Strain Fitness in Pseudomonas putida KT2440 around PP_3881

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
4,400,575	-		PP_3879	0.54	+0.3
4,400,575	-		PP_3879	0.54	-0.2
4,400,641	-		PP_3879	0.59	+1.0
4,400,641	-		PP_3879	0.59	-0.4
4,400,641	-		PP_3879	0.59	-0.4
4,400,641	-		PP_3879	0.59	+0.4
4,400,815	-		PP_3879	0.73	-0.3
4,400,865	+		PP_3879	0.77	-1.6
4,400,866	-				-0.1
4,400,907	+		PP_3879	0.80	-0.3
4,400,907	+		PP_3879	0.80	-0.6
4,400,907	+		PP_3879	0.80	-0.1
4,400,972	+		PP_3879	0.85	-0.3
4,400,973	-		PP_3879	0.85	-0.4
4,400,981	+		PP_3879	0.86	+0.7
4,401,023	-		PP_3879	0.89	+0.1
4,401,023	-		PP_3879	0.89	+0.3
4,401,157	+				-1.0
4,401,157	+				+0.1
4,401,157	+				-0.6
4,401,158	-				+1.0
4,401,158	-				-0.7
4,401,158	-				-0.2
4,401,158	-				-0.2
4,401,190	+		PP_3880	0.16	-1.2
4,401,355	-				-0.4
4,401,445	-				+1.3
4,401,532	-		PP_3881	0.10	-1.2
4,401,532	-		PP_3881	0.10	+0.7
4,401,663	+		PP_3881	0.18	-1.3
4,401,677	+		PP_3881	0.18	+1.1
4,401,727	+		PP_3881	0.21	+0.9
4,401,806	+		PP_3881	0.25	-0.0
4,401,806	+		PP_3881	0.25	-1.2
4,401,949	+		PP_3881	0.33	-0.4
4,401,979	+		PP_3881	0.35	+0.6
4,401,980	-		PP_3881	0.35	-0.5
4,401,980	-		PP_3881	0.35	-1.0
4,401,980	-		PP_3881	0.35	+0.1
4,401,980	-		PP_3881	0.35	+1.0
4,402,022	+		PP_3881	0.37	-0.4
4,402,022	+		PP_3881	0.37	+0.1
4,402,022	+		PP_3881	0.37	-0.3
4,402,023	-		PP_3881	0.37	+0.6
4,402,216	+		PP_3881	0.48	+0.7
4,402,216	+		PP_3881	0.48	+1.6
4,402,217	-		PP_3881	0.48	-1.1
4,402,314	+		PP_3881	0.54	-1.7
4,402,316	+		PP_3881	0.54	-2.0
4,402,316	+		PP_3881	0.54	-0.6
4,402,316	+		PP_3881	0.54	-1.6
4,402,316	+		PP_3881	0.54	+0.1
4,402,317	-		PP_3881	0.54	-0.2
4,402,317	-		PP_3881	0.54	-0.6
4,402,317	-		PP_3881	0.54	-0.2
4,402,318	+		PP_3881	0.54	-0.8
4,402,318	+		PP_3881	0.54	+0.3
4,402,318	+		PP_3881	0.54	-1.3
4,402,318	+		PP_3881	0.54	-1.7
4,402,318	+		PP_3881	0.54	+0.1
4,402,318	+		PP_3881	0.54	+0.2
4,402,319	-		PP_3881	0.54	-0.5
4,402,319	-		PP_3881	0.54	+0.6
4,402,369	+		PP_3881	0.57	-0.1
4,402,588	+		PP_3881	0.69	+0.5
4,402,588	+		PP_3881	0.69	-0.9
4,402,720	+		PP_3881	0.76	-0.2
4,402,720	+		PP_3881	0.76	-0.1
4,402,720	+		PP_3881	0.76	-1.5
4,402,721	-		PP_3881	0.76	-0.4
4,402,721	-		PP_3881	0.76	+0.7
4,402,817	-		PP_3881	0.81	-0.7
4,402,961	+		PP_3881	0.89	-0.5
4,402,961	+		PP_3881	0.89	-1.2
4,402,961	+		PP_3881	0.89	-1.0
4,402,961	+		PP_3881	0.89	-1.4
4,402,962	-		PP_3881	0.89	+0.1
4,403,161	+		PP_3882	0.10	+0.1
4,403,231	-		PP_3882	0.25	-0.0
4,403,231	-		PP_3882	0.25	+0.2
4,403,300	+		PP_3882	0.40	-1.5
4,403,301	-		PP_3882	0.40	-0.6
4,403,352	-		PP_3882	0.51	-0.8
4,403,487	+		PP_3882	0.80	-0.4
4,403,488	-		PP_3882	0.80	+0.2
4,403,727	+				-0.3
4,403,763	+		PP_3883	0.15	-0.6
4,404,000	-		PP_3883	0.88	+0.6
4,404,000	-		PP_3883	0.88	+0.2
4,404,094	+		PP_3884	0.17	+1.0
4,404,095	-		PP_3884	0.17	+0.8