Strain Fitness in Pseudomonas putida KT2440 around PP_1874

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,096,185	+		PP_1872	0.76	-0.3
2,096,185	+		PP_1872	0.76	+0.7
2,096,186	-		PP_1872	0.76	-0.3
2,096,186	-		PP_1872	0.76	+0.5
2,096,186	-		PP_1872	0.76	-1.3
2,096,186	-		PP_1872	0.76	+0.9
2,096,213	+		PP_1872	0.78	-0.6
2,096,214	-		PP_1872	0.78	+0.2
2,096,278	+		PP_1872	0.83	-0.5
2,096,279	-				+1.9
2,096,319	+		PP_1872	0.87	-1.1
2,096,437	+				+1.1
2,096,437	+				-1.1
2,096,437	+				-0.1
2,096,438	-				-0.1
2,096,505	+				-0.2
2,096,505	+				-1.0
2,096,576	+				+0.7
2,096,576	+				-0.2
2,096,576	+				-1.1
2,096,577	-				-2.0
2,096,577	-				+0.6
2,096,579	+				-0.7
2,096,579	+				-0.8
2,096,579	+				+0.1
2,096,579	+				-0.3
2,096,579	+				+0.1
2,096,580	-				-0.2
2,096,580	-				+0.7
2,096,580	-				+0.5
2,096,581	+				+0.7
2,096,581	+				-0.0
2,096,581	+				+1.5
2,096,581	+				-0.7
2,096,581	+				-0.7
2,096,581	+				-0.2
2,096,581	+				-1.0
2,096,582	-				+0.3
2,096,582	-				-1.3
2,096,582	-				-0.8
2,096,582	-				+0.1
2,096,582	-				-1.6
2,096,582	-				-1.3
2,096,582	-				-0.7
2,096,582	-				-0.3
2,096,582	-				-0.1
2,096,642	+				-0.6
2,096,642	+				-1.5
2,096,642	+				+0.4
2,096,642	+				+0.6
2,096,642	+				+0.7
2,096,642	+				-1.5
2,096,643	-				+0.1
2,096,643	-				+1.0
2,096,643	-				-2.4
2,096,643	-				+0.1
2,096,703	-		PP_1873	0.15	+0.7
2,096,709	+		PP_1873	0.16	-0.4
2,096,750	+		PP_1873	0.27	+0.4
2,096,750	+		PP_1873	0.27	+1.0
2,096,751	-		PP_1873	0.27	+0.4
2,096,751	-		PP_1873	0.27	-1.2
2,096,777	+		PP_1873	0.33	+1.2
2,096,777	+		PP_1873	0.33	-0.7
2,096,778	-		PP_1873	0.34	-0.9
2,096,778	-		PP_1873	0.34	-0.5
2,096,778	-		PP_1873	0.34	-0.0
2,096,856	-		PP_1873	0.53	+0.0
2,096,856	-		PP_1873	0.53	-1.3
2,097,180	+		PP_1874	0.26	-0.6
2,097,180	+		PP_1874	0.26	-1.0
2,097,180	+		PP_1874	0.26	+0.2
2,097,181	-		PP_1874	0.26	-1.5
2,097,181	-		PP_1874	0.26	-0.7
2,097,211	-		PP_1874	0.32	+0.7
2,097,233	-		PP_1874	0.37	-0.9
2,097,308	+		PP_1874	0.52	+0.1
2,097,404	+		PP_1874	0.72	-0.8
2,097,480	+		PP_1874	0.87	-1.1
2,097,481	-		PP_1874	0.87	-1.1
2,097,571	+				-0.3
2,097,571	+				+2.5
2,097,571	+				-0.2
2,097,602	+				-0.4
2,097,603	-				+0.6
2,097,884	-		PP_1875	0.15	-0.0
2,097,884	-		PP_1875	0.15	-0.3
2,097,884	-		PP_1875	0.15	+0.2
2,097,892	+		PP_1875	0.15	-1.0
2,097,893	-		PP_1875	0.15	+2.9
2,098,194	-		PP_1875	0.28	-0.3
2,098,194	-		PP_1875	0.28	+0.1
2,098,523	-		PP_1875	0.42	+0.2
2,098,523	-		PP_1875	0.42	-1.1