Strain Fitness in Pseudomonas putida KT2440 around PP_3309

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPP_3307 and PP_3308 are separated by 111 nucleotidesPP_3308 and PP_3309 overlap by 4 nucleotidesPP_3309 and PP_3310 overlap by 4 nucleotides PP_3307: PP_3307 - conserved protein of unknown function, at 3,742,701 to 3,743,081 _3307 PP_3308: PP_3308 - promiscuous aromatic aldehyde dehydrogenase, 2Fe-2S subunit, at 3,743,193 to 3,743,720 _3308 PP_3309: PP_3309 - promiscuous aromatic aldehyde dehydrogenase, FAD-binding subunit, at 3,743,717 to 3,744,718 _3309 PP_3310: PP_3310 - promiscuous aromatic aldehyde dehydrogenase, molybdopterin-binding subunit, at 3,744,715 to 3,746,931 _3310 Position (kb) 3743 3744 3745Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3742.725 kb on + strandat 3742.725 kb on + strandat 3742.725 kb on + strandat 3742.726 kb on - strandat 3742.726 kb on - strandat 3742.771 kb on - strandat 3742.995 kb on - strand, within PP_3307at 3742.995 kb on - strand, within PP_3307at 3743.189 kb on + strandat 3743.189 kb on + strandat 3743.580 kb on + strand, within PP_3308at 3743.637 kb on + strand, within PP_3308at 3743.637 kb on + strand, within PP_3308at 3743.638 kb on - strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.639 kb on + strand, within PP_3308at 3743.640 kb on - strand, within PP_3308at 3743.640 kb on - strand, within PP_3308at 3743.640 kb on - strand, within PP_3308at 3743.640 kb on - strand, within PP_3308at 3743.640 kb on - strand, within PP_3308at 3743.972 kb on + strand, within PP_3309at 3744.058 kb on + strand, within PP_3309at 3744.059 kb on - strand, within PP_3309at 3744.068 kb on + strand, within PP_3309at 3744.069 kb on - strand, within PP_3309at 3744.069 kb on - strand, within PP_3309at 3744.071 kb on + strand, within PP_3309at 3744.072 kb on - strand, within PP_3309at 3744.072 kb on - strand, within PP_3309at 3744.077 kb on + strand, within PP_3309at 3744.078 kb on - strand, within PP_3309at 3744.202 kb on + strand, within PP_3309at 3744.202 kb on + strand, within PP_3309at 3744.202 kb on + strand, within PP_3309at 3744.270 kb on + strand, within PP_3309at 3744.270 kb on + strand, within PP_3309at 3744.741 kb on + strandat 3744.758 kb on + strandat 3744.758 kb on + strandat 3744.846 kb on + strandat 3744.846 kb on + strandat 3744.847 kb on - strandat 3744.847 kb on - strandat 3744.847 kb on - strandat 3744.882 kb on + strandat 3744.942 kb on + strand, within PP_3310at 3744.942 kb on + strand, within PP_3310at 3744.942 kb on + strand, within PP_3310at 3744.943 kb on - strand, within PP_3310at 3744.943 kb on - strand, within PP_3310at 3744.943 kb on - strand, within PP_3310at 3744.943 kb on - strand, within PP_3310at 3744.964 kb on + strand, within PP_3310at 3744.965 kb on - strand, within PP_3310at 3745.108 kb on + strand, within PP_3310at 3745.240 kb on + strand, within PP_3310at 3745.241 kb on - strand, within PP_3310at 3745.258 kb on + strandat 3745.258 kb on + strand, within PP_3310at 3745.293 kb on - strand, within PP_3310at 3745.297 kb on + strand, within PP_3310at 3745.360 kb on + strand, within PP_3310at 3745.361 kb on - strand, within PP_3310at 3745.395 kb on + strand, within PP_3310at 3745.396 kb on - strand, within PP_3310at 3745.396 kb on - strand, within PP_3310at 3745.453 kb on + strand, within PP_3310at 3745.684 kb on + strand, within PP_3310at 3745.685 kb on - strand, within PP_3310at 3745.711 kb on + strand, within PP_3310

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
remove
3,742,725 + -0.4
3,742,725 + +2.1
3,742,725 + -0.9
3,742,726 - -0.2
3,742,726 - +0.3
3,742,771 - +0.6
3,742,995 - PP_3307 0.77 -0.6
3,742,995 - PP_3307 0.77 -0.2
3,743,189 + -1.2
3,743,189 + -1.8
3,743,580 + PP_3308 0.73 +0.2
3,743,637 + PP_3308 0.84 -1.9
3,743,637 + PP_3308 0.84 +0.3
3,743,638 - PP_3308 0.84 -0.9
3,743,639 + PP_3308 0.84 -0.1
3,743,639 + PP_3308 0.84 -0.5
3,743,639 + PP_3308 0.84 -0.4
3,743,639 + PP_3308 0.84 +0.8
3,743,639 + PP_3308 0.84 -0.3
3,743,639 + PP_3308 0.84 -0.5
3,743,639 + PP_3308 0.84 +1.3
3,743,639 + PP_3308 0.84 +0.3
3,743,639 + PP_3308 0.84 -0.3
3,743,639 + PP_3308 0.84 -0.5
3,743,639 + PP_3308 0.84 -0.0
3,743,639 + PP_3308 0.84 +0.4
3,743,640 - PP_3308 0.85 -1.0
3,743,640 - PP_3308 0.85 +0.0
3,743,640 - PP_3308 0.85 -0.9
3,743,640 - PP_3308 0.85 +0.7
3,743,640 - PP_3308 0.85 -0.3
3,743,972 + PP_3309 0.25 -0.1
3,744,058 + PP_3309 0.34 +0.1
3,744,059 - PP_3309 0.34 -0.2
3,744,068 + PP_3309 0.35 -0.1
3,744,069 - PP_3309 0.35 -0.1
3,744,069 - PP_3309 0.35 +1.2
3,744,071 + PP_3309 0.35 +1.6
3,744,072 - PP_3309 0.35 +0.6
3,744,072 - PP_3309 0.35 +0.4
3,744,077 + PP_3309 0.36 +0.6
3,744,078 - PP_3309 0.36 +0.0
3,744,202 + PP_3309 0.48 +0.6
3,744,202 + PP_3309 0.48 +0.2
3,744,202 + PP_3309 0.48 +0.3
3,744,270 + PP_3309 0.55 +0.1
3,744,270 + PP_3309 0.55 +0.1
3,744,741 + -0.9
3,744,758 + -0.4
3,744,758 + +0.3
3,744,846 + +0.1
3,744,846 + -0.7
3,744,847 - -0.4
3,744,847 - +0.0
3,744,847 - -0.3
3,744,882 + +2.7
3,744,942 + PP_3310 0.10 +0.1
3,744,942 + PP_3310 0.10 -0.8
3,744,942 + PP_3310 0.10 -0.1
3,744,943 - PP_3310 0.10 +0.1
3,744,943 - PP_3310 0.10 -1.7
3,744,943 - PP_3310 0.10 -1.0
3,744,943 - PP_3310 0.10 +1.2
3,744,964 + PP_3310 0.11 -0.0
3,744,965 - PP_3310 0.11 -1.1
3,745,108 + PP_3310 0.18 +0.3
3,745,240 + PP_3310 0.24 +0.3
3,745,241 - PP_3310 0.24 +3.7
3,745,258 + +2.7
3,745,258 + PP_3310 0.24 -0.0
3,745,293 - PP_3310 0.26 +0.7
3,745,297 + PP_3310 0.26 -0.6
3,745,360 + PP_3310 0.29 -0.4
3,745,361 - PP_3310 0.29 -0.0
3,745,395 + PP_3310 0.31 -0.4
3,745,396 - PP_3310 0.31 +0.3
3,745,396 - PP_3310 0.31 +2.7
3,745,453 + PP_3310 0.33 -0.3
3,745,684 + PP_3310 0.44 +1.0
3,745,685 - PP_3310 0.44 +1.2
3,745,711 + PP_3310 0.45 +1.5

Or see this region's nucleotide sequence