Strain Fitness in Pseudomonas putida KT2440 around PP_1502

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1500 and PP_1501 overlap by 4 nucleotidesPP_1501 and PP_1502 are separated by 141 nucleotidesPP_1502 and PP_1503 are separated by 50 nucleotidesPP_1503 and PP_1504 are separated by 219 nucleotides PP_1500: PP_1500 - putative Lipoprotein, at 1,705,936 to 1,706,859 _1500 PP_1501: PP_1501 - conserved protein of unknown function, at 1,706,856 to 1,707,182 _1501 PP_1502: PP_1502 - OmpA family protein, at 1,707,324 to 1,708,115 _1502 PP_1503: PP_1503 - conserved protein of unknown function, at 1,708,166 to 1,708,636 _1503 PP_1504: PP_1504 - conserved protein of unknown function, at 1,708,856 to 1,709,233 _1504 Position (kb) 1707 1708 1709Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1706.381 kb on + strand, within PP_1500at 1706.381 kb on + strand, within PP_1500at 1706.382 kb on - strand, within PP_1500at 1706.422 kb on + strand, within PP_1500at 1706.423 kb on - strand, within PP_1500at 1706.491 kb on + strand, within PP_1500at 1706.680 kb on + strand, within PP_1500at 1706.680 kb on + strand, within PP_1500at 1706.680 kb on + strand, within PP_1500at 1706.681 kb on - strand, within PP_1500at 1706.751 kb on + strand, within PP_1500at 1706.751 kb on + strand, within PP_1500at 1706.751 kb on + strand, within PP_1500at 1706.751 kb on + strand, within PP_1500at 1706.752 kb on - strand, within PP_1500at 1706.934 kb on + strand, within PP_1501at 1706.935 kb on - strand, within PP_1501at 1706.937 kb on + strand, within PP_1501at 1706.937 kb on + strand, within PP_1501at 1706.937 kb on + strand, within PP_1501at 1706.938 kb on - strand, within PP_1501at 1707.022 kb on - strand, within PP_1501at 1707.022 kb on - strand, within PP_1501at 1707.022 kb on - strand, within PP_1501at 1707.022 kb on - strand, within PP_1501at 1707.028 kb on + strand, within PP_1501at 1707.547 kb on + strand, within PP_1502at 1707.547 kb on + strand, within PP_1502at 1707.548 kb on - strand, within PP_1502at 1707.548 kb on - strand, within PP_1502at 1707.574 kb on + strand, within PP_1502at 1707.574 kb on + strand, within PP_1502at 1707.574 kb on + strand, within PP_1502at 1707.574 kb on + strand, within PP_1502at 1707.656 kb on + strand, within PP_1502at 1707.828 kb on - strand, within PP_1502at 1707.828 kb on - strand, within PP_1502at 1707.878 kb on - strandat 1707.920 kb on - strand, within PP_1502at 1707.920 kb on - strand, within PP_1502at 1708.102 kb on - strandat 1708.115 kb on + strandat 1708.115 kb on + strandat 1708.115 kb on + strandat 1708.116 kb on - strandat 1708.116 kb on - strandat 1708.116 kb on - strandat 1708.116 kb on - strandat 1708.196 kb on + strandat 1708.197 kb on - strandat 1708.443 kb on + strand, within PP_1503at 1708.444 kb on - strand, within PP_1503at 1708.444 kb on - strand, within PP_1503at 1708.489 kb on + strand, within PP_1503at 1708.490 kb on - strand, within PP_1503at 1708.550 kb on - strand, within PP_1503at 1708.610 kb on - strandat 1708.636 kb on + strandat 1708.726 kb on + strandat 1708.727 kb on - strandat 1708.758 kb on + strandat 1708.758 kb on + strandat 1708.759 kb on - strandat 1708.759 kb on - strandat 1708.931 kb on - strand, within PP_1504at 1708.948 kb on - strand, within PP_1504at 1708.994 kb on + strand, within PP_1504at 1708.994 kb on + strand, within PP_1504at 1708.994 kb on + strand, within PP_1504at 1708.994 kb on + strand, within PP_1504at 1708.994 kb on + strand, within PP_1504at 1708.994 kb on + strand, within PP_1504at 1708.994 kb on + strand, within PP_1504at 1708.995 kb on - strandat 1708.995 kb on - strand, within PP_1504at 1708.995 kb on - strand, within PP_1504at 1708.995 kb on - strand, within PP_1504at 1708.998 kb on + strand, within PP_1504at 1708.998 kb on + strand, within PP_1504at 1708.998 kb on + strand, within PP_1504at 1708.998 kb on + strand, within PP_1504at 1708.998 kb on + strand, within PP_1504at 1708.998 kb on + strand, within PP_1504at 1708.998 kb on + strand, within PP_1504at 1709.076 kb on + strand, within PP_1504at 1709.077 kb on - strand, within PP_1504

