, within PP_2824 at 3223.710 kb on + strand, within PP_2824 at 3223.711 kb on - strand, within PP_2824 at 3223.711 kb on - strand, within PP_2824 at 3223.922 kb on + strand at 3223.934 kb on - strand at 3223.961 kb on + strand at 3223.961 kb on + strand at 3223.962 kb on - strand at 3224.030 kb on + strand at 3224.129 kb on + strand, within PP_2825 at 3224.130 kb on - strand, within PP_2825 at 3224.130 kb on - strand, within PP_2825 at 3224.130 kb on - strand, within PP_2825 at 3224.131 kb on + strand, within PP_2825 at 3224.132 kb on - strand, within PP_2825 at 3224.132 kb on - strand at 3224.204 kb on - strand, within PP_2825 at 3224.210 kb on + strand, within PP_2825 at 3224.316 kb on - strand, within PP_2825 at 3224.405 kb on - strand, within PP_2825 at 3224.512 kb on + strand at 3224.534 kb on - strand at 3224.677 kb on - strand, within PP_2826 at 3224.677 kb on - strand, within PP_2826 at 3224.952 kb on + strand, within PP_2826 at 3224.953 kb on - strand, within PP_2826 at 3225.006 kb on + strand, within PP_2826 at 3225.433 kb on + strand at 3225.433 kb on + strand at 3225.433 kb on + strand at 3225.434 kb on - strand at 3225.456 kb on + strand at 3225.457 kb on - strand at 3225.472 kb on + strand at 3225.543 kb on + strand at 3225.594 kb on + strand at 3225.598 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 3,223,600 + PP_2824 0.51 +0.7 3,223,601 - PP_2824 0.51 +0.7 3,223,710 + PP_2824 0.67 +0.3 3,223,711 - PP_2824 0.67 -1.1 3,223,711 - PP_2824 0.67 -0.2 3,223,922 + -0.6 3,223,934 - -0.5 3,223,961 + +0.4 3,223,961 + +0.4 3,223,962 - +0.7 3,224,030 + -0.7 3,224,129 + PP_2825 0.27 -1.1 3,224,130 - PP_2825 0.27 +0.5 3,224,130 - PP_2825 0.27 +0.6 3,224,130 - PP_2825 0.27 +2.9 3,224,131 + PP_2825 0.27 +0.2 3,224,132 - PP_2825 0.28 -1.8 3,224,132 - -0.3 3,224,204 - PP_2825 0.43 +0.0 3,224,210 + PP_2825 0.44 +0.4 3,224,316 - PP_2825 0.67 +1.1 3,224,405 - PP_2825 0.86 -1.2 3,224,512 + -0.3 3,224,534 - -1.4 3,224,677 - PP_2826 0.19 -0.6 3,224,677 - PP_2826 0.19 -1.1 3,224,952 + PP_2826 0.49 -1.3 3,224,953 - PP_2826 0.49 +0.7 3,225,006 + PP_2826 0.55 -2.6 3,225,433 + +0.1 3,225,433 + -0.4 3,225,433 + -1.9 3,225,434 - +0.2 3,225,456 + +0.4 3,225,457 - +1.9 3,225,472 + +0.3 3,225,543 + +0.5 3,225,594 + +1.9 3,225,598 + +0.5
Or see this region's nucleotide sequence