Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2785 and PP_2786 are separated by 50 nucleotides PP_2786 and PP_2787 are separated by 33 nucleotides
PP_2785: PP_2785 - conserved exported protein of unknown function, at 3,172,496 to 3,173,779
_2785
PP_2786: PP_2786 - conserved exported protein of unknown function, at 3,173,830 to 3,174,666
_2786
PP_2787: PP_2787 - putative Transporter, at 3,174,700 to 3,177,309
_2787
Position (kb)
3173
3174
3175 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3172.884 kb on + strand, within PP_2785 at 3172.884 kb on + strand, within PP_2785 at 3173.654 kb on + strand at 3173.769 kb on - strand at 3174.025 kb on + strand, within PP_2786 at 3174.026 kb on - strand, within PP_2786 at 3174.067 kb on + strand, within PP_2786 at 3174.068 kb on - strand, within PP_2786 at 3174.158 kb on - strand, within PP_2786 at 3174.229 kb on + strand, within PP_2786 at 3174.235 kb on + strand, within PP_2786 at 3174.269 kb on - strand, within PP_2786 at 3174.269 kb on - strand, within PP_2786 at 3174.316 kb on + strand, within PP_2786 at 3174.434 kb on + strand, within PP_2786 at 3174.435 kb on - strand, within PP_2786 at 3174.501 kb on + strand, within PP_2786 at 3174.501 kb on + strand, within PP_2786 at 3174.501 kb on + strand, within PP_2786 at 3174.568 kb on + strand, within PP_2786 at 3174.568 kb on + strand, within PP_2786 at 3174.647 kb on - strand at 3174.710 kb on - strand at 3174.710 kb on - strand at 3174.772 kb on + strand at 3174.782 kb on - strand at 3174.818 kb on - strand at 3174.887 kb on - strand at 3174.887 kb on - strand at 3174.887 kb on - strand at 3175.439 kb on - strand, within PP_2787 at 3175.468 kb on + strand, within PP_2787 at 3175.469 kb on - strand, within PP_2787 at 3175.656 kb on + strand, within PP_2787 at 3175.656 kb on + strand, within PP_2787
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 3,172,884 + PP_2785 0.30 +0.7 3,172,884 + PP_2785 0.30 -1.3 3,173,654 + -0.4 3,173,769 - -1.1 3,174,025 + PP_2786 0.23 -0.5 3,174,026 - PP_2786 0.23 -0.7 3,174,067 + PP_2786 0.28 +0.2 3,174,068 - PP_2786 0.28 -0.4 3,174,158 - PP_2786 0.39 -0.1 3,174,229 + PP_2786 0.48 -0.8 3,174,235 + PP_2786 0.48 +0.9 3,174,269 - PP_2786 0.52 +3.9 3,174,269 - PP_2786 0.52 +0.1 3,174,316 + PP_2786 0.58 -0.2 3,174,434 + PP_2786 0.72 +0.4 3,174,435 - PP_2786 0.72 -0.2 3,174,501 + PP_2786 0.80 -0.3 3,174,501 + PP_2786 0.80 +2.9 3,174,501 + PP_2786 0.80 -0.3 3,174,568 + PP_2786 0.88 +0.1 3,174,568 + PP_2786 0.88 -1.9 3,174,647 - +0.3 3,174,710 - +3.9 3,174,710 - +0.3 3,174,772 + +0.2 3,174,782 - -0.4 3,174,818 - +0.0 3,174,887 - +0.3 3,174,887 - +1.0 3,174,887 - +1.0 3,175,439 - PP_2787 0.28 -0.8 3,175,468 + PP_2787 0.29 +2.9 3,175,469 - PP_2787 0.29 -0.0 3,175,656 + PP_2787 0.37 +0.5 3,175,656 + PP_2787 0.37 +0.0
Or see this region's nucleotide sequence