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Per-strain Table

Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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1,706,381 + PP_1500 0.48 -0.0
1,706,381 + PP_1500 0.48 -0.5
1,706,382 - PP_1500 0.48 +0.1
1,706,422 + PP_1500 0.53 +0.3
1,706,423 - PP_1500 0.53 +1.9
1,706,491 + PP_1500 0.60 +0.5
1,706,680 + PP_1500 0.81 +0.7
1,706,680 + PP_1500 0.81 -0.4
1,706,680 + PP_1500 0.81 +0.0
1,706,681 - PP_1500 0.81 -0.5
1,706,751 + PP_1500 0.88 -0.1
1,706,751 + PP_1500 0.88 -1.8
1,706,751 + PP_1500 0.88 -0.4
1,706,751 + PP_1500 0.88 -0.2
1,706,752 - PP_1500 0.88 -0.0
1,706,934 + PP_1501 0.24 -0.6
1,706,935 - PP_1501 0.24 +0.1
1,706,937 + PP_1501 0.25 -0.7
1,706,937 + PP_1501 0.25 +0.3
1,706,937 + PP_1501 0.25 +1.7
1,706,938 - PP_1501 0.25 +0.1
1,707,022 - PP_1501 0.51 -1.1
1,707,022 - PP_1501 0.51 -0.0
1,707,022 - PP_1501 0.51 -0.3
1,707,022 - PP_1501 0.51 +0.0
1,707,028 + PP_1501 0.53 +0.5
1,707,547 + PP_1502 0.28 -0.2
1,707,547 + PP_1502 0.28 +1.2
1,707,548 - PP_1502 0.28 -0.8
1,707,548 - PP_1502 0.28 +0.6
1,707,574 + PP_1502 0.32 +0.2
1,707,574 + PP_1502 0.32 -0.6
1,707,574 + PP_1502 0.32 +0.2
1,707,574 + PP_1502 0.32 +1.0
1,707,656 + PP_1502 0.42 +0.7
1,707,828 - PP_1502 0.64 +0.7
1,707,828 - PP_1502 0.64 +0.8
1,707,878 - +3.9
1,707,920 - PP_1502 0.75 +0.1
1,707,920 - PP_1502 0.75 +1.5
1,708,102 - -0.3
1,708,115 + +0.1
1,708,115 + +1.5
1,708,115 + -2.4
1,708,116 - +0.3
1,708,116 - +0.2
1,708,116 - -0.4
1,708,116 - -2.3
1,708,196 + -1.8
1,708,197 - -1.0
1,708,443 + PP_1503 0.59 -0.1
1,708,444 - PP_1503 0.59 +0.5
1,708,444 - PP_1503 0.59 -0.3
1,708,489 + PP_1503 0.69 -1.1
1,708,490 - PP_1503 0.69 +2.0
1,708,550 - PP_1503 0.82 -0.3
1,708,610 - -1.2
1,708,636 + +0.2
1,708,726 + -0.1
1,708,727 - -0.9
1,708,758 + +0.6
1,708,758 + +0.6
1,708,759 - +0.3
1,708,759 - -0.2
1,708,931 - PP_1504 0.20 +0.3
1,708,948 - PP_1504 0.24 +0.0
1,708,994 + PP_1504 0.37 -0.8
1,708,994 + PP_1504 0.37 +0.2
1,708,994 + PP_1504 0.37 -0.5
1,708,994 + PP_1504 0.37 +0.8
1,708,994 + PP_1504 0.37 -1.1
1,708,994 + PP_1504 0.37 -1.4
1,708,994 + PP_1504 0.37 -0.1
1,708,995 - +1.9
1,708,995 - PP_1504 0.37 -0.0
1,708,995 - PP_1504 0.37 -0.5
1,708,995 - PP_1504 0.37 +3.3
1,708,998 + PP_1504 0.38 -0.0
1,708,998 + PP_1504 0.38 +0.3
1,708,998 + PP_1504 0.38 -0.9
1,708,998 + PP_1504 0.38 +0.0
1,708,998 + PP_1504 0.38 -0.2
1,708,998 + PP_1504 0.38 +0.1
1,708,998 + PP_1504 0.38 -1.2
1,709,076 + PP_1504 0.58 +1.2
1,709,077 - PP_1504 0.58 -1.6

Or see this region's nucleotide sequence