Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1347 and PP_1348 overlap by 4 nucleotides PP_1348 and PP_1349 are separated by 32 nucleotides PP_1349 and PP_1350 are separated by 1 nucleotides
PP_1347: PP_1347 - Glutathione S-transferase family protein, at 1,536,365 to 1,536,997
_1347
PP_1348: PP_1348 - putative MutT/nudix family protein/thiamine-phosphate pyrophosphorylase, at 1,536,994 to 1,537,938
_1348
PP_1349: PP_1349 - ATP:cob(I)alamin adenosyltransferase, at 1,537,971 to 1,538,537
_1349
PP_1350: PP_1350 - Sensory box histidine kinase/response regulator, at 1,538,539 to 1,540,569
_1350
Position (kb) 1537
1538
1539 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1536.988 kb on + strand at 1536.989 kb on - strand at 1536.989 kb on - strand at 1537.010 kb on + strand at 1537.010 kb on + strand at 1537.011 kb on - strand at 1537.011 kb on - strand at 1537.013 kb on + strand at 1537.178 kb on + strand, within PP_1348 at 1537.388 kb on - strand at 1537.388 kb on - strand, within PP_1348 at 1537.492 kb on + strand, within PP_1348 at 1537.492 kb on + strand, within PP_1348 at 1537.492 kb on + strand, within PP_1348 at 1537.492 kb on + strand, within PP_1348 at 1537.493 kb on - strand, within PP_1348 at 1537.493 kb on - strand, within PP_1348 at 1537.493 kb on - strand, within PP_1348 at 1537.493 kb on - strand, within PP_1348 at 1537.493 kb on - strand, within PP_1348 at 1537.504 kb on + strand, within PP_1348 at 1537.636 kb on + strand, within PP_1348 at 1537.637 kb on - strand, within PP_1348 at 1537.904 kb on + strand at 1537.966 kb on + strand at 1537.966 kb on + strand at 1537.966 kb on + strand at 1537.967 kb on - strand at 1537.967 kb on - strand at 1537.969 kb on + strand at 1537.970 kb on - strand at 1537.970 kb on - strand at 1537.970 kb on - strand at 1538.074 kb on - strand, within PP_1349 at 1538.075 kb on + strand, within PP_1349 at 1538.076 kb on - strand, within PP_1349 at 1538.076 kb on - strand, within PP_1349 at 1538.076 kb on - strand, within PP_1349 at 1538.077 kb on + strand, within PP_1349 at 1538.078 kb on - strand, within PP_1349 at 1538.078 kb on - strand, within PP_1349 at 1538.078 kb on - strand, within PP_1349 at 1538.288 kb on - strand, within PP_1349 at 1538.512 kb on + strand at 1538.512 kb on + strand at 1538.513 kb on - strand at 1538.513 kb on - strand at 1538.513 kb on - strand at 1538.513 kb on - strand at 1538.513 kb on - strand at 1538.513 kb on - strand at 1538.513 kb on - strand at 1538.513 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 1,536,988 + +0.7 1,536,989 - -0.7 1,536,989 - -1.1 1,537,010 + +0.7 1,537,010 + -0.0 1,537,011 - +0.7 1,537,011 - +0.5 1,537,013 + +1.3 1,537,178 + PP_1348 0.19 +0.1 1,537,388 - -1.9 1,537,388 - PP_1348 0.42 -1.6 1,537,492 + PP_1348 0.53 +3.9 1,537,492 + PP_1348 0.53 -3.2 1,537,492 + PP_1348 0.53 +0.6 1,537,492 + PP_1348 0.53 -2.3 1,537,493 - PP_1348 0.53 -1.1 1,537,493 - PP_1348 0.53 -2.4 1,537,493 - PP_1348 0.53 -0.8 1,537,493 - PP_1348 0.53 -2.3 1,537,493 - PP_1348 0.53 -0.5 1,537,504 + PP_1348 0.54 +0.7 1,537,636 + PP_1348 0.68 -1.2 1,537,637 - PP_1348 0.68 -0.1 1,537,904 + -1.1 1,537,966 + -0.0 1,537,966 + +0.9 1,537,966 + -0.1 1,537,967 - -0.3 1,537,967 - +0.2 1,537,969 + +0.9 1,537,970 - -0.3 1,537,970 - +0.6 1,537,970 - -1.3 1,538,074 - PP_1349 0.18 -0.6 1,538,075 + PP_1349 0.18 -0.7 1,538,076 - PP_1349 0.19 -0.3 1,538,076 - PP_1349 0.19 -1.0 1,538,076 - PP_1349 0.19 +0.4 1,538,077 + PP_1349 0.19 -1.5 1,538,078 - PP_1349 0.19 -2.0 1,538,078 - PP_1349 0.19 +0.1 1,538,078 - PP_1349 0.19 -1.7 1,538,288 - PP_1349 0.56 -1.4 1,538,512 + +0.5 1,538,512 + -0.5 1,538,513 - -0.6 1,538,513 - -1.4 1,538,513 - +0.7 1,538,513 - +0.0 1,538,513 - -0.8 1,538,513 - +3.7 1,538,513 - -1.3 1,538,513 - -0.4
Or see this region's nucleotide sequence