Strain Fitness in Pseudomonas putida KT2440 around PP_1261

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1260 and PP_1261 are separated by 431 nucleotidesPP_1261 and PP_1262 are separated by 175 nucleotides PP_1260: PP_1260 - transposase, at 1,440,328 to 1,441,656 _1260 PP_1261: PP_1261 - 2-ketoaldonate reductase / hydroxypyruvate/glyoxylate reductase, at 1,442,088 to 1,443,062 _1261 PP_1262: PP_1262 - LysR family transcriptional regulator, at 1,443,238 to 1,444,185 _1262 Position (kb) 1442 1443 1444Strain fitness (log2 ratio) -1 0 1at 1442.039 kb on + strandat 1442.824 kb on + strand, within PP_1261at 1442.825 kb on - strand, within PP_1261at 1442.825 kb on - strand, within PP_1261at 1442.834 kb on - strand, within PP_1261at 1443.127 kb on - strandat 1443.148 kb on + strandat 1443.149 kb on - strandat 1443.205 kb on + strandat 1443.206 kb on - strandat 1443.351 kb on - strand, within PP_1262at 1443.366 kb on - strand, within PP_1262at 1443.374 kb on + strand, within PP_1262at 1443.374 kb on + strand, within PP_1262at 1443.374 kb on + strand, within PP_1262at 1443.374 kb on + strand, within PP_1262at 1443.375 kb on - strand, within PP_1262at 1443.375 kb on - strand, within PP_1262at 1443.409 kb on + strand, within PP_1262at 1443.410 kb on - strand, within PP_1262at 1443.424 kb on - strand, within PP_1262at 1443.452 kb on + strand, within PP_1262at 1443.452 kb on + strand, within PP_1262at 1443.452 kb on + strand, within PP_1262at 1443.452 kb on + strand, within PP_1262at 1443.671 kb on + strand, within PP_1262at 1443.698 kb on + strand, within PP_1262at 1443.698 kb on + strand, within PP_1262at 1443.699 kb on - strand, within PP_1262at 1443.699 kb on - strand, within PP_1262at 1443.699 kb on - strand, within PP_1262at 1443.771 kb on - strand, within PP_1262at 1443.962 kb on + strand, within PP_1262at 1443.966 kb on + strand, within PP_1262at 1443.966 kb on + strand, within PP_1262at 1443.966 kb on + strand, within PP_1262at 1443.966 kb on + strand, within PP_1262at 1443.967 kb on - strand, within PP_1262at 1443.992 kb on + strand, within PP_1262at 1444.033 kb on - strand, within PP_1262

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Per-strain Table

Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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1,442,039 + +1.0
1,442,824 + PP_1261 0.75 -1.4
1,442,825 - PP_1261 0.76 -0.8
1,442,825 - PP_1261 0.76 -0.9
1,442,834 - PP_1261 0.77 -1.3
1,443,127 - +0.3
1,443,148 + +0.5
1,443,149 - +1.7
1,443,205 + -0.0
1,443,206 - +0.3
1,443,351 - PP_1262 0.12 -1.7
1,443,366 - PP_1262 0.14 +0.5
1,443,374 + PP_1262 0.14 +0.1
1,443,374 + PP_1262 0.14 +1.5
1,443,374 + PP_1262 0.14 +0.7
1,443,374 + PP_1262 0.14 +0.2
1,443,375 - PP_1262 0.14 -1.1
1,443,375 - PP_1262 0.14 +0.8
1,443,409 + PP_1262 0.18 +0.5
1,443,410 - PP_1262 0.18 +0.6
1,443,424 - PP_1262 0.20 +0.2
1,443,452 + PP_1262 0.23 +0.9
1,443,452 + PP_1262 0.23 +1.0
1,443,452 + PP_1262 0.23 +1.1
1,443,452 + PP_1262 0.23 +1.3
1,443,671 + PP_1262 0.46 +1.3
1,443,698 + PP_1262 0.49 +0.2
1,443,698 + PP_1262 0.49 +0.2
1,443,699 - PP_1262 0.49 +0.3
1,443,699 - PP_1262 0.49 -0.4
1,443,699 - PP_1262 0.49 -0.3
1,443,771 - PP_1262 0.56 +0.9
1,443,962 + PP_1262 0.76 +1.1
1,443,966 + PP_1262 0.77 -0.3
1,443,966 + PP_1262 0.77 +0.6
1,443,966 + PP_1262 0.77 -1.0
1,443,966 + PP_1262 0.77 +0.6
1,443,967 - PP_1262 0.77 +0.1
1,443,992 + PP_1262 0.80 +0.4
1,444,033 - PP_1262 0.84 +0.3

Or see this region's nucleotide sequence