Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1833 and PP_1834 are separated by 236 nucleotides PP_1834 and PP_1835 are separated by 60 nucleotides PP_1835 and PP_1836 are separated by 123 nucleotides
PP_1833: PP_1833 - conserved protein of unknown function, at 2,057,912 to 2,058,214
_1833
PP_1834: PP_1834 - conserved protein of unknown function, at 2,058,451 to 2,058,975
_1834
PP_1835: PP_1835 - conserved protein of unknown function, at 2,059,036 to 2,059,566
_1835
PP_1836: PP_1836 - putative metal transporter, ZIP family, at 2,059,690 to 2,060,628
_1836
Position (kb)
2059
2060 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2058.051 kb on + strand, within PP_1833 at 2058.051 kb on + strand, within PP_1833 at 2058.098 kb on - strand, within PP_1833 at 2058.104 kb on + strand, within PP_1833 at 2058.104 kb on + strand, within PP_1833 at 2058.130 kb on + strand, within PP_1833 at 2058.184 kb on + strand at 2058.267 kb on + strand at 2058.377 kb on + strand at 2058.377 kb on + strand at 2058.377 kb on + strand at 2058.377 kb on + strand at 2058.377 kb on + strand at 2058.433 kb on + strand at 2058.433 kb on + strand at 2058.434 kb on - strand at 2058.434 kb on - strand at 2058.450 kb on + strand at 2058.450 kb on + strand at 2058.451 kb on - strand at 2058.451 kb on - strand at 2058.451 kb on - strand at 2058.591 kb on + strand, within PP_1834 at 2058.591 kb on + strand, within PP_1834 at 2058.629 kb on - strand, within PP_1834 at 2058.629 kb on - strand, within PP_1834 at 2058.854 kb on - strand, within PP_1834 at 2059.133 kb on - strand, within PP_1835 at 2059.177 kb on + strand, within PP_1835 at 2059.177 kb on + strand, within PP_1835 at 2059.178 kb on - strand, within PP_1835 at 2059.179 kb on - strand, within PP_1835 at 2059.262 kb on - strand, within PP_1835 at 2059.262 kb on - strand, within PP_1835 at 2059.262 kb on - strand, within PP_1835 at 2059.262 kb on - strand, within PP_1835 at 2059.262 kb on - strand, within PP_1835 at 2059.315 kb on - strand, within PP_1835 at 2059.368 kb on + strand, within PP_1835 at 2059.369 kb on - strand, within PP_1835 at 2059.411 kb on + strand, within PP_1835 at 2059.411 kb on + strand, within PP_1835 at 2059.411 kb on + strand, within PP_1835 at 2059.411 kb on + strand, within PP_1835 at 2059.412 kb on - strand, within PP_1835 at 2059.412 kb on - strand, within PP_1835 at 2059.412 kb on - strand, within PP_1835 at 2059.412 kb on - strand, within PP_1835 at 2059.412 kb on - strand, within PP_1835 at 2059.491 kb on - strand, within PP_1835 at 2059.567 kb on + strand at 2059.610 kb on - strand at 2059.634 kb on + strand at 2059.895 kb on - strand, within PP_1836 at 2060.238 kb on + strand, within PP_1836 at 2060.238 kb on + strand, within PP_1836 at 2060.506 kb on + strand, within PP_1836 at 2060.506 kb on + strand, within PP_1836
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 2,058,051 + PP_1833 0.46 -0.8 2,058,051 + PP_1833 0.46 -1.0 2,058,098 - PP_1833 0.61 +0.6 2,058,104 + PP_1833 0.63 +0.5 2,058,104 + PP_1833 0.63 +0.7 2,058,130 + PP_1833 0.72 -0.1 2,058,184 + +1.3 2,058,267 + -1.8 2,058,377 + -1.5 2,058,377 + +0.9 2,058,377 + +0.0 2,058,377 + -0.0 2,058,377 + +0.7 2,058,433 + +0.1 2,058,433 + -0.8 2,058,434 - -1.0 2,058,434 - -1.7 2,058,450 + -0.6 2,058,450 + -1.2 2,058,451 - +0.9 2,058,451 - -0.7 2,058,451 - +1.5 2,058,591 + PP_1834 0.27 +0.1 2,058,591 + PP_1834 0.27 -0.9 2,058,629 - PP_1834 0.34 -0.7 2,058,629 - PP_1834 0.34 -0.8 2,058,854 - PP_1834 0.77 -1.8 2,059,133 - PP_1835 0.18 +0.3 2,059,177 + PP_1835 0.27 +0.6 2,059,177 + PP_1835 0.27 -0.3 2,059,178 - PP_1835 0.27 -0.1 2,059,179 - PP_1835 0.27 +2.9 2,059,262 - PP_1835 0.43 -0.5 2,059,262 - PP_1835 0.43 +1.5 2,059,262 - PP_1835 0.43 -0.4 2,059,262 - PP_1835 0.43 -1.9 2,059,262 - PP_1835 0.43 +0.6 2,059,315 - PP_1835 0.53 +1.9 2,059,368 + PP_1835 0.63 +0.8 2,059,369 - PP_1835 0.63 +2.3 2,059,411 + PP_1835 0.71 -0.0 2,059,411 + PP_1835 0.71 +0.3 2,059,411 + PP_1835 0.71 +3.9 2,059,411 + PP_1835 0.71 -0.0 2,059,412 - PP_1835 0.71 +0.3 2,059,412 - PP_1835 0.71 +0.6 2,059,412 - PP_1835 0.71 +0.3 2,059,412 - PP_1835 0.71 +0.3 2,059,412 - PP_1835 0.71 +0.2 2,059,491 - PP_1835 0.86 -0.7 2,059,567 + -1.0 2,059,610 - +1.3 2,059,634 + +0.1 2,059,895 - PP_1836 0.22 -0.9 2,060,238 + PP_1836 0.58 -0.0 2,060,238 + PP_1836 0.58 -0.9 2,060,506 + PP_1836 0.87 -0.8 2,060,506 + PP_1836 0.87 -0.2
Or see this region's nucleotide sequence