Strain Fitness in Pseudomonas putida KT2440 around PP_0372

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0371 and PP_0372 are separated by 210 nucleotidesPP_0372 and PP_0373 are separated by 147 nucleotidesPP_0373 and PP_5444 are separated by 64 nucleotides PP_0371: PP_0371 - Transcriptional regulator, LysR family, at 450,949 to 451,872 _0371 PP_0372: PP_0372 - Acetylornithine aminotransferase 2, at 452,083 to 453,363 _0372 PP_0373: PP_0373 - conserved membrane protein of unknown function, at 453,511 to 453,669 _0373 PP_5444: PP_5444 - protein of unknown function, at 453,734 to 454,660 _5444 Position (kb) 452 453 454Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 451.203 kb on + strand, within PP_0371at 451.204 kb on - strand, within PP_0371at 451.204 kb on - strand, within PP_0371at 451.204 kb on - strand, within PP_0371at 451.204 kb on - strand, within PP_0371at 451.392 kb on - strand, within PP_0371at 451.392 kb on - strand, within PP_0371at 451.433 kb on + strand, within PP_0371at 451.434 kb on - strand, within PP_0371at 451.583 kb on - strand, within PP_0371at 451.583 kb on - strand, within PP_0371at 451.679 kb on + strand, within PP_0371at 451.679 kb on + strand, within PP_0371at 451.679 kb on + strand, within PP_0371at 451.680 kb on - strand, within PP_0371at 451.680 kb on - strand, within PP_0371at 451.819 kb on + strandat 451.835 kb on + strandat 451.836 kb on - strandat 451.937 kb on - strandat 451.945 kb on - strandat 451.958 kb on - strandat 452.216 kb on + strand, within PP_0372at 452.217 kb on - strand, within PP_0372at 452.266 kb on + strand, within PP_0372at 452.266 kb on + strand, within PP_0372at 452.266 kb on + strand, within PP_0372at 452.267 kb on - strand, within PP_0372at 452.267 kb on - strand, within PP_0372at 452.267 kb on - strand, within PP_0372at 452.267 kb on - strand, within PP_0372at 452.443 kb on + strand, within PP_0372at 452.444 kb on - strand, within PP_0372at 452.451 kb on + strand, within PP_0372at 452.452 kb on - strand, within PP_0372at 452.497 kb on - strand, within PP_0372at 452.538 kb on + strand, within PP_0372at 452.538 kb on + strand, within PP_0372at 452.539 kb on - strand, within PP_0372at 452.714 kb on + strand, within PP_0372at 452.860 kb on + strand, within PP_0372at 452.860 kb on + strand, within PP_0372at 453.113 kb on - strand, within PP_0372at 453.147 kb on - strand, within PP_0372at 453.240 kb on - strandat 453.240 kb on - strandat 453.240 kb on - strandat 453.240 kb on - strandat 453.477 kb on + strandat 453.478 kb on - strandat 453.478 kb on - strandat 453.478 kb on - strandat 453.489 kb on + strandat 453.489 kb on + strandat 453.519 kb on + strandat 453.597 kb on + strand, within PP_0373at 453.597 kb on + strand, within PP_0373at 453.597 kb on + strand, within PP_0373at 453.598 kb on - strand, within PP_0373at 453.598 kb on - strand, within PP_0373at 453.598 kb on - strand, within PP_0373at 453.598 kb on - strand, within PP_0373at 453.601 kb on - strand, within PP_0373at 453.622 kb on + strand, within PP_0373at 453.649 kb on + strand, within PP_0373at 453.649 kb on + strand, within PP_0373at 453.649 kb on + strand, within PP_0373at 453.650 kb on - strand, within PP_0373at 453.650 kb on - strand, within PP_0373at 453.650 kb on - strand, within PP_0373at 453.650 kb on - strand, within PP_0373at 453.650 kb on - strand, within PP_0373at 453.650 kb on - strand, within PP_0373at 453.651 kb on + strand, within PP_0373at 453.651 kb on + strand, within PP_0373at 453.652 kb on - strand, within PP_0373at 453.652 kb on - strand, within PP_0373at 453.652 kb on - strand, within PP_0373at 453.735 kb on + strandat 453.750 kb on - strandat 453.769 kb on - strandat 453.804 kb on + strandat 453.822 kb on + strandat 453.822 kb on + strandat 453.860 kb on - strand, within PP_5444at 453.860 kb on - strand, within PP_5444at 453.950 kb on - strand, within PP_5444at 454.065 kb on + strand, within PP_5444at 454.066 kb on - strand, within PP_5444at 454.066 kb on - strand, within PP_5444at 454.066 kb on - strand, within PP_5444at 454.066 kb on - strand, within PP_5444at 454.066 kb on - strand, within PP_5444at 454.066 kb on - strand, within PP_5444at 454.114 kb on - strand, within PP_5444at 454.152 kb on + strand, within PP_5444at 454.152 kb on + strandat 454.153 kb on - strand, within PP_5444at 454.153 kb on - strand, within PP_5444at 454.153 kb on - strand, within PP_5444at 454.210 kb on - strand, within PP_5444at 454.236 kb on + strand, within PP_5444at 454.236 kb on + strand, within PP_5444at 454.258 kb on + strand, within PP_5444at 454.292 kb on - strand, within PP_5444at 454.292 kb on - strand, within PP_5444at 454.340 kb on - strand, within PP_5444at 454.340 kb on - strand, within PP_5444

