Strain Fitness in Pseudomonas putida KT2440 around PP_2643

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,027,437	+		PP_2642	0.54	+0.0
3,027,437	+		PP_2642	0.54	-0.4
3,027,437	+		PP_2642	0.54	+0.3
3,027,437	+		PP_2642	0.54	-0.8
3,027,438	-		PP_2642	0.54	-0.9
3,027,438	-		PP_2642	0.54	-1.1
3,027,438	-		PP_2642	0.54	+0.6
3,027,438	-		PP_2642	0.54	+0.5
3,027,442	-		PP_2642	0.54	-0.6
3,027,442	-		PP_2642	0.54	+2.0
3,027,548	+		PP_2642	0.61	-0.3
3,027,549	-		PP_2642	0.61	+0.4
3,027,555	-		PP_2642	0.62	-0.0
3,027,581	+		PP_2642	0.63	+0.7
3,027,671	+		PP_2642	0.69	+0.4
3,027,671	+		PP_2642	0.69	+2.0
3,027,671	+		PP_2642	0.69	+0.1
3,027,672	-		PP_2642	0.69	+0.7
3,027,672	-		PP_2642	0.69	-0.2
3,027,673	+		PP_2642	0.70	+0.3
3,027,674	-		PP_2642	0.70	+0.8
3,028,038	+				+0.4
3,028,102	+				-1.1
3,028,102	+				-1.9
3,028,103	-				-1.1
3,028,198	+				-1.3
3,028,211	+				-0.3
3,028,212	-				+0.1
3,028,219	-				-0.2
3,028,304	+				+1.1
3,028,305	-				+2.9
3,028,365	+				+0.2
3,028,365	+				+0.1
3,028,366	-				+0.5
3,028,366	-				-0.7
3,028,366	-				-0.5
3,028,807	+		PP_2643	0.28	-1.3
3,028,807	+		PP_2643	0.28	-1.2
3,028,807	+		PP_2643	0.28	-0.7
3,028,808	-		PP_2643	0.28	-0.8
3,028,808	-		PP_2643	0.28	+0.2
3,029,097	+		PP_2643	0.45	-0.9
3,029,782	+		PP_2643	0.87	-1.0
3,029,783	-		PP_2643	0.87	-1.7
3,030,029	+				+0.7
3,030,076	+				-0.5
3,030,150	+				+1.1
3,030,150	+				-0.5
3,030,151	-				-0.5
3,030,419	-				-0.6
3,030,478	-				+1.5
3,030,539	+				-1.9
3,030,554	-				-0.3
3,030,754	+				-0.2
3,030,754	+				+0.7
3,030,759	+				-0.5
3,030,759	+				-1.0
3,030,759	+				+0.1
3,030,991	+		PP_2645	0.11	-0.1
3,030,991	+		PP_2645	0.11	+0.7
3,030,991	+		PP_2645	0.11	+0.9
3,030,991	+		PP_2645	0.11	+1.0
3,030,991	+		PP_2645	0.11	-0.4
3,030,992	-		PP_2645	0.11	+0.5
3,030,992	-		PP_2645	0.11	-0.8
3,030,992	-		PP_2645	0.11	+0.2
3,030,992	-		PP_2645	0.11	-1.2
3,030,992	-		PP_2645	0.11	-0.3
3,030,992	-		PP_2645	0.11	-1.0
3,030,992	-		PP_2645	0.11	+1.6
3,030,995	+		PP_2645	0.11	-0.9
3,030,996	-		PP_2645	0.11	-0.9
3,030,996	-		PP_2645	0.11	+1.6
3,030,996	-		PP_2645	0.11	-0.4
3,030,996	-		PP_2645	0.11	+0.7
3,030,996	-		PP_2645	0.11	+0.8