Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4537 and PP_4538 are separated by 255 nucleotides PP_4538 and PP_5698 are separated by 111 nucleotides
PP_4537: PP_4537 - conserved membrane protein of unknown function, at 5,153,337 to 5,154,212
_4537
PP_4538: PP_4538 - FMN-dependent NADH-azoreductase 2, at 5,154,468 to 5,155,067
_4538
PP_5698: PP_5698 - conserved exported protein of unknown function, at 5,155,179 to 5,155,493
_5698
Position (kb) 5153
5154
5155 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5152.381 kb on + strand at 5152.382 kb on - strand at 5152.427 kb on - strand at 5152.427 kb on - strand at 5152.456 kb on + strand at 5152.456 kb on + strand at 5152.456 kb on + strand at 5152.456 kb on + strand at 5152.492 kb on + strand at 5152.492 kb on + strand at 5152.492 kb on + strand at 5152.492 kb on + strand at 5152.492 kb on + strand at 5152.493 kb on - strand at 5152.495 kb on + strand at 5152.495 kb on + strand at 5152.495 kb on + strand at 5152.495 kb on + strand at 5152.496 kb on - strand at 5152.496 kb on - strand at 5152.568 kb on - strand at 5152.568 kb on - strand at 5152.756 kb on - strand at 5153.280 kb on - strand at 5153.280 kb on - strand at 5153.280 kb on - strand at 5153.551 kb on + strand, within PP_4537 at 5153.552 kb on - strand, within PP_4537 at 5153.631 kb on - strand, within PP_4537 at 5153.680 kb on + strand, within PP_4537 at 5153.680 kb on + strand, within PP_4537 at 5153.680 kb on + strand, within PP_4537 at 5153.686 kb on + strand, within PP_4537 at 5153.687 kb on - strand, within PP_4537 at 5153.702 kb on - strand, within PP_4537 at 5153.702 kb on - strand, within PP_4537 at 5153.702 kb on - strand, within PP_4537 at 5153.743 kb on + strand, within PP_4537 at 5153.744 kb on - strand, within PP_4537 at 5153.764 kb on + strand, within PP_4537 at 5153.764 kb on + strand, within PP_4537 at 5153.925 kb on + strand, within PP_4537 at 5153.925 kb on + strand, within PP_4537 at 5153.998 kb on + strand, within PP_4537 at 5153.998 kb on + strand, within PP_4537 at 5153.998 kb on + strand at 5153.998 kb on + strand, within PP_4537 at 5153.998 kb on + strand, within PP_4537 at 5153.998 kb on + strand, within PP_4537 at 5153.998 kb on + strand, within PP_4537 at 5153.998 kb on + strand, within PP_4537 at 5153.998 kb on + strand, within PP_4537 at 5153.998 kb on + strand, within PP_4537 at 5153.998 kb on + strand, within PP_4537 at 5153.999 kb on - strand, within PP_4537 at 5153.999 kb on - strand, within PP_4537 at 5153.999 kb on - strand, within PP_4537 at 5153.999 kb on - strand, within PP_4537 at 5153.999 kb on - strand, within PP_4537 at 5153.999 kb on - strand, within PP_4537 at 5153.999 kb on - strand, within PP_4537 at 5153.999 kb on - strand, within PP_4537 at 5154.010 kb on + strand, within PP_4537 at 5154.127 kb on + strand at 5154.128 kb on - strand at 5154.152 kb on - strand at 5154.244 kb on + strand at 5154.282 kb on + strand at 5154.283 kb on - strand at 5154.283 kb on - strand at 5154.335 kb on + strand at 5154.335 kb on + strand at 5154.336 kb on - strand at 5154.336 kb on - strand at 5154.336 kb on - strand at 5154.336 kb on - strand at 5154.336 kb on - strand at 5154.336 kb on - strand at 5154.336 kb on - strand at 5154.353 kb on + strand at 5154.353 kb on + strand at 5154.387 kb on + strand at 5154.403 kb on + strand at 5154.403 kb on + strand at 5154.403 kb on + strand at 5154.403 kb on + strand at 5154.404 kb on - strand at 5154.404 kb on - strand at 5154.404 kb on - strand at 5154.418 kb on + strand at 5154.418 kb on + strand at 5154.601 kb on + strand, within