Strain Fitness in Pseudomonas putida KT2440 around PP_0115

Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
119,336	+		PP_0114	0.51	-0.7
119,336	+		PP_0114	0.51	+0.9
119,336	+		PP_0114	0.51	+1.3
119,337	-		PP_0114	0.51	+0.3
119,453	+		PP_0114	0.63	+0.2
119,797	-				-0.1
119,803	+				-0.1
119,803	+				-0.1
119,803	+				-1.1
119,803	+				-0.6
119,803	+				+0.4
119,804	-				+1.3
119,804	-				+1.0
119,804	-				-1.7
119,804	-				-1.7
119,808	-				+0.9
119,808	-				+2.9
119,808	-				+0.6
119,830	-				+1.3
119,902	+				-1.0
119,903	-				-0.5
119,903	-				-2.3
119,919	+				+0.6
119,948	+				-0.7
119,948	+				+0.2
119,948	+				-0.4
120,001	+				+1.6
120,001	+				-0.3
120,002	-				-0.8
120,032	-				-0.6
120,367	+		PP_0115	0.15	-1.2
120,368	-		PP_0115	0.15	+0.9
120,515	-		PP_0115	0.22	+1.1
120,552	+		PP_0115	0.23	-0.8
120,552	+		PP_0115	0.23	+0.4
120,552	+		PP_0115	0.23	-0.7
120,552	+		PP_0115	0.23	+1.2
120,553	-		PP_0115	0.23	-0.1
120,553	-		PP_0115	0.23	-0.8
120,699	+		PP_0115	0.30	-0.4
120,700	-		PP_0115	0.30	+0.4
120,700	-		PP_0115	0.30	-2.7
120,700	-		PP_0115	0.30	-1.5
120,771	+		PP_0115	0.34	+1.0
120,771	+		PP_0115	0.34	-0.2
120,771	+		PP_0115	0.34	-0.5
120,911	+		PP_0115	0.40	+0.1
120,911	+		PP_0115	0.40	-0.6
121,023	-		PP_0115	0.45	-0.0
121,040	+		PP_0115	0.46	-0.9
121,136	+		PP_0115	0.51	-0.4
121,137	-		PP_0115	0.51	-1.4
121,137	-		PP_0115	0.51	+2.9
121,137	-		PP_0115	0.51	+0.1
121,153	-		PP_0115	0.51	+0.4
121,189	+		PP_0115	0.53	+0.4
121,324	+		PP_0115	0.59	-0.8
121,324	+		PP_0115	0.59	-0.9
121,325	-		PP_0115	0.59	+0.8
121,399	+		PP_0115	0.63	-1.6
121,399	+		PP_0115	0.63	-1.5
121,400	-		PP_0115	0.63	-1.7
121,400	-		PP_0115	0.63	-0.9
121,411	-		PP_0115	0.63	-0.0
121,485	+		PP_0115	0.67	-0.2
121,485	+		PP_0115	0.67	+0.3
121,486	-		PP_0115	0.67	-0.6
121,486	-		PP_0115	0.67	-0.9
121,525	+		PP_0115	0.69	-1.5
121,525	+		PP_0115	0.69	-0.5
121,525	+		PP_0115	0.69	-1.9
121,525	+		PP_0115	0.69	-0.5
121,525	+		PP_0115	0.69	-1.8
121,526	-		PP_0115	0.69	+0.3
121,526	-		PP_0115	0.69	+0.4
121,561	-		PP_0115	0.71	-1.5
121,561	-		PP_0115	0.71	-1.4
121,962	+		PP_0115	0.89	-1.0
121,962	+		PP_0115	0.89	+0.6
121,963	-		PP_0115	0.89	+0.4
122,014	+				+0.5
122,014	+				+1.3
122,014	+				-0.5
122,015	-				+1.9
122,015	-				-0.4
122,015	-				+0.6
122,015	-				-1.2
122,015	-				-0.0
122,225	+				-1.2
122,225	+				-1.5
122,225	+				+0.1
122,237	+				-0.1
122,252	+				-0.5
122,253	-				-1.0
122,404	+		PP_0116	0.22	-0.5
122,421	-		PP_0116	0.24	-0.6
122,421	-		PP_0116	0.24	+0.6
122,499	+		PP_0116	0.35	+1.0
122,500	-		PP_0116	0.35	+0.8
122,707	-		PP_0116	0.64	+2.3
122,707	-		PP_0116	0.64	-1.8
122,717	+		PP_0116	0.66	+0.4
122,718	-		PP_0116	0.66	-0.3
122,718	-		PP_0116	0.66	-1.4
122,718	-		PP_0116	0.66	-0.7
122,741	+		PP_0116	0.69	+0.2
122,741	+		PP_0116	0.69	+0.9
122,973	-				+1.9
122,997	+				+0.1
122,998	-				-0.7
122,998	-				-0.2
123,030	+				-0.6
123,030	+				+1.0
123,031	-				+0.3
123,118	+				-1.1
123,118	+				+0.8
123,119	-				+1.9
123,119	-				+0.4