Strain Fitness in Pseudomonas putida KT2440 around PP_0145

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0144 and PP_0145 are separated by 30 nucleotidesPP_0145 and PP_0146 are separated by 282 nucleotides PP_0144: PP_0144 - Metalloprotease, insulinase family, at 152,200 to 153,606 _0144 PP_0145: PP_0145 - Na+/Pi-cotransporter, at 153,637 to 155,295 _0145 PP_0146: PP_0146 - putative transport protein, at 155,578 to 156,375 _0146 Position (kb) 153 154 155 156Strain fitness (log2 ratio) -2 -1 0 1 2at 152.691 kb on - strand, within PP_0144at 152.723 kb on + strand, within PP_0144at 152.940 kb on - strand, within PP_0144at 153.149 kb on + strand, within PP_0144at 153.149 kb on + strand, within PP_0144at 153.150 kb on - strand, within PP_0144at 153.150 kb on - strand, within PP_0144at 153.172 kb on + strand, within PP_0144at 153.172 kb on + strand, within PP_0144at 153.172 kb on + strand, within PP_0144at 153.173 kb on - strand, within PP_0144at 153.173 kb on - strand, within PP_0144at 153.306 kb on + strand, within PP_0144at 153.306 kb on + strand, within PP_0144at 153.306 kb on + strand, within PP_0144at 153.307 kb on - strand, within PP_0144at 153.485 kb on + strandat 153.527 kb on + strandat 153.527 kb on + strandat 153.528 kb on - strandat 153.528 kb on - strandat 153.704 kb on - strandat 153.710 kb on + strandat 153.710 kb on + strandat 153.710 kb on + strandat 153.710 kb on + strandat 153.710 kb on + strandat 153.710 kb on + strandat 153.710 kb on + strandat 153.710 kb on + strandat 153.710 kb on + strandat 153.710 kb on + strandat 153.711 kb on - strandat 153.711 kb on - strandat 153.711 kb on - strandat 153.720 kb on + strandat 153.720 kb on + strandat 153.721 kb on - strandat 153.769 kb on + strandat 153.769 kb on + strandat 153.786 kb on + strandat 153.977 kb on - strand, within PP_0145at 154.142 kb on + strand, within PP_0145at 154.143 kb on - strand, within PP_0145at 154.160 kb on + strand, within PP_0145at 154.161 kb on - strand, within PP_0145at 154.381 kb on + strand, within PP_0145at 154.381 kb on + strand, within PP_0145at 154.451 kb on + strand, within PP_0145at 154.452 kb on - strand, within PP_0145at 154.452 kb on - strand, within PP_0145at 154.562 kb on + strand, within PP_0145at 154.615 kb on - strand, within PP_0145at 154.615 kb on - strand, within PP_0145at 154.615 kb on - strand, within PP_0145at 154.857 kb on + strand, within PP_0145at 155.224 kb on - strandat 155.316 kb on + strandat 155.316 kb on + strandat 155.317 kb on - strandat 155.427 kb on - strandat 155.531 kb on - strandat 155.531 kb on - strandat 155.702 kb on + strand, within PP_0146at 155.702 kb on + strand, within PP_0146at 155.702 kb on + strand, within PP_0146at 155.708 kb on + strand, within PP_0146at 155.708 kb on + strand, within PP_0146at 155.708 kb on + strand, within PP_0146at 155.708 kb on + strand, within PP_0146at 155.708 kb on + strand, within PP_0146at 155.709 kb on - strand, within PP_0146at 155.709 kb on - strand, within PP_0146at 155.709 kb on - strand, within PP_0146at 155.749 kb on + strand, within PP_0146at 155.749 kb on + strand, within PP_0146at 155.750 kb on - strand, within PP_0146at 155.754 kb on + strand, within PP_0146at 155.885 kb on - strand, within PP_0146at 155.966 kb on + strand, within PP_0146at 155.966 kb on + strand, within PP_0146at 155.966 kb on + strand, within PP_0146at 155.966 kb on + strand, within PP_0146at 155.967 kb on - strand, within PP_0146at 155.967 kb on - strand, within PP_0146at 155.967 kb on - strandat 155.967 kb on - strand, within PP_0146at 156.001 kb on + strand, within PP_0146at 156.001 kb on + strand, within PP_0146at 156.001 kb on + strand, within PP_0146at 156.002 kb on - strand, within PP_0146at 156.032 kb on + strand, within PP_0146at 156.032 kb on + strand, within PP_0146at 156.033 kb on - strand, within PP_0146at 156.033 kb on - strand, within PP_0146at 156.033 kb on - strand, within PP_0146at 156.033 kb on - strand, within PP_0146at 156.153 kb on + strand, within PP_0146at 156.153 kb on + strand, within PP_0146at 156.174 kb on + strand, within PP_0146at 156.175 kb on - strand, within PP_0146at 156.175 kb on - strand, within PP_0146