PP_4538 at 5154.601 kb on + strand, within PP_4538 at 5154.602 kb on - strand, within PP_4538 at 5154.602 kb on - strand, within PP_4538 at 5154.602 kb on - strand, within PP_4538 at 5154.706 kb on + strand, within PP_4538 at 5154.707 kb on - strand, within PP_4538 at 5154.707 kb on - strand, within PP_4538 at 5154.707 kb on - strand, within PP_4538 at 5154.707 kb on - strand, within PP_4538 at 5154.707 kb on - strand, within PP_4538 at 5154.778 kb on + strand, within PP_4538 at 5154.779 kb on - strand, within PP_4538 at 5154.801 kb on + strand, within PP_4538 at 5154.933 kb on + strand, within PP_4538 at 5154.933 kb on + strand, within PP_4538 at 5154.934 kb on - strand, within PP_4538 at 5154.934 kb on - strand, within PP_4538 at 5155.118 kb on + strand at 5155.119 kb on - strand at 5155.119 kb on - strand at 5155.119 kb on - strand at 5155.137 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,152,381 + -0.8 5,152,382 - -0.1 5,152,427 - -1.1 5,152,427 - -1.0 5,152,456 + -0.4 5,152,456 + -0.0 5,152,456 + -1.0 5,152,456 + +0.2 5,152,492 + -0.2 5,152,492 + +0.9 5,152,492 + +1.8 5,152,492 + +1.4 5,152,492 + +4.7 5,152,493 - -0.2 5,152,495 + +1.3 5,152,495 + -0.4 5,152,495 + +1.4 5,152,495 + +0.1 5,152,496 - +0.8 5,152,496 - +1.0 5,152,568 - +0.6 5,152,568 - +0.6 5,152,756 - -0.3 5,153,280 - -0.3 5,153,280 - -0.1 5,153,280 - +1.3 5,153,551 + PP_4537 0.24 +1.0 5,153,552 - PP_4537 0.25 -0.5 5,153,631 - PP_4537 0.34 -0.9 5,153,680 + PP_4537 0.39 +0.1 5,153,680 + PP_4537 0.39 +0.9 5,153,680 + PP_4537 0.39 -0.5 5,153,686 + PP_4537 0.40 -0.6 5,153,687 - PP_4537 0.40 +0.2 5,153,702 - PP_4537 0.42 +0.3 5,153,702 - PP_4537 0.42 +0.2 5,153,702 - PP_4537 0.42 +0.2 5,153,743 + PP_4537 0.46 -0.3 5,153,744 - PP_4537 0.46 -0.8 5,153,764 + PP_4537 0.49 +0.3 5,153,764 + PP_4537 0.49 -0.7 5,153,925 + PP_4537 0.67 +1.8 5,153,925 + PP_4537 0.67 +0.1 5,153,998 + PP_4537 0.75 -0.7 5,153,998 + PP_4537 0.75 -0.0 5,153,998 + +3.7 5,153,998 + PP_4537 0.75 +1.6 5,153,998 + PP_4537 0.75 +0.4 5,153,998 + PP_4537 0.75 -0.2 5,153,998 + PP_4537 0.75 +0.1 5,153,998 + PP_4537 0.75 +1.7 5,153,998 + PP_4537 0.75 +0.7 5,153,998 + PP_4537 0.75 +0.0 5,153,998 + PP_4537 0.75 +0.4 5,153,999 - PP_4537 0.76 -0.0 5,153,999 - PP_4537 0.76 +1.3 5,153,999 - PP_4537 0.76 +2.3 5,153,999 - PP_4537 0.76 -1.1 5,153,999 - PP_4537 0.76 -0.2 5,153,999 - PP_4537 0.76 +0.3 5,153,999 - PP_4537 0.76 +0.7 5,153,999 - PP_4537 0.76 +0.7 5,154,010 + PP_4537 0.77 +0.6 5,154,127 + +0.6 5,154,128 - -0.6 5,154,152 - +0.5 5,154,244 + -0.7 5,154,282 + -0.1 5,154,283 - +0.2 5,154,283 - +0.5 5,154,335 + +0.5 5,154,335 + -1.0 5,154,336 - -0.3 5,154,336 - -1.8 5,154,336 - +0.0 5,154,336 - +1.5 5,154,336 - +0.5 5,154,336 - -1.0 5,154,336 - -0.1 5,154,353 + +0.1 5,154,353 + +0.5 5,154,387 + +0.9 5,154,403 + -0.6 5,154,403 + -0.6 5,154,403 + +0.3 5,154,403 + +0.1 5,154,404 - -0.1 5,154,404 - +0.0 5,154,404 - -1.2 5,154,418 + -0.5 5,154,418 + -0.7 5,154,601 + PP_4538 0.22 +1.5 5,154,601 + PP_4538 0.22 -0.8 5,154,602 - PP_4538 0.22 +2.4 5,154,602 - PP_4538 0.22 +0.2 5,154,602 - PP_4538 0.22 -0.9 5,154,706 + PP_4538 0.40 +0.2 5,154,707 - PP_4538 0.40 -0.4 5,154,707 - PP_4538 0.40 -0.9 5,154,707 - PP_4538 0.40 +1.4 5,154,707 - PP_4538 0.40 -0.4 5,154,707 - PP_4538 0.40 +0.2 5,154,778 + PP_4538 0.52 -0.5 5,154,779 - PP_4538 0.52 -0.6 5,154,801 + PP_4538 0.56 -0.2 5,154,933 + PP_4538 0.78 +0.1 5,154,933 + PP_4538 0.78 -0.2 5,154,934 - PP_4538 0.78 -1.4 5,154,934 - PP_4538 0.78 +1.2 5,155,118 + +0.1 5,155,119 - +0.1 5,155,119 - +0.8 5,155,119 - -1.0 5,155,137 - +0.6
Or see this region's nucleotide sequence