Strain Fitness in Pseudomonas putida KT2440 around PP_2436

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,781,653	+		PP_2435	0.41	-0.9
2,781,653	+		PP_2435	0.41	+2.3
2,781,654	-		PP_2435	0.42	+0.2
2,781,654	-		PP_2435	0.42	-0.5
2,781,654	-		PP_2435	0.42	+0.1
2,781,681	-		PP_2435	0.44	+0.5
2,781,723	+		PP_2435	0.47	-0.8
2,781,723	+		PP_2435	0.47	-0.5
2,782,045	-		PP_2435	0.74	-1.5
2,782,090	+		PP_2435	0.77	+0.4
2,782,090	+		PP_2435	0.77	-0.2
2,782,263	+				-1.3
2,782,283	+				+0.3
2,782,284	-				+0.2
2,782,353	-				+0.1
2,782,474	+				-0.7
2,782,475	-				-0.3
2,782,608	+		PP_2436	0.12	-0.9
2,782,608	+		PP_2436	0.12	+0.5
2,782,737	+		PP_2436	0.26	-0.6
2,782,738	-		PP_2436	0.26	-2.0
2,782,801	-		PP_2436	0.33	+2.0
2,782,801	-		PP_2436	0.33	-0.8
2,782,828	+		PP_2436	0.36	-2.4
2,782,828	+		PP_2436	0.36	-0.3
2,782,829	-		PP_2436	0.36	-0.3
2,782,829	-		PP_2436	0.36	-0.1
2,782,829	-		PP_2436	0.36	+0.6
2,782,852	-		PP_2436	0.38	-2.1
2,782,911	+		PP_2436	0.45	-0.7
2,782,911	+		PP_2436	0.45	-0.9
2,783,326	+		PP_2436	0.90	+1.6
2,783,327	-		PP_2436	0.90	-0.0
2,783,371	-				+0.2
2,783,413	+				-0.6
2,783,414	-				+0.4
2,783,414	-				+1.0
2,783,414	-				-0.3
2,783,458	+				-0.2
2,783,459	-				+0.1
2,783,497	+				-1.0
2,783,521	+				-0.0
2,783,522	-				-0.8
2,783,537	+				+0.3
2,783,537	+				+0.6
2,783,555	+				-0.1
2,783,712	+		PP_2437	0.13	+1.1
2,783,713	-		PP_2437	0.13	-0.2
2,783,713	-		PP_2437	0.13	+1.2
2,783,767	+		PP_2437	0.18	+0.6
2,783,794	+		PP_2437	0.20	-0.4
2,784,414	+		PP_2437	0.70	+0.9
2,784,415	-		PP_2437	0.70	-0.0
2,784,415	-		PP_2437	0.70	-0.2