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Per-strain Table

Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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152,691 - PP_0144 0.35 -1.6
152,723 + PP_0144 0.37 -1.1
152,940 - PP_0144 0.53 +1.3
153,149 + PP_0144 0.67 -1.5
153,149 + PP_0144 0.67 +0.4
153,150 - PP_0144 0.68 +0.6
153,150 - PP_0144 0.68 -0.2
153,172 + PP_0144 0.69 -0.4
153,172 + PP_0144 0.69 +0.8
153,172 + PP_0144 0.69 +0.6
153,173 - PP_0144 0.69 +0.9
153,173 - PP_0144 0.69 -0.2
153,306 + PP_0144 0.79 +1.5
153,306 + PP_0144 0.79 -0.4
153,306 + PP_0144 0.79 +0.1
153,307 - PP_0144 0.79 -1.1
153,485 + +0.0
153,527 + -0.5
153,527 + -1.4
153,528 - -0.4
153,528 - -0.8
153,704 - +0.1
153,710 + +0.6
153,710 + -0.8
153,710 + +0.3
153,710 + -0.5
153,710 + +0.7
153,710 + +0.4
153,710 + +0.2
153,710 + +0.4
153,710 + +0.8
153,710 + +0.9
153,711 - +0.3
153,711 - -1.1
153,711 - +0.7
153,720 + +0.6
153,720 + +2.1
153,721 - +1.3
153,769 + +0.6
153,769 + +0.6
153,786 + -0.6
153,977 - PP_0145 0.20 +0.2
154,142 + PP_0145 0.30 -0.2
154,143 - PP_0145 0.31 +0.6
154,160 + PP_0145 0.32 +0.4
154,161 - PP_0145 0.32 +0.9
154,381 + PP_0145 0.45 -0.3
154,381 + PP_0145 0.45 -0.2
154,451 + PP_0145 0.49 -0.0
154,452 - PP_0145 0.49 -0.4
154,452 - PP_0145 0.49 +0.6
154,562 + PP_0145 0.56 +1.0
154,615 - PP_0145 0.59 +0.2
154,615 - PP_0145 0.59 -0.3
154,615 - PP_0145 0.59 +1.5
154,857 + PP_0145 0.74 +0.1
155,224 - -1.1
155,316 + +0.9
155,316 + -2.1
155,317 - -0.9
155,427 - -0.1
155,531 - -0.9
155,531 - -0.7
155,702 + PP_0146 0.16 +0.2
155,702 + PP_0146 0.16 -0.1
155,702 + PP_0146 0.16 -0.2
155,708 + PP_0146 0.16 -0.1
155,708 + PP_0146 0.16 +0.7
155,708 + PP_0146 0.16 -0.2
155,708 + PP_0146 0.16 +0.1
155,708 + PP_0146 0.16 +0.0
155,709 - PP_0146 0.16 -1.0
155,709 - PP_0146 0.16 +0.0
155,709 - PP_0146 0.16 -0.6
155,749 + PP_0146 0.21 +0.3
155,749 + PP_0146 0.21 -1.1
155,750 - PP_0146 0.22 +0.6
155,754 + PP_0146 0.22 +0.2
155,885 - PP_0146 0.38 -0.6
155,966 + PP_0146 0.49 +0.6
155,966 + PP_0146 0.49 -1.1
155,966 + PP_0146 0.49 -0.1
155,966 + PP_0146 0.49 +0.3
155,967 - PP_0146 0.49 +0.1
155,967 - PP_0146 0.49 +0.5
155,967 - +1.6
155,967 - PP_0146 0.49 +0.4
156,001 + PP_0146 0.53 -1.1
156,001 + PP_0146 0.53 -0.3
156,001 + PP_0146 0.53 -0.2
156,002 - PP_0146 0.53 +0.6
156,032 + PP_0146 0.57 -0.5
156,032 + PP_0146 0.57 +0.7
156,033 - PP_0146 0.57 +0.6
156,033 - PP_0146 0.57 -1.2
156,033 - PP_0146 0.57 -0.1
156,033 - PP_0146 0.57 -0.6
156,153 + PP_0146 0.72 -0.4
156,153 + PP_0146 0.72 -0.3
156,174 + PP_0146 0.75 +0.4
156,175 - PP_0146 0.75 -0.1
156,175 - PP_0146 0.75 -0.7

Or see this region's nucleotide sequence