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Per-strain Table

Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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451,203 + PP_0371 0.27 -0.1
451,204 - PP_0371 0.28 +0.3
451,204 - PP_0371 0.28 +0.6
451,204 - PP_0371 0.28 +0.5
451,204 - PP_0371 0.28 +0.0
451,392 - PP_0371 0.48 -0.2
451,392 - PP_0371 0.48 -0.1
451,433 + PP_0371 0.52 -0.3
451,434 - PP_0371 0.52 -0.6
451,583 - PP_0371 0.69 +0.2
451,583 - PP_0371 0.69 -0.6
451,679 + PP_0371 0.79 +0.1
451,679 + PP_0371 0.79 +1.9
451,679 + PP_0371 0.79 -1.0
451,680 - PP_0371 0.79 +0.9
451,680 - PP_0371 0.79 -1.7
451,819 + +0.2
451,835 + -1.9
451,836 - +0.3
451,937 - -0.6
451,945 - -1.0
451,958 - -0.4
452,216 + PP_0372 0.10 +0.5
452,217 - PP_0372 0.10 +1.9
452,266 + PP_0372 0.14 +0.9
452,266 + PP_0372 0.14 -1.7
452,266 + PP_0372 0.14 -0.2
452,267 - PP_0372 0.14 -2.5
452,267 - PP_0372 0.14 -0.1
452,267 - PP_0372 0.14 +0.4
452,267 - PP_0372 0.14 +1.6
452,443 + PP_0372 0.28 +1.0
452,444 - PP_0372 0.28 -0.3
452,451 + PP_0372 0.29 +1.3
452,452 - PP_0372 0.29 +1.9
452,497 - PP_0372 0.32 +0.0
452,538 + PP_0372 0.36 +0.7
452,538 + PP_0372 0.36 +0.4
452,539 - PP_0372 0.36 +0.4
452,714 + PP_0372 0.49 -1.5
452,860 + PP_0372 0.61 -0.7
452,860 + PP_0372 0.61 -1.6
453,113 - PP_0372 0.80 -0.3
453,147 - PP_0372 0.83 +1.5
453,240 - +2.5
453,240 - +0.3
453,240 - +1.0
453,240 - +0.5
453,477 + +0.4
453,478 - +0.3
453,478 - +0.1
453,478 - -0.2
453,489 + -0.3
453,489 + +0.4
453,519 + -0.4
453,597 + PP_0373 0.54 -0.6
453,597 + PP_0373 0.54 -0.9
453,597 + PP_0373 0.54 +0.2
453,598 - PP_0373 0.55 +0.3
453,598 - PP_0373 0.55 -0.6
453,598 - PP_0373 0.55 +0.5
453,598 - PP_0373 0.55 -1.1
453,601 - PP_0373 0.57 -0.9
453,622 + PP_0373 0.70 +1.3
453,649 + PP_0373 0.87 -0.3
453,649 + PP_0373 0.87 +1.0
453,649 + PP_0373 0.87 -1.5
453,650 - PP_0373 0.87 -1.2
453,650 - PP_0373 0.87 -0.1
453,650 - PP_0373 0.87 +0.5
453,650 - PP_0373 0.87 +0.6
453,650 - PP_0373 0.87 -1.4
453,650 - PP_0373 0.87 +2.9
453,651 + PP_0373 0.88 +0.3
453,651 + PP_0373 0.88 -1.1
453,652 - PP_0373 0.89 +0.2
453,652 - PP_0373 0.89 +0.4
453,652 - PP_0373 0.89 +0.0
453,735 + -0.7
453,750 - +0.2
453,769 - -1.1
453,804 + +0.1
453,822 + -0.2
453,822 + +1.1
453,860 - PP_5444 0.14 -0.2
453,860 - PP_5444 0.14 +0.3
453,950 - PP_5444 0.23 +0.1
454,065 + PP_5444 0.36 +0.1
454,066 - PP_5444 0.36 -0.2
454,066 - PP_5444 0.36 +0.9
454,066 - PP_5444 0.36 -1.4
454,066 - PP_5444 0.36 -1.0
454,066 - PP_5444 0.36 +0.1
454,066 - PP_5444 0.36 -0.0
454,114 - PP_5444 0.41 -1.6
454,152 + PP_5444 0.45 -0.4
454,152 + +3.9
454,153 - PP_5444 0.45 -0.5
454,153 - PP_5444 0.45 +0.3
454,153 - PP_5444 0.45 -0.6
454,210 - PP_5444 0.51 -0.9
454,236 + PP_5444 0.54 +0.2
454,236 + PP_5444 0.54 -1.0
454,258 + PP_5444 0.57 +0.4
454,292 - PP_5444 0.60 +0.2
454,292 - PP_5444 0.60 +0.6
454,340 - PP_5444 0.65 -1.5
454,340 - PP_5444 0.65 +0.2

Or see this region's nucleotide sequence