Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5567 and PP_5568 are separated by 155 nucleotides PP_5568 and PP_5569 are separated by 33 nucleotides PP_5569 and PP_5571 are separated by 154 nucleotides PP_5571 and PP_5572 are separated by 8 nucleotides
PP_5567: PP_5567 - protein of unknown function, at 3,501,267 to 3,501,458
_5567
PP_5568: PP_5568 - conserved membrane protein of unknown function, at 3,501,614 to 3,502,312
_5568
PP_5569: PP_5569 - protein of unknown function of phage origin, at 3,502,346 to 3,502,549
_5569
PP_5571: PP_5571 - conserved protein of unknown function, at 3,502,704 to 3,503,015
_5571
PP_5572: PP_5572 - protein of unknown function, at 3,503,024 to 3,503,305
_5572
Position (kb)
3502
3503 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3501.405 kb on + strand, within PP_5567 at 3501.510 kb on + strand at 3501.511 kb on - strand at 3501.525 kb on - strand at 3501.770 kb on + strand, within PP_5568 at 3501.804 kb on + strand, within PP_5568 at 3501.805 kb on - strand, within PP_5568 at 3501.931 kb on + strand, within PP_5568 at 3501.957 kb on - strand, within PP_5568 at 3502.005 kb on + strand, within PP_5568 at 3502.006 kb on - strand at 3502.011 kb on + strand, within PP_5568 at 3502.097 kb on + strand, within PP_5568 at 3502.097 kb on + strand, within PP_5568 at 3502.194 kb on + strand, within PP_5568 at 3502.226 kb on + strand, within PP_5568 at 3502.247 kb on + strand at 3502.260 kb on + strand at 3502.320 kb on + strand at 3502.320 kb on + strand at 3502.392 kb on + strand, within PP_5569 at 3502.402 kb on - strand, within PP_5569 at 3502.414 kb on - strand, within PP_5569 at 3502.478 kb on - strand, within PP_5569 at 3502.478 kb on - strand at 3502.481 kb on + strand, within PP_5569 at 3502.520 kb on - strand, within PP_5569 at 3502.522 kb on - strand, within PP_5569 at 3502.593 kb on + strand at 3502.852 kb on - strand, within PP_5571 at 3503.026 kb on - strand at 3503.069 kb on - strand, within PP_5572 at 3503.154 kb on + strand, within PP_5572 at 3503.169 kb on + strand, within PP_5572 at 3503.169 kb on + strand, within PP_5572 at 3503.195 kb on - strand, within PP_5572 at 3503.427 kb on - strand at 3503.454 kb on - strand at 3503.454 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 3,501,405 + PP_5567 0.72 +4.7 3,501,510 + -0.5 3,501,511 - -0.0 3,501,525 - -0.5 3,501,770 + PP_5568 0.22 -1.8 3,501,804 + PP_5568 0.27 -0.2 3,501,805 - PP_5568 0.27 +0.4 3,501,931 + PP_5568 0.45 +1.1 3,501,957 - PP_5568 0.49 -1.9 3,502,005 + PP_5568 0.56 +1.7 3,502,006 - +2.1 3,502,011 + PP_5568 0.57 -1.8 3,502,097 + PP_5568 0.69 +1.1 3,502,097 + PP_5568 0.69 +0.3 3,502,194 + PP_5568 0.83 +0.1 3,502,226 + PP_5568 0.88 -0.4 3,502,247 + -0.1 3,502,260 + +0.2 3,502,320 + +1.2 3,502,320 + -0.3 3,502,392 + PP_5569 0.23 +3.7 3,502,402 - PP_5569 0.27 +1.0 3,502,414 - PP_5569 0.33 +0.6 3,502,478 - PP_5569 0.65 +1.5 3,502,478 - +0.1 3,502,481 + PP_5569 0.66 +1.6 3,502,520 - PP_5569 0.85 -1.4 3,502,522 - PP_5569 0.86 +0.6 3,502,593 + +2.0 3,502,852 - PP_5571 0.47 -1.1 3,503,026 - -0.1 3,503,069 - PP_5572 0.16 -0.1 3,503,154 + PP_5572 0.46 +2.7 3,503,169 + PP_5572 0.51 +0.0 3,503,169 + PP_5572 0.51 -0.5 3,503,195 - PP_5572 0.61 +0.7 3,503,427 - +0.5 3,503,454 - +2.8 3,503,454 - -0.2
Or see this region's nucleotide sequence