Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3927 and PP_5648 are separated by 169 nucleotides PP_5648 and PP_3928 are separated by 117 nucleotides PP_3928 and PP_3929 are separated by 162 nucleotides PP_3929 and PP_3930 are separated by 73 nucleotides
PP_3927: PP_3927 - Methyltransferase type 12, at 4,432,103 to 4,432,924
_3927
PP_5648: PP_5648 - conserved exported protein of unknown function, at 4,433,094 to 4,433,444
_5648
PP_3928: PP_3928 - conserved exported protein of unknown function, at 4,433,562 to 4,433,852
_3928
PP_3929: PP_3929 - conserved protein of unknown function, at 4,434,015 to 4,434,281
_3929
PP_3930: PP_3930 - conserved exported protein of unknown function, at 4,434,355 to 4,434,747
_3930
Position (kb)
4433
4434 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4432.571 kb on - strand, within PP_3927 at 4432.583 kb on + strand, within PP_3927 at 4432.584 kb on - strand, within PP_3927 at 4432.584 kb on - strand, within PP_3927 at 4432.584 kb on - strand, within PP_3927 at 4432.584 kb on - strand, within PP_3927 at 4432.617 kb on + strand, within PP_3927 at 4432.618 kb on - strand, within PP_3927 at 4432.851 kb on + strand at 4432.851 kb on + strand at 4432.852 kb on - strand at 4432.852 kb on - strand at 4432.852 kb on - strand at 4432.852 kb on - strand at 4432.895 kb on + strand at 4432.895 kb on + strand at 4432.895 kb on + strand at 4432.896 kb on - strand at 4432.896 kb on - strand at 4432.897 kb on + strand at 4432.897 kb on + strand at 4432.898 kb on - strand at 4432.898 kb on - strand at 4432.898 kb on - strand at 4432.898 kb on - strand at 4432.951 kb on + strand at 4432.951 kb on + strand at 4432.951 kb on + strand at 4432.951 kb on + strand at 4432.951 kb on + strand at 4432.951 kb on + strand at 4432.951 kb on + strand at 4432.951 kb on + strand at 4432.952 kb on - strand at 4432.952 kb on - strand at 4432.952 kb on - strand at 4432.952 kb on - strand at 4432.952 kb on - strand at 4432.952 kb on - strand at 4433.048 kb on - strand at 4433.155 kb on + strand, within PP_5648 at 4433.158 kb on + strand, within PP_5648 at 4433.159 kb on - strand, within PP_5648 at 4433.220 kb on - strand, within PP_5648 at 4433.258 kb on - strand, within PP_5648 at 4433.534 kb on + strand at 4433.574 kb on + strand at 4433.574 kb on + strand at 4433.575 kb on - strand at 4433.575 kb on - strand at 4433.575 kb on - strand at 4433.575 kb on - strand at 4433.703 kb on + strand, within PP_3928 at 4433.796 kb on + strand, within PP_3928 at 4433.796 kb on + strand, within PP_3928 at 4433.796 kb on + strand, within PP_3928 at 4433.796 kb on + strand, within PP_3928 at 4433.796 kb on + strand, within PP_3928 at 4433.797 kb on - strand, within PP_3928 at 4433.797 kb on - strand, within PP_3928 at 4433.797 kb on - strand, within PP_3928 at 4433.797 kb on - strand, within PP_3928 at 4433.797 kb on - strand, within PP_3928 at 4433.797 kb on - strand, within PP_3928 at 4433.797 kb on - strand, within PP_3928 at 4433.797 kb on - strand, within PP_3928 at 4433.928 kb on - strand at 4433.928 kb on - strand at 4433.928 kb on - strand at 4434.054 kb on - strand, within PP_3929 at 4434.073 kb on + strand, within PP_3929 at 4434.090 kb on + strand, within PP_3929 at 4434.091 kb on - strand, within PP_3929 at 4434.091 kb on - strand, within PP_3929 at 4434.092 kb on + strand, within PP_3929 at 4434.093 kb on - strand, within PP_3929 at 4434.169 kb on - strand, within PP_3929 at 4434.270 kb on + strand at 4434.270 kb on + strand at 4434.270 kb on + strand at 4434.271 kb on - strand at 4434.273 kb on + strand at 4434.273 kb on + strand at 4434.273 kb on + strand at 4434.273 kb on + strand at 4434.274 kb on - strand at 4434.274 kb on - strand at 4434.351 kb on + strand at 4434.395 kb on + strand, within PP_3930 at 4434.395 kb on + strand, within PP_3930 at 4434.396 kb on - strand, within PP_3930 at 4434.396 kb on - strand at 4434.626 kb on + strand, within PP_3930 at 4434.810 kb on + strand at 4434.832 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 4,432,571 - PP_3927 0.57 -0.0 4,432,583 + PP_3927 0.58 -0.3 4,432,584 - PP_3927 0.59 +0.4 4,432,584 - PP_3927 0.59 -0.2 4,432,584 - PP_3927 0.59 -1.1 4,432,584 - PP_3927 0.59 -0.3 4,432,617 + PP_3927 0.63 -0.2 4,432,618 - PP_3927 0.63 -0.1 4,432,851 + +0.2 4,432,851 + +1.5 4,432,852 - +0.3 4,432,852 - +0.5 4,432,852 - -0.7 4,432,852 - -1.1 4,432,895 + -0.0 4,432,895 + -0.2 4,432,895 + +0.2 4,432,896 - -0.3 4,432,896 - -0.2 4,432,897 + -0.2 4,432,897 + -1.8 4,432,898 - -1.5 4,432,898 - -0.2 4,432,898 - -0.4 4,432,898 - +1.1 4,432,951 + -0.1 4,432,951 + +0.4 4,432,951 + -0.3 4,432,951 + +1.0 4,432,951 + +1.7 4,432,951 + -1.4 4,432,951 + +0.1 4,432,951 + +1.4 4,432,952 - -0.2 4,432,952 - +0.8 4,432,952 - +0.1 4,432,952 - +0.2 4,432,952 - +0.3 4,432,952 - -0.1 4,433,048 - -0.3 4,433,155 + PP_5648 0.17 -1.1 4,433,158 + PP_5648 0.18 -1.9 4,433,159 - PP_5648 0.19 +1.3 4,433,220 - PP_5648 0.36 -0.2 4,433,258 - PP_5648 0.47 -1.2 4,433,534 + -0.4 4,433,574 + -0.1 4,433,574 + -0.5 4,433,575 - -0.1 4,433,575 - -0.2 4,433,575 - +0.4 4,433,575 - +3.7 4,433,703 + PP_3928 0.48 -0.3 4,433,796 + PP_3928 0.80 +1.2 4,433,796 + PP_3928 0.80 +0.6 4,433,796 + PP_3928 0.80 +0.9 4,433,796 + PP_3928 0.80 +1.1 4,433,796 + PP_3928 0.80 -1.4 4,433,797 - PP_3928 0.81 +0.0 4,433,797 - PP_3928 0.81 +1.6 4,433,797 - PP_3928 0.81 +0.1 4,433,797 - PP_3928 0.81 -0.9 4,433,797 - PP_3928 0.81 +0.2 4,433,797 - PP_3928 0.81 +0.2 4,433,797 - PP_3928 0.81 +1.1 4,433,797 - PP_3928 0.81 +0.9 4,433,928 - +2.7 4,433,928 - -0.4 4,433,928 - -1.6 4,434,054 - PP_3929 0.15 +2.1 4,434,073 + PP_3929 0.22 -0.5 4,434,090 + PP_3929 0.28 -0.2 4,434,091 - PP_3929 0.28 -1.5 4,434,091 - PP_3929 0.28 +0.7 4,434,092 + PP_3929 0.29 -0.9 4,434,093 - PP_3929 0.29 -1.1 4,434,169 - PP_3929 0.58 -0.4 4,434,270 + +0.5 4,434,270 + -1.7 4,434,270 + +0.5 4,434,271 - +0.4 4,434,273 + +2.1 4,434,273 + -0.6 4,434,273 + -1.5 4,434,273 + -0.2 4,434,274 - -0.7 4,434,274 - -0.6 4,434,351 + +1.7 4,434,395 + PP_3930 0.10 +2.4 4,434,395 + PP_3930 0.10 -1.1 4,434,396 - PP_3930 0.10 +1.6 4,434,396 - +0.4 4,434,626 + PP_3930 0.69 +1.0 4,434,810 + +0.9 4,434,832 + +0.6
Or see this region's nucleotide sequence