Strain Fitness in Pseudomonas putida KT2440 around PP_2692
Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
| Position | Strand | Gene | LocusTag | Fraction | 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. |
|---|---|---|---|---|---|
| remove | |||||
| 3,081,692 | + | PP_2691 | 0.52 | -0.7 | |
| 3,081,775 | + | PP_2691 | 0.57 | -1.3 | |
| 3,081,975 | - | PP_2691 | 0.69 | +0.6 | |
| 3,081,982 | + | PP_2691 | 0.69 | -0.9 | |
| 3,082,794 | - | PP_2692 | 0.18 | -0.8 | |
| 3,082,827 | - | PP_2692 | 0.20 | -0.8 | |
| 3,083,029 | - | PP_2692 | 0.33 | -0.9 | |
| 3,083,050 | - | PP_2692 | 0.34 | -3.2 | |
| 3,083,070 | + | PP_2692 | 0.36 | -0.0 | |
| 3,083,283 | - | PP_2692 | 0.49 | -1.0 | |
| 3,083,283 | - | PP_2692 | 0.49 | -0.2 | |
| 3,083,290 | - | PP_2692 | 0.50 | -0.2 | |
| 3,083,332 | + | PP_2692 | 0.53 | -2.2 | |
| 3,083,332 | + | PP_2692 | 0.53 | -1.0 | |
| 3,083,529 | + | PP_2692 | 0.65 | -0.3 | |
| 3,083,529 | + | PP_2692 | 0.65 | -0.8 | |
| 3,083,801 | + | PP_2692 | 0.83 | +0.1 | |
| 3,083,801 | + | PP_2692 | 0.83 | -0.6 | |
| 3,083,802 | - | PP_2692 | 0.83 | -0.6 | |
| 3,083,872 | - | PP_2692 | 0.87 | -0.1 | |
| 3,084,307 | + | PP_2693 | 0.19 | -0.0 | |
| 3,084,308 | - | PP_2693 | 0.19 | +0.1 | |
| 3,084,737 | - | PP_2693 | 0.62 | +0.6 | |
| 3,084,769 | + | PP_2693 | 0.65 | +1.0 | |
| 3,084,867 | - | PP_2693 | 0.75 | +0.7 | |
| 3,085,053 | + | -0.2 | |||
| 3,085,054 | - | -1.0 | |||
| 3,085,054 | - | +0.8 | |||
| 3,085,055 | + | -1.2 | |||
| 3,085,056 | - | -1.6 |
Or see this region's nucleotide sequence