Strain Fitness in Pseudomonas putida KT2440 around PP_3441

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,897,022	+		PP_3440	0.21	-0.7
3,897,333	+		PP_3440	0.48	+1.8
3,897,334	-		PP_3440	0.48	+1.3
3,897,414	+		PP_3440	0.56	-1.5
3,897,414	+		PP_3440	0.56	-0.7
3,897,415	-		PP_3440	0.56	-0.3
3,897,415	-		PP_3440	0.56	-0.2
3,897,415	-		PP_3440	0.56	+0.7
3,897,465	-		PP_3440	0.60	-0.2
3,897,474	+		PP_3440	0.61	+0.4
3,897,524	-		PP_3440	0.65	-0.3
3,897,524	-		PP_3440	0.65	+0.0
3,897,564	+		PP_3440	0.69	-0.1
3,897,564	+		PP_3440	0.69	+1.4
3,897,564	+		PP_3440	0.69	+0.2
3,897,564	+		PP_3440	0.69	-0.5
3,897,587	+		PP_3440	0.71	-1.6
3,897,587	+		PP_3440	0.71	+1.6
3,897,587	+		PP_3440	0.71	+0.9
3,897,587	+		PP_3440	0.71	+0.7
3,897,587	+		PP_3440	0.71	+0.7
3,897,587	+		PP_3440	0.71	-0.5
3,897,588	-		PP_3440	0.71	-0.1
3,897,588	-		PP_3440	0.71	-2.5
3,897,588	-		PP_3440	0.71	-1.5
3,897,588	-		PP_3440	0.71	-0.3
3,897,604	-		PP_3440	0.73	-0.9
3,897,635	-		PP_3440	0.75	+3.7
3,897,834	+				-1.2
3,897,834	+				+0.1
3,897,834	+				-1.2
3,897,834	+				-0.2
3,897,834	+				+1.7
3,897,835	-				-1.1
3,897,835	-				+0.6
3,897,840	+				+3.7
3,897,981	+				-0.2
3,897,990	-				-0.9
3,898,033	+				-0.7
3,898,085	-				-0.8
3,898,085	-				-1.9
3,898,090	+				-0.6
3,898,090	+				+1.4
3,898,091	-				+0.4
3,898,091	-				-0.6
3,898,264	+		PP_3441	0.24	+1.4
3,898,265	-		PP_3441	0.24	+0.0
3,898,360	+		PP_3441	0.33	+1.1
3,898,360	+		PP_3441	0.33	+0.9
3,898,360	+		PP_3441	0.33	+0.1
3,898,360	+		PP_3441	0.33	-0.5
3,898,361	-		PP_3441	0.33	+0.5
3,898,361	-		PP_3441	0.33	+0.6
3,898,364	-		PP_3441	0.33	-1.1
3,898,364	-		PP_3441	0.33	+0.3
3,898,364	-		PP_3441	0.33	+1.2
3,898,462	+		PP_3441	0.41	+1.6
3,898,462	+		PP_3441	0.41	+0.6
3,898,503	+		PP_3441	0.45	+0.7
3,898,642	+		PP_3441	0.57	+3.7
3,898,643	-		PP_3441	0.57	+2.1
3,898,709	-		PP_3441	0.63	-1.7
3,898,807	+		PP_3441	0.71	+1.7
3,898,808	-		PP_3441	0.71	+0.4
3,898,808	-		PP_3441	0.71	+0.9
3,898,969	+		PP_3441	0.85	+1.5
3,898,969	+		PP_3441	0.85	+1.3
3,898,970	-		PP_3441	0.85	-0.3
3,898,970	-		PP_3441	0.85	-0.0
3,899,374	-				+0.5
3,899,375	+				+0.2
3,899,436	-				-0.2
3,899,588	+				-0.1
3,899,680	+				+0.1
3,899,680	+				+0.2
3,899,681	-				+0.0
3,899,777	+		PP_3443	0.11	-0.6
3,899,778	-		PP_3443	0.11	+0.2
3,899,837	+		PP_3443	0.15	-1.4
3,900,019	+		PP_3443	0.26	+0.8
3,900,020	-		PP_3443	0.26	+0.4