Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4165 and PP_4166 are separated by 17 nucleotides PP_4166 and PP_4167 are separated by 162 nucleotides PP_4167 and PP_4168 overlap by 4 nucleotides PP_4168 and PP_4169 are separated by 36 nucleotides
PP_4165: PP_4165 - conserved protein of unknown function, at 4,708,259 to 4,709,122
_4165
PP_4166: PP_4166 - conserved protein of unknown function, at 4,709,140 to 4,709,514
_4166
PP_4167: PP_4167 - Phosphohistidine phosphatase SixA, at 4,709,677 to 4,710,135
_4167
PP_4168: PP_4168 - conserved protein of unknown function, at 4,710,132 to 4,710,479
_4168
PP_4169: PP_4169 - Glycerol-3-phosphate dehydrogenase [NAD(P)+], at 4,710,516 to 4,711,541
_4169
Position (kb) 4709
4710
4711 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4708.783 kb on - strand, within PP_4165 at 4708.962 kb on + strand, within PP_4165 at 4708.963 kb on - strand, within PP_4165 at 4708.995 kb on + strand, within PP_4165 at 4708.995 kb on + strand, within PP_4165 at 4708.996 kb on - strand, within PP_4165 at 4709.113 kb on + strand at 4709.113 kb on + strand at 4709.193 kb on - strand, within PP_4166 at 4709.240 kb on + strand, within PP_4166 at 4709.240 kb on + strand, within PP_4166 at 4709.320 kb on - strand, within PP_4166 at 4709.397 kb on - strand, within PP_4166 at 4709.433 kb on - strand, within PP_4166 at 4709.455 kb on - strand, within PP_4166 at 4709.561 kb on + strand at 4709.619 kb on + strand at 4709.811 kb on + strand, within PP_4167 at 4709.812 kb on - strand, within PP_4167 at 4709.812 kb on - strand, within PP_4167 at 4709.815 kb on + strand, within PP_4167 at 4709.815 kb on + strand, within PP_4167 at 4709.815 kb on + strand, within PP_4167 at 4709.815 kb on + strand, within PP_4167 at 4709.816 kb on - strand, within PP_4167 at 4709.816 kb on - strand, within PP_4167 at 4709.816 kb on - strand, within PP_4167 at 4709.816 kb on - strand, within PP_4167 at 4709.980 kb on + strand, within PP_4167 at 4709.980 kb on + strand, within PP_4167 at 4709.981 kb on - strand, within PP_4167 at 4709.984 kb on + strand, within PP_4167 at 4709.985 kb on - strand, within PP_4167 at 4709.985 kb on - strand, within PP_4167 at 4709.985 kb on - strand, within PP_4167 at 4710.337 kb on + strand, within PP_4168 at 4710.338 kb on - strand, within PP_4168 at 4710.338 kb on - strand, within PP_4168 at 4710.360 kb on + strand, within PP_4168 at 4710.360 kb on + strand, within PP_4168 at 4710.361 kb on - strand, within PP_4168 at 4710.471 kb on - strand at 4710.536 kb on - strand at 4710.646 kb on + strand, within PP_4169 at 4710.646 kb on + strand, within PP_4169 at 4710.646 kb on + strand at 4710.647 kb on - strand, within PP_4169 at 4710.647 kb on - strand, within PP_4169
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 4,708,783 - PP_4165 0.61 -1.9 4,708,962 + PP_4165 0.81 -0.3 4,708,963 - PP_4165 0.81 +0.8 4,708,995 + PP_4165 0.85 +0.6 4,708,995 + PP_4165 0.85 +1.4 4,708,996 - PP_4165 0.85 -0.2 4,709,113 + -0.1 4,709,113 + +0.7 4,709,193 - PP_4166 0.14 +0.2 4,709,240 + PP_4166 0.27 -0.2 4,709,240 + PP_4166 0.27 +1.2 4,709,320 - PP_4166 0.48 -0.8 4,709,397 - PP_4166 0.69 +1.7 4,709,433 - PP_4166 0.78 -0.4 4,709,455 - PP_4166 0.84 +0.2 4,709,561 + -0.5 4,709,619 + +0.5 4,709,811 + PP_4167 0.29 -0.9 4,709,812 - PP_4167 0.29 +0.6 4,709,812 - PP_4167 0.29 +0.7 4,709,815 + PP_4167 0.30 +0.1 4,709,815 + PP_4167 0.30 -0.9 4,709,815 + PP_4167 0.30 +2.4 4,709,815 + PP_4167 0.30 +1.2 4,709,816 - PP_4167 0.30 +0.1 4,709,816 - PP_4167 0.30 -0.3 4,709,816 - PP_4167 0.30 +0.9 4,709,816 - PP_4167 0.30 +0.7 4,709,980 + PP_4167 0.66 +0.5 4,709,980 + PP_4167 0.66 -0.2 4,709,981 - PP_4167 0.66 +0.4 4,709,984 + PP_4167 0.67 +1.4 4,709,985 - PP_4167 0.67 -0.5 4,709,985 - PP_4167 0.67 +0.7 4,709,985 - PP_4167 0.67 +1.4 4,710,337 + PP_4168 0.59 -0.5 4,710,338 - PP_4168 0.59 -0.8 4,710,338 - PP_4168 0.59 -0.4 4,710,360 + PP_4168 0.66 -0.2 4,710,360 + PP_4168 0.66 +0.1 4,710,361 - PP_4168 0.66 -0.5 4,710,471 - -0.6 4,710,536 - -0.1 4,710,646 + PP_4169 0.13 +3.7 4,710,646 + PP_4169 0.13 -2.2 4,710,646 + +3.7 4,710,647 - PP_4169 0.13 +1.7 4,710,647 - PP_4169 0.13 +2.1
Or see this region's nucleotide sequence