Strain Fitness in Pseudomonas putida KT2440 around PP_0490

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0489 and PP_0490 overlap by 1 nucleotidesPP_0490 and PP_0491 overlap by 4 nucleotidesPP_0491 and PP_0492 overlap by 26 nucleotides PP_0489: PP_0489 - formate dehydrogenase-O major subunit, at 574,964 to 578,032 _0489 PP_0490: PP_0490 - formate dehydrogenase-O, beta subunit, at 578,032 to 578,982 _0490 PP_0491: PP_0491 - formate dehydrogenase-O, gamma subunit, at 578,979 to 579,635 _0491 PP_0492: PP_0492 - formate dehydrogenase formation protein, at 579,610 to 580,566 _0492 Position (kb) 578 579Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 577.193 kb on - strand, within PP_0489at 577.304 kb on + strand, within PP_0489at 577.304 kb on + strand, within PP_0489at 577.305 kb on - strand, within PP_0489at 577.305 kb on - strand, within PP_0489at 577.365 kb on + strand, within PP_0489at 577.365 kb on + strand, within PP_0489at 577.365 kb on + strand, within PP_0489at 577.365 kb on + strand, within PP_0489at 577.523 kb on + strand, within PP_0489at 577.523 kb on + strand, within PP_0489at 577.523 kb on + strand, within PP_0489at 577.523 kb on + strand, within PP_0489at 577.524 kb on - strand, within PP_0489at 577.652 kb on + strand, within PP_0489at 577.653 kb on - strand, within PP_0489at 577.653 kb on - strand, within PP_0489at 577.667 kb on + strand, within PP_0489at 577.749 kb on + strandat 577.823 kb on - strandat 577.940 kb on + strandat 577.941 kb on - strandat 577.941 kb on - strandat 577.951 kb on + strandat 577.951 kb on + strandat 577.951 kb on + strandat 577.952 kb on - strandat 578.124 kb on + strandat 578.212 kb on + strand, within PP_0490at 578.212 kb on + strand, within PP_0490at 578.213 kb on - strand, within PP_0490at 578.213 kb on - strand, within PP_0490at 578.466 kb on + strand, within PP_0490at 578.466 kb on + strand, within PP_0490at 578.491 kb on + strand, within PP_0490at 578.491 kb on + strandat 578.492 kb on - strand, within PP_0490at 578.492 kb on - strand, within PP_0490at 578.642 kb on - strand, within PP_0490at 578.696 kb on - strand, within PP_0490at 578.696 kb on - strand, within PP_0490at 578.728 kb on + strand, within PP_0490at 578.728 kb on + strand, within PP_0490at 578.845 kb on + strand, within PP_0490at 578.845 kb on + strand, within PP_0490at 578.846 kb on - strand, within PP_0490at 578.846 kb on - strand, within PP_0490at 578.938 kb on + strandat 578.938 kb on + strandat 578.938 kb on + strandat 578.938 kb on + strandat 578.938 kb on + strandat 578.938 kb on + strandat 578.939 kb on - strandat 578.939 kb on - strandat 579.006 kb on + strandat 579.007 kb on - strandat 579.023 kb on + strandat 579.023 kb on + strandat 579.024 kb on - strandat 579.024 kb on - strandat 579.196 kb on + strand, within PP_0491at 579.196 kb on + strand, within PP_0491at 579.196 kb on + strand, within PP_0491at 579.197 kb on - strand, within PP_0491at 579.197 kb on - strand, within PP_0491at 579.303 kb on + strand, within PP_0491at 579.303 kb on + strand, within PP_0491at 579.304 kb on - strand, within PP_0491at 579.304 kb on - strand, within PP_0491at 579.304 kb on - strand, within PP_0491at 579.304 kb on - strand, within PP_0491at 579.391 kb on - strand, within PP_0491at 579.391 kb on - strand, within PP_0491at 579.489 kb on + strand, within PP_0491at 579.489 kb on + strand, within PP_0491at 579.490 kb on - strand, within PP_0491at 579.490 kb on - strand, within PP_0491at 579.650 kb on + strandat 579.650 kb on + strandat 579.651 kb on - strandat 579.651 kb on - strandat 579.779 kb on - strand, within PP_0492at 579.779 kb on - strand, within PP_0492at 579.801 kb on - strand, within PP_0492at 579.827 kb on + strand, within PP_0492at 579.956 kb on + strand, within PP_0492at 579.957 kb on - strand, within PP_0492

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Per-strain Table

Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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577,193 - PP_0489 0.73 +0.3
577,304 + PP_0489 0.76 +1.8
577,304 + PP_0489 0.76 +0.2
577,305 - PP_0489 0.76 +0.1
577,305 - PP_0489 0.76 -0.3
577,365 + PP_0489 0.78 -0.7
577,365 + PP_0489 0.78 -0.4
577,365 + PP_0489 0.78 -0.5
577,365 + PP_0489 0.78 +0.8
577,523 + PP_0489 0.83 -0.7
577,523 + PP_0489 0.83 -1.7
577,523 + PP_0489 0.83 -0.2
577,523 + PP_0489 0.83 +0.8
577,524 - PP_0489 0.83 -2.3
577,652 + PP_0489 0.88 +1.5
577,653 - PP_0489 0.88 -0.0
577,653 - PP_0489 0.88 -0.9
577,667 + PP_0489 0.88 -0.2
577,749 + -0.9
577,823 - -0.3
577,940 + +1.3
577,941 - -0.6
577,941 - +0.2
577,951 + +0.5
577,951 + -0.4
577,951 + -0.8
577,952 - -1.1
578,124 + -0.3
578,212 + PP_0490 0.19 -0.4
578,212 + PP_0490 0.19 +0.0
578,213 - PP_0490 0.19 +0.4
578,213 - PP_0490 0.19 -0.8
578,466 + PP_0490 0.46 -0.6
578,466 + PP_0490 0.46 -1.3
578,491 + PP_0490 0.48 -1.6
578,491 + +1.9
578,492 - PP_0490 0.48 -0.5
578,492 - PP_0490 0.48 -2.5
578,642 - PP_0490 0.64 +0.0
578,696 - PP_0490 0.70 -1.6
578,696 - PP_0490 0.70 -0.2
578,728 + PP_0490 0.73 +2.3
578,728 + PP_0490 0.73 -1.3
578,845 + PP_0490 0.85 -0.9
578,845 + PP_0490 0.85 -1.5
578,846 - PP_0490 0.86 +0.8
578,846 - PP_0490 0.86 +2.3
578,938 + -2.4
578,938 + -1.7
578,938 + -0.7
578,938 + +1.5
578,938 + -2.0
578,938 + -1.1
578,939 - -0.3
578,939 - +0.6
579,006 + +3.9
579,007 - +0.4
579,023 + -0.4
579,023 + -0.2
579,024 - -0.5
579,024 - -0.1
579,196 + PP_0491 0.33 +0.7
579,196 + PP_0491 0.33 +0.3
579,196 + PP_0491 0.33 +1.3
579,197 - PP_0491 0.33 +0.3
579,197 - PP_0491 0.33 +1.1
579,303 + PP_0491 0.49 +1.7
579,303 + PP_0491 0.49 -0.9
579,304 - PP_0491 0.49 -0.1
579,304 - PP_0491 0.49 +0.4
579,304 - PP_0491 0.49 +1.5
579,304 - PP_0491 0.49 -0.5
579,391 - PP_0491 0.63 +0.0
579,391 - PP_0491 0.63 -2.5
579,489 + PP_0491 0.78 +0.7
579,489 + PP_0491 0.78 +3.9
579,490 - PP_0491 0.78 -0.0
579,490 - PP_0491 0.78 -0.1
579,650 + +1.3
579,650 + -0.7
579,651 - +0.6
579,651 - -0.1
579,779 - PP_0492 0.18 -0.6
579,779 - PP_0492 0.18 +0.2
579,801 - PP_0492 0.20 -1.3
579,827 + PP_0492 0.23 +2.8
579,956 + PP_0492 0.36 -0.0
579,957 - PP_0492 0.36 -2.0

Or see this region's nucleotide sequence