Strain Fitness in Pseudomonas putida KT2440 around PP_3069

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,450,016	-				+0.1
3,450,130	+				+0.4
3,450,131	-				+0.1
3,450,137	+				+0.5
3,450,137	+				-0.5
3,450,138	-				+1.7
3,450,265	+		PP_5558	0.12	-1.2
3,450,265	+		PP_5558	0.12	-0.5
3,450,438	-		PP_5558	0.59	-0.5
3,450,438	-		PP_5558	0.59	-0.8
3,450,438	-				+1.1
3,450,562	+				+1.1
3,450,562	+				-0.7
3,450,562	+				+0.2
3,450,563	-				-0.8
3,450,563	-				-0.5
3,450,597	-				+1.0
3,450,602	-				-0.1
3,450,615	-				+0.0
3,450,633	+				+1.4
3,450,642	+				+0.7
3,450,642	+				+0.2
3,450,642	+				+0.4
3,450,642	+				-1.9
3,450,642	+				+0.3
3,450,643	-				-0.2
3,450,663	-				-0.3
3,450,681	-				-0.5
3,450,847	+				-0.1
3,450,847	+				-0.3
3,450,848	-				+2.7
3,450,865	+				-0.5
3,450,865	+				-0.9
3,450,866	-				+0.3
3,450,866	-				-1.8
3,451,040	-				+0.2
3,451,040	-				-0.1
3,451,040	-				+2.0
3,451,084	-				+0.9
3,451,084	-				-0.9
3,451,112	+				-0.3
3,451,112	+				+0.3
3,451,112	+				+0.2
3,451,113	-				-0.2
3,451,483	+		PP_3069	0.21	-1.0
3,451,484	-		PP_3069	0.21	-0.3
3,451,504	+		PP_3069	0.22	+0.5
3,451,535	-		PP_3069	0.23	+0.1
3,451,547	+		PP_3069	0.23	-0.7
3,451,629	-		PP_3069	0.27	-1.6
3,451,635	+		PP_3069	0.27	-1.3
3,451,635	+		PP_3069	0.27	-1.3
3,451,636	-		PP_3069	0.27	-0.5
3,451,636	-		PP_3069	0.27	-1.0
3,451,639	+		PP_3069	0.27	-1.2
3,451,639	+		PP_3069	0.27	-1.0
3,451,639	+		PP_3069	0.27	+1.8
3,451,639	+		PP_3069	0.27	+0.5
3,451,640	-		PP_3069	0.27	-0.1
3,451,640	-		PP_3069	0.27	-0.4
3,451,640	-		PP_3069	0.27	+0.9
3,451,640	-		PP_3069	0.27	-0.6
3,451,640	-		PP_3069	0.27	-0.6
3,451,640	-		PP_3069	0.27	+0.8
3,451,643	+		PP_3069	0.27	-1.7
3,451,684	+		PP_3069	0.29	-0.6
3,451,685	-		PP_3069	0.29	+0.8
3,451,685	-		PP_3069	0.29	-2.1
3,451,774	+		PP_3069	0.32	-1.2
3,451,775	-		PP_3069	0.33	+0.7
3,451,819	-		PP_3069	0.34	-1.0
3,451,900	+		PP_3069	0.38	-1.2
3,451,901	-		PP_3069	0.38	+0.1
3,452,080	+		PP_3069	0.45	+0.1
3,452,080	+		PP_3069	0.45	+0.1
3,452,081	-		PP_3069	0.45	+0.3
3,452,081	-		PP_3069	0.45	+0.3
3,452,081	-		PP_3069	0.45	-0.5
3,452,134	+				+1.7
3,452,134	+		PP_3069	0.47	+0.1
3,452,135	-		PP_3069	0.47	+0.1
3,452,163	+		PP_3069	0.48	+0.0
3,452,164	-		PP_3069	0.48	+0.2
3,452,273	+		PP_3069	0.53	+0.5
3,452,273	+		PP_3069	0.53	-0.4
3,452,620	+		PP_3069	0.67	-0.6
3,452,620	+		PP_3069	0.67	-0.7
3,452,621	-		PP_3069	0.67	-1.3
3,452,621	-		PP_3069	0.67	-0.7
3,452,621	-		PP_3069	0.67	+0.2
3,452,621	-		PP_3069	0.67	-1.0
3,452,670	-		PP_3069	0.69	-0.9
3,452,670	-		PP_3069	0.69	-0.9
3,452,694	-		PP_3069	0.70	-0.0
3,452,694	-		PP_3069	0.70	-1.3
3,452,711	+		PP_3069	0.70	-0.6
3,452,768	+		PP_3069	0.73	-0.7
3,452,911	+		PP_3069	0.78	-1.6
3,452,911	+		PP_3069	0.78	+0.2
3,452,912	-		PP_3069	0.78	-0.4
3,453,322	+				-0.3
3,453,322	+				+0.1
3,453,322	+				-0.2
3,453,322	+				+1.2
3,453,322	+				-0.1
3,453,323	-				-0.0
3,453,323	-				+2.6
3,453,323	-				-0.0
3,453,323	-				-1.0
3,453,323	-				+0.2
3,453,323	-				-0.7
3,453,323	-				-1.8
3,453,353	+				+1.5
3,453,498	+				-0.7
3,453,498	+				-0.3
3,453,498	+				-0.5
3,453,498	+				+2.7
3,453,498	+				-0.2
3,453,499	-				+0.5
3,453,499	-				+0.1
3,453,499	-				+0.8
3,453,499	-				+0.0
3,453,499	-				+1.4
3,453,500	+				+0.2
3,453,500	+				-1.1
3,453,570	-				-0.2
3,453,840	-				+0.6
3,453,840	-				-0.6
3,453,868	+				+0.4
3,453,868	+				+0.2
3,453,869	-				+0.5
3,453,869	-				-0.7
3,454,076	+				+0.4
3,454,125	+				+0.5
3,454,133	+				-1.1
3,454,133	+				+1.7
3,454,282	-		PP_3071	0.18	-1.9
3,454,416	+		PP_3071	0.24	-0.8
3,454,416	+		PP_3071	0.24	+0.2
3,454,417	-		PP_3071	0.25	+0.3
3,454,417	-				+2.5
3,454,417	-		PP_3071	0.25	-0.1