Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0662 and PP_0663 overlap by 4 nucleotides PP_0663 and PP_0664 are separated by 198 nucleotides PP_0664 and PP_0665 are separated by 12 nucleotides
PP_0662: PP_0662 - putative Threonine synthase, at 770,317 to 771,705
_0662
PP_0663: PP_0663 - Transcriptional regulator, AsnC family, at 771,702 to 772,139
_0663
PP_0664: PP_0664 - homoserine dehydrogenase, at 772,338 to 773,381
_0664
PP_0665: PP_0665 - putative glyceraldehyde-3-phosphate dehydrogenase, at 773,394 to 774,812
_0665
Position (kb) 772
773
774 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 771.394 kb on + strand, within PP_0662 at 771.395 kb on - strand, within PP_0662 at 771.461 kb on + strand, within PP_0662 at 771.485 kb on + strand, within PP_0662 at 771.486 kb on - strand, within PP_0662 at 771.863 kb on + strand, within PP_0663 at 771.863 kb on + strand, within PP_0663 at 771.864 kb on - strand, within PP_0663 at 771.864 kb on - strand, within PP_0663 at 771.864 kb on - strand, within PP_0663 at 771.924 kb on + strand, within PP_0663 at 771.924 kb on + strand, within PP_0663 at 771.931 kb on - strand, within PP_0663 at 772.159 kb on + strand at 772.249 kb on + strand at 772.250 kb on - strand at 772.254 kb on - strand at 772.254 kb on - strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.294 kb on - strand at 772.298 kb on + strand at 772.299 kb on - strand at 772.299 kb on - strand at 772.788 kb on + strand, within PP_0664 at 772.827 kb on - strand, within PP_0664 at 772.862 kb on - strand, within PP_0664 at 772.886 kb on + strand, within PP_0664 at 772.886 kb on + strand, within PP_0664 at 772.887 kb on - strand, within PP_0664 at 772.892 kb on + strand, within PP_0664 at 772.892 kb on + strand, within PP_0664 at 772.893 kb on - strand, within PP_0664 at 772.893 kb on - strand, within PP_0664 at 772.893 kb on - strand, within PP_0664 at 772.896 kb on + strand, within PP_0664 at 772.896 kb on + strand, within PP_0664 at 772.896 kb on + strand, within PP_0664 at 772.896 kb on + strand, within PP_0664 at 772.896 kb on + strand, within PP_0664 at 772.896 kb on + strand, within PP_0664 at 772.897 kb on - strand, within PP_0664 at 772.897 kb on - strand, within PP_0664 at 772.897 kb on - strand, within PP_0664 at 772.897 kb on - strand, within PP_0664 at 773.008 kb on + strand, within PP_0664 at 773.008 kb on + strand, within PP_0664 at 773.008 kb on + strand, within PP_0664 at 773.009 kb on - strand, within PP_0664 at 773.009 kb on - strand, within PP_0664 at 773.009 kb on - strand, within PP_0664 at 773.183 kb on - strand, within PP_0664 at 773.410 kb on + strand at 773.451 kb on + strand at 773.451 kb on + strand at 773.451 kb on + strand at 773.451 kb on + strand at 773.452 kb on - strand at 773.452 kb on - strand at 773.452 kb on - strand at 773.452 kb on - strand at 773.766 kb on + strand, within PP_0665 at 773.766 kb on + strand, within PP_0665 at 773.766 kb on + strand, within PP_0665 at 773.766 kb on + strand, within PP_0665 at 773.766 kb on + strand, within PP_0665 at 773.766 kb on + strand, within PP_0665 at 773.767 kb on - strand, within PP_0665 at 773.767 kb on - strand, within PP_0665 at 773.767 kb on - strand, within PP_0665 at 773.767 kb on - strand, within PP_0665 at 773.832 kb on - strand, within PP_0665 at 773.956 kb on - strand, within PP_0665 at 773.996 kb on - strand, within PP_0665 at 774.253 kb on + strand, within PP_0665 at 774.254 kb on - strand, within PP_0665 at 774.254 kb on - strand at 774.268 kb on - strand, within PP_0665 at 774.268 kb on - strand, within PP_0665
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 771,394 + PP_0662 0.78 +1.7 771,395 - PP_0662 0.78 -2.0 771,461 + PP_0662 0.82 -1.8 771,485 + PP_0662 0.84 +0.1 771,486 - PP_0662 0.84 -0.1 771,863 + PP_0663 0.37 -2.0 771,863 + PP_0663 0.37 -0.8 771,864 - PP_0663 0.37 +0.5 771,864 - PP_0663 0.37 -0.8 771,864 - PP_0663 0.37 -1.4 771,924 + PP_0663 0.51 +1.3 771,924 + PP_0663 0.51 -0.3 771,931 - PP_0663 0.52 +0.6 772,159 + -0.6 772,249 + -1.2 772,250 - +1.6 772,254 - -0.3 772,254 - +2.2 772,293 + -0.0 772,293 + -0.9 772,293 + -1.1 772,293 + -0.2 772,293 + -2.1 772,293 + +1.3 772,294 - -0.7 772,298 + -0.5 772,299 - +0.9 772,299 - -1.0 772,788 + PP_0664 0.43 +0.4 772,827 - PP_0664 0.47 -0.9 772,862 - PP_0664 0.50 -0.7 772,886 + PP_0664 0.52 -0.5 772,886 + PP_0664 0.52 +2.1 772,887 - PP_0664 0.53 +0.1 772,892 + PP_0664 0.53 -0.4 772,892 + PP_0664 0.53 -0.5 772,893 - PP_0664 0.53 +2.9 772,893 - PP_0664 0.53 +0.5 772,893 - PP_0664 0.53 -0.0 772,896 + PP_0664 0.53 -0.6 772,896 + PP_0664 0.53 -0.6 772,896 + PP_0664 0.53 +0.7 772,896 + PP_0664 0.53 -0.0 772,896 + PP_0664 0.53 +0.2 772,896 + PP_0664 0.53 +0.0 772,897 - PP_0664 0.54 -1.4 772,897 - PP_0664 0.54 +0.2 772,897 - PP_0664 0.54 -1.2 772,897 - PP_0664 0.54 -0.6 773,008 + PP_0664 0.64 +0.1 773,008 + PP_0664 0.64 -1.0 773,008 + PP_0664 0.64 +0.2 773,009 - PP_0664 0.64 +1.1 773,009 - PP_0664 0.64 -1.0 773,009 - PP_0664 0.64 -0.9 773,183 - PP_0664 0.81 -1.3 773,410 + +1.0 773,451 + -0.6 773,451 + +2.9 773,451 + +0.1 773,451 + -0.4 773,452 - +1.2 773,452 - +0.4 773,452 - -1.4 773,452 - -0.8 773,766 + PP_0665 0.26 +0.5 773,766 + PP_0665 0.26 +0.1 773,766 + PP_0665 0.26 +0.9 773,766 + PP_0665 0.26 +1.0 773,766 + PP_0665 0.26 +0.2 773,766 + PP_0665 0.26 -0.5 773,767 - PP_0665 0.26 -3.5 773,767 - PP_0665 0.26 +1.1 773,767 - PP_0665 0.26 -0.9 773,767 - PP_0665 0.26 -0.5 773,832 - PP_0665 0.31 -0.7 773,956 - PP_0665 0.40 -0.0 773,996 - PP_0665 0.42 +0.8 774,253 + PP_0665 0.61 +0.7 774,254 - PP_0665 0.61 -1.1 774,254 - +2.3 774,268 - PP_0665 0.62 +0.3 774,268 - PP_0665 0.62 -0.3
Or see this region's nucleotide sequence