Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0570 and PP_0571 are separated by 151 nucleotides PP_0571 and PP_0572 are separated by 35 nucleotides
PP_0570: PP_0570 - lipoprotein of unknown function, at 663,135 to 668,036
_0570
PP_0571: PP_0571 - conserved membrane protein of unknown function, at 668,188 to 668,523
_0571
PP_0572: PP_0572 - peptidoglycan glycosyltransferase, at 668,559 to 670,913
_0572
Position (kb)
668
669 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 667.524 kb on + strand, within PP_0570 at 667.524 kb on + strand, within PP_0570 at 667.525 kb on - strand, within PP_0570 at 667.602 kb on + strand at 667.602 kb on + strand at 667.602 kb on + strand at 667.603 kb on - strand at 667.610 kb on + strand at 667.611 kb on - strand at 667.849 kb on - strand at 667.861 kb on + strand at 667.864 kb on - strand at 667.891 kb on - strand at 667.891 kb on - strand at 667.912 kb on - strand at 667.942 kb on - strand at 667.942 kb on - strand at 668.282 kb on + strand, within PP_0571 at 668.282 kb on + strand, within PP_0571 at 668.473 kb on + strand, within PP_0571 at 668.473 kb on + strand, within PP_0571 at 668.473 kb on + strand, within PP_0571 at 668.473 kb on + strand, within PP_0571 at 668.473 kb on + strand, within PP_0571 at 668.474 kb on - strand, within PP_0571 at 668.474 kb on - strand, within PP_0571 at 668.474 kb on - strand, within PP_0571 at 668.474 kb on - strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.481 kb on + strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.482 kb on - strand, within PP_0571 at 668.570 kb on - strand at 668.771 kb on - strand at 668.817 kb on + strand, within PP_0572 at 668.817 kb on + strand, within PP_0572 at 668.836 kb on - strand, within PP_0572 at 668.966 kb on + strand, within PP_0572 at 668.992 kb on + strand, within PP_0572 at 668.993 kb on - strand, within PP_0572 at 668.993 kb on - strand, within PP_0572 at 668.993 kb on - strand, within PP_0572 at 669.255 kb on + strand, within PP_0572 at 669.256 kb on - strand, within PP_0572 at 669.389 kb on + strand, within PP_0572 at 669.389 kb on + strand, within PP_0572 at 669.465 kb on - strand, within PP_0572 at 669.465 kb on - strand, within PP_0572 at 669.516 kb on + strand, within PP_0572 at 669.516 kb on + strand, within PP_0572
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 667,524 + PP_0570 0.90 +2.9 667,524 + PP_0570 0.90 +0.2 667,525 - PP_0570 0.90 +1.7 667,602 + +2.5 667,602 + -0.1 667,602 + -1.3 667,603 - +0.2 667,610 + -0.2 667,611 - -0.7 667,849 - -0.3 667,861 + -1.5 667,864 - +1.0 667,891 - +2.9 667,891 - +1.4 667,912 - -1.0 667,942 - +0.2 667,942 - +2.9 668,282 + PP_0571 0.28 -0.0 668,282 + PP_0571 0.28 +0.0 668,473 + PP_0571 0.85 +0.5 668,473 + PP_0571 0.85 -0.1 668,473 + PP_0571 0.85 +0.5 668,473 + PP_0571 0.85 +0.9 668,473 + PP_0571 0.85 +1.3 668,474 - PP_0571 0.85 +3.9 668,474 - PP_0571 0.85 +0.2 668,474 - PP_0571 0.85 +0.7 668,474 - PP_0571 0.85 -1.3 668,481 + PP_0571 0.87 +0.9 668,481 + PP_0571 0.87 -0.9 668,481 + PP_0571 0.87 +1.5 668,481 + PP_0571 0.87 +1.8 668,481 + PP_0571 0.87 +0.4 668,481 + PP_0571 0.87 -0.1 668,481 + PP_0571 0.87 -0.9 668,481 + PP_0571 0.87 +0.6 668,481 + PP_0571 0.87 +0.2 668,481 + PP_0571 0.87 -1.6 668,481 + +1.6 668,481 + PP_0571 0.87 -0.5 668,481 + PP_0571 0.87 -1.0 668,481 + PP_0571 0.87 +0.8 668,481 + PP_0571 0.87 +0.6 668,482 - PP_0571 0.88 -0.2 668,482 - PP_0571 0.88 -0.7 668,482 - PP_0571 0.88 +0.2 668,482 - PP_0571 0.88 -2.5 668,482 - PP_0571 0.88 +1.6 668,482 - PP_0571 0.88 +0.5 668,482 - PP_0571 0.88 -1.0 668,482 - PP_0571 0.88 +0.1 668,482 - PP_0571 0.88 +0.3 668,482 - PP_0571 0.88 -2.3 668,482 - PP_0571 0.88 +0.7 668,570 - +0.0 668,771 - -1.5 668,817 + PP_0572 0.11 -1.3 668,817 + PP_0572 0.11 +0.9 668,836 - PP_0572 0.12 -0.6 668,966 + PP_0572 0.17 +0.7 668,992 + PP_0572 0.18 -0.7 668,993 - PP_0572 0.18 +0.4 668,993 - PP_0572 0.18 -1.4 668,993 - PP_0572 0.18 -0.8 669,255 + PP_0572 0.30 +0.7 669,256 - PP_0572 0.30 +0.2 669,389 + PP_0572 0.35 +0.5 669,389 + PP_0572 0.35 -0.0 669,465 - PP_0572 0.38 +0.3 669,465 - PP_0572 0.38 +0.5 669,516 + PP_0572 0.41 -2.0 669,516 + PP_0572 0.41 +0.6
Or see this region's nucleotide